Chloroplast transformation of rapeseed (Brassica napus) by particle bombardment of cotyledons.

A protocol for chloroplast transformation of an elite rapeseed cultivar (Brassica napus L.) was developed based on optimized conditions for callus induction and regeneration from cotyledonary tissues. Comparison of six different media with three elite cultivars showed that B5 medium plus 3 mg/l AgNO(3) supplemented with 0.

Cloning and characterization of microRNAs from Brassica napus.

A library containing approximately 40,000 small RNA sequences was constructed for Brassica napus. Analysis of 3025 sequences obtained from this library resulted in the identification of 11 conserved miRNA families, which were validated by secondary structure prediction using surrounding sequences in the Brassica genome. Two 21 nt small RNA sequences reside within the arm of a pre-miRNA like stem-loop structure, making them likely candidates for novel non-conserved miRNAs in B.

Molecular validation of multiple allele inheritance for dominant genic male sterility gene in Brassica napus L.

Dominant genic male sterility (DGMS) has been playing an increasingly important role, not only as a tool for assisting in recurrent selection but also as an alternative approach for efficient production of hybrids. Previous studies indicate that fertility restoration of DGMS is the action of another unlinked dominant gene. Recently, through classical genetic analysis with various test populations we have verified that in a DGMS line 609AB the trait is inherited in a multiple allelic pattern.

[Improvement of microspores culture techniques in Brassica napus.L].

The application of microspore culture technique was restricted because of its low frequency of embryogenesis and chromosome doubling. Two methods of enhancing the frequency of embryogenesis were employed in the study,namely,activated charcoal treatment in NLN-13 media and 6-BA treatment in NLN-16 media. The treatment with 0.

Inheritance of seed colour and identification of RAPD and AFLP markers linked to the seed colour gene in rapeseed (Brassica napus L.).

In China Polima cytoplasmic male sterility (cms) is currently the most important hybrid system used for the breeding of hybrids. In an effort to develop yellow-seeded Polima cms restorer lines, we used yellow-seeded, doubled haploid (DH) line No.2127-17 as the gene source in crosses with two elite black-seeded Polima cms R lines, Hui5148-2 and 99Yu42, which originated from our breeding programme.

Identification of the linkage relationship between the flower colour and the content of erucic acid in the resynthesized Brassica napus L.

Brassica napus variety Quantum (yellow flower and low erucic content) as the female parent was crossed with a resynthesized Brassica napus line No. 2127-17 (white flower and high erucic content). The segregation ratios of the flower colour and the erucic acid content were analyzed in the F1, BC1, F2, and DH (doubled haploid) populations.

[RAPD analysis for the genetic diversity of Brassica rapa in Tibet].

Tibet, a most beautiful place, locating in southwestern China. She has been called as the third pole of the earth. Unique geological history, complex land surface and climatic zones, various soil types, all different wild vegetations etc.