TMPyP4-regulated cell proliferation and apoptosis through the Wnt/β-catenin signaling pathway in SW480 cells.

Background: The aim of this study was to investigate the potential effects of the 5, 10, 15, 20-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP4) on the proliferation and apoptosis of SW480 cells and the underlying mechanisms by which TMPyP4 exerted its actions.

Methods: After treated with different doses of TMPyP4, cell viability was determined by MTT method, the apoptosis was observed by flow cytometry (FCM) and the expression of Wnt, GSK-3β, β-catenin and cyclinD1 was measured by RT-PCR and Western blot analysis.

Results: The analysis revealed that TMPyP4 potently suppressed cell viability and induced the apoptosis of SW480 cells in a dose-dependent manner.

[Cloning and expression of actin gene of Toxoplasma gondii].

Objective: To clone and express the actin gene of Toxoplasma gondii, and analyze the immunoreactivity of the recombinant protein.

Methods: Total RNA was extracted from tachyzoites of RH strain of T. gondii.

Gene-cloning, expression and antigenicity analysis of rhoptry protein 17 of Toxoplasma gondii.

Objective: To clone and express the rhoptry protein 17 (ROP17) gene of RH strain of Toxoplasma gondii, analyze the antigenicity of recombinant protein.

Methods: Total RNA was extracted from tachyzoites of RH strain of T. gondii.

[Cloning, expression and antigenicity analysis of phosphoglycerate mutase 2 gene of Toxoplasma gondii].

Objective: To clone and express the phosphoglycerate mutase 2 (PGAM2) gene of Toxoplasma gondii, and analyze the antigenicity of the recombinant protein.

Methods: Total RNA was extracted from T. gondii tachyzoites of RH strain and reversely transcribed into cDNA.

[Effect of oral administration of tribendimidine at different dosages against Trichinella spiralis encapsulated larvae in mice].

Objective: To observe the efficacy of oral administration of tribendimidine (TBD) at different dosages against Trichinella spiralis encapsulated larvae in murine striated muscle.

Methods: A total of 88 BALB/c mice were divided equally into 11 groups. Each mouse was infected orally with 50 T spiralis encapsulated larvae.

[Kinetics of IgA secreting cells and sIgA in small intestine of mice induced by intranasal immunization with Toxoplasma gondii STAg].

Objective: To investigate the kinetics of IgA secreting cells (IgASCs) and secretory IgA (sIgA) level in small intestine induced by intranasal immunization with Toxoplasma gondii soluble tachyzoite antigen (STAg) in mice.

Methods: Ninety-six 5 to 6-week old BALB/c mice were randomly divided into immunity and control groups. Mice of the immunity group were each intranasally immunized with STAg 20 microg in 20 microl PBS, twice at an interval of 2 weeks, while the control mice were each given 20 microl PBS.

[Early kinetics of Toxoplasma gondii infection in mice infected intragastrically with tachyzoites by chromogenic in situ hybridization targeting SAG2 mRNA].

Objective: To observe the early kinetics of Toxoplasma gondii infection in mice inoculated with tachyzoites of RH strain.

Methods: Twenty BALB/c mice were administered intragastrically with tachyzoites of RH strain (2 x 10(4)/mice). Parasite burdens in mesenteric lymph node (MLN), liver, spleen, lung and brain were determined by chromogenic in situ hybridization targeting SAG2 mRNA at 1, 2, 4, 6 and 8 days postinfection.

[Kinetics of IgA secreting cells and IgA in small intestine of mice induced by Toxoplasma gondii tachyzoite infection].

Objective: To investigate the kinetics of IgA secreting cells (IgASCs) in small intestine and the specific antibody level induced by Toxoplasma gondii tachyzoite infection in mice.

Methods: Ninety-six BALB/c mice were randomly divided into 2 groups, 12 were intragastrically given 0.5 ml PBS as control, the rest were each intragastrically infected with 1 x 10(4) tachyzoites of the virulent RH strain Toxoplasma gondii.

[Dynamic observation of attachment and invasion of Toxoplasma gondii tachyzoites to intestinal mucosa in BALB/c mice by chromogenic in situ hybridization targeting SAG2 mRNA].

Objective: To observe dynamically the location and time of attachment and invasion of Toxoplasma gondii tachyzoites to murine intestinal mucosa by chromogenic in situ hybridization targeting SAG2 mRNA.

Methods: Thirty 7- to 8-week-old BALB/c mice were randomly divided into experiment group (24 mice) and control group (6 mice). Each animal in the experiment group was given 2 x 10(4) tachyzoites of RH stain in 0.