Publications by authors named "Jin-Ru Lin"
Article Synopsis
- Pfu DNA polymerase is crucial for PCR experiments, but producing it in high quantities and purity is challenging; this study presents a method to effectively express this enzyme.
- The researchers modified the Pfu DNA polymerase gene codon usage based on the enhanced green fluorescence protein, resulting in the recombinant version named rPfu, which was expressed in E. coli and yielded high levels of soluble protein.
- The purified rPfu demonstrated strong bioactivity, achieving over 88,000 units per 50 mL E. coli culture, and can amplify DNA fragments of at least 5.5 kb, suggesting that this codon usage strategy could benefit the production of other recombinant proteins.
Lithium diaqua-magnesium catena-borodiphosphate(V) monohydrate, LiMg(H(2)O)(2)[BP(2)O(8)]·H(2)O, at 173 K.
Acta Crystallogr Sect E Struct Rep Online
May 2008
The crystal structure of LiMg(H(2)O)(2)[BP(2)O(8)]·H(2)O consists of tubular structural units, built from tetra-hedral (∞) (1){[BP(2)O(8)](3-)} borophosphate ribbons and (LiO(4))(n) helices running along [001], which are inter-connected by MgO(4)(H(2)O)(2) octa-hedra, forming a three-dimensional network structure with one-dimensional channels along [001] in which the water mol-ecules are located. The water mol-ecule in the channel is significantly displaced by up to 0.3 Å from the special position 6b (.
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