Differentiation of human amniotic epithelial cells into corneal epithelial-like cells in vitro.

Aim: To explore the feasibility that human amniotic epithelial cells (hAECs) have the potential to differentiate into corneal epithelial-like cells under the microenvironment replicated by spontaneously immortalized human corneal epithelial cells (S-ihCECs).

Methods: hAECs were isolated by enzyme digestion, and flow cytometry was used to analysis the expression of CD29/90/166/73/34 and HLA-DR. Recovered and cultured S-ihCECs, immunocytochemistry was used to detect the expression of CK3/12.

Implantation of Iakymenko keratoprosthesis in patients with severe ocular injury.

Aim: To present the results of implantation of Iakymenko keratoprosthesis in five patients with vascularized corneal leukoma caused by severe ocular injury.

Methods: Iakymenko keratoprosthesis was implanted into 5 eyes of 5 patients: 4 patients were suffered from chemical burns and 1 patient from explosive injury. The preoperative visual acuity ranged from light perception to hand motion.

Construction of eukaryotic plasmid expressing human TGFBI and its influence on human corneal epithelial cells.

Aim: To detect the expression of transforming growth factor beta-induced gene (TGFBI) protein in human corneal tissue and overexpress it in the human corneal epithelial cells in order to discuss the function of TGFBI in the pathogenesis of corneal dystrophy.

Methods: Immunohistochemistry (IHC) was used to detect the expression of TGFBI in the human cornea tissue. TGFBI cDNA was obtained by reverse transcription-PCR from human corneal total RNA extracted from cornea transplant donor and cloned into pCMV-N-HA vector.

Endogenous aspergillus endophthalmitis after kidney transplantation.

Endogenous aspergillus endophthalmitis(EAE) after kidney transplant is a rare but important clinical problem due to potentially devastating consequences. Early diagnosis of EAE, timely removal of affected vitreous by vitrectomy, proper anti-fungal treatment, all contributed to the successful control of the disease. Therapeutic success of EAE in post-transplant patients depends largely on prompt diagnosis.

Troglitazone induced apoptosis of human pterygium fibroblasts through a mitochondrial-dependent pathway.

Aim: To study the effect of troglitazone on primary culture human pterygium fibroblasts (HPF).

Methods: Cell viability loss and apoptosis were quantified by cell counting kit-8, AnnexinV-FITC/PI double staining, caspases activity test and western blotting. Flow cytometry was used to detect mitochondrial membrane potential.

Pioglitazone inhibits TGFβ induced keratocyte transformation to myofibroblast and extracellular matrix production.

Phenotype transformation of corneal keratocyte to myofibroblast plays an important role in the wound healing process of cornea and TGFβ is considered to be the most important mediator to induce myofibroblast trans-differentiation. Peroxisome proliferator-activated receptors-γ (PPAR-γ) activation has been proved to exert anti-fibrotic effect in many tissues. In this study, we investigated the effect of PPAR-γ agonist, pioglitazone, on myofibroblast transformation, extracellular matrix production and cell proliferation.

[Preliminary study on human bone marrow mesenchymal stem cells differentiation into epithelial-like cells].

Objective: To investigate the differentiation potential of human bone marrow mesenchymal stem cells (hMSC) inducing into epithelial-like cells, even corneal epithelial-like cells, and to discuss the plasticity that make hMSC the seed cells used in corneal tissue engineering.

Methods: hMSC were isolated and purified by density gradient centrifugation combined with an attachment culture method and passaged in vitro. hMSC were identified by flow cytometry.

[Histopathology and ultrastructure changes of retina in rats exposed to n-hexane].

Objective: To investigate the injury in the retina of rats exposed to n-hexane.

Methods: Thirty-two SD male rats were randomly divided into control group and four n-hexane groups. The rats in the four n-hexane groups inhaled 35.

[Morphological changes of corneal nerve in rats exposed to n-hexane.].

Objective: To investigate the injury in the corneal nerve and cornea of rats exposed to n-hexane.

Methods: Thirty-two SD male rats were randomly divided into one control group and four n-hexane groups. The four n-hexane groups inhaled 35.

[Evaluation of the quality of Gastrodia elata Bl. by HPLC-DAD/MS].

The chromatographic fingerprint of Gastrodia elata Bl. (Tianma) was developed to compare the quality of Tianma samples from different habitats and processing methods. The above analysis method was established by HPLC-DAD technique.

[Quality analysis and evaluation of Rhizoma Coptidis under different cultivation conditions].

Aim: To develop methods for the fingerprint analysis of Rhizoma Coptidis and the determination of berberine, palmatine and jatrorrhizine in Rhizoma Coptidis, and analyze the contents of these three alkaloids in Rhizoma Coptidis under different cultivation conditions, from different areas and processed with different methods.

Methods: Two methods (HPLC-UV and HPLC-MS) have been developed and used in fingerprint analysis of Rhizoma Coptidis. An HPLC method was used to determine the contents of three alkaloids.

[Variety of differential display on gene and corresponding gene's clone following corneal alkali burns in rats].

Objective: To study the condition of differential gene caused after corneal alkali burns in rats and clarify the molecular biological foundation of corneal denatured protein.

Methods: The animals were sacrificed on the 3rd day and the 2nd week after alkali burns. Total RNA was isolated from the excised corneas and then reverse-transcribed into cDNA.

[Research development of Coptis chinensis].

To summarize the research and application of ecological planting technique for Coptis chinensis, and describe the recent development of its chemical components, pharmacological effects and clinical applications.

[Technique of storing Coptis chinensis seeds in damp sand under shed and fine cultivation of seedling].

Objective: To develop technique of storing Coptis chinensis seeds in damp sand under shed and fine cultivation of seedling and to enhance the cultivation seedling rate from seeds of Coptis chinensis.

Methods: With the old technique of seedling in woods as control, we screened the new method for storing seeds and cultivating seedlings.

Results: Seeds were picked up at May and stored in damp sand under shed until November, and then planted with technique of fine cultivation of seedling.

[Ecological technique of cultivating Coptis chinensis in woods and co-cultivating it with maize].

Objective: To establish ecologically new modes of cultivating Coptis chinensis in woods and co-cultivating it with maize.

Methods: Based on the experience obtained from plot comparative test and production test, we used application-oriented research methods, and established new Coptis chinensis cultivation techniques that protect the natural environment.

Results: Coptis chinensis was harvested 6 years after cultivation.

[Application and prospect on the researches of ecological cultivation of Coptis chinensis].

Traditional cultivation of Coptis chinensis was carried out under shield by disafforestation, which has been used for over 300 years and lead to the severe destruction of natural environment. Several ecological modes for cultivation of Coptis chinensis have been developed, which increase the yields of Coptis chinensis, protect the resources of forest, and obtain economic and ecologic benefit.

[The study of FK506 eye drops preventing and treating immune rejection on limbal allograft transplantation].

Objective: To evaluate the general cause of limbal allograft transplantation rejection and the effects of the immunosuppressant FK506.

Methods: Limbal deficiency models were established in 48 New Zealand rabbits, which were randomized into three groups of 16 rabbits each. Limbal allograft transplantation was performed one month later.