Publications by authors named "Jin-Shun Cai"
Producing aflatoxin-detoxifizyme (ADTZ) in pigs to control the AFT contamination of pig feed is a new research strategy by transgenic technology. In this study, transgenic pigs specifically expressing ADTZ gene in the parotid gland were successfully produced by somatic cell nuclear transfer technology. The ADTZ activity in saliva of 6 transgenic pigs was found to be 7.
View Article and Find Full Text PDFβ-Glucan is the predominant anti-nutritional factors in monogastric animal feed. Although β-glucanase supplementation in diet can help to eliminate the adverse effects, enzyme stability is substantially modified during the feed manufacturing process. To determine whether the expression of endogenous β-glucanase gene (GLU) in vivo can improve digestibility of dietary β-glucan and absorption of nutrients, we successfully produced transgenic pigs via nuclear transfer which express the GLU from Paenibacillus polymyxa CP7 in the parotid gland.
View Article and Find Full Text PDFArticle Synopsis
- A gene called aflatoxin-detoxifizyme (ADTZ) from the fungus Armillariella tabescens was inserted into a specific vector for expressing it in the parotid glands of mice, creating a transgenic mouse model.
- Six transgenic mice were successfully generated, showing ADTZ expression specifically in their parotid glands and a detectable activity of 3.72 U/mL in their saliva.
- Feeding these mice a diet with aflatoxin B1 revealed that ADTZ helped significantly improve certain blood protein levels and reduced harmful aflatoxin residues in their serum and liver, demonstrating its protective effects.