Effects of Chinese medicine herbal residues on antibiotic resistance genes and the bacterial community in chicken manure composting.

The use of livestock manure is an important way for antibiotic resistance genes (ARGs) to enter the environment, and composting is an effective method for removing ARGs from livestock manure. In this study, different volume ratios of Chinese medicinal herbal residues (CMHRs) were added to laboratory-scale chicken manure composting to evaluate their effects, if any, on the behavior of ARGs, mobile genetic elements (MGEs), and the bacterial community. At the end of the composting period, the composition of the microbial community changed.

Efficacy of clomifene citrate for the treatment of patients with polycystic ovary syndrome: A protocol of systematic review.

Background: This study aims to assess the efficacy and safety of clomifene citrate (CC) for the treatment of patients with polycystic ovary syndrome (PCOS).

Methods: In this study, we will comprehensively search MEDLINE, EMBASE, The Cochrane Library, Web of Science, CINAHL, ACMD, PsycINFO, and China National Knowledge Infrastructure for original articles published from their inceptions to the January 1, 2020 without language restrictions. All studies will undergo relevance and a design selecting process.

[Effect of Co-composting of Chicken Manure with Chinese Medicinal Herbal Residues on Antibiotic Resistance Genes].

Livestock manure is an important pathway by which antibiotic resistance genes (ARGs) enter the environment. To reduce the occurrence of antibiotic resistance genes in manures, we studied the variations of ARGs and mobile gene elements (MGEs) during the 46-day co-composting of chicken manure with Chinese medicinal herbal residues. The abundance of 100 ARGs and 5 MGEs were measured by Real-Time Quantitative PCR with 0, 3, 7, 14, 28, and 46 d co-composting.

Serum soluble TWEAK levels in severe traumatic brain injury and its prognostic significance.

Background: Severe traumatic brain injury (sTBI) is characterized by a high mortality. Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) participates in inflammation. We determined serum soluble TWEAK (sTWEAK) levels with respect to its prognostic ability.

Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway.

Electroacupuncture (EA) has anti-oxidative and anti-inflammatory actions, but whether the neuroprotective effect of EA against cerebral ischemia-reperfusion (I/R) injury involves modulation of the extracellular regulated kinase 1/2 (ERK1/2) signaling pathway is unclear. Middle cerebral artery occlusion (MCAO) was performed in Sprague-Dawley rats for 2 hours followed by reperfusion for 24 hours. A 30-minute period of EA stimulation was applied to both Baihui (DU20) and Dazhui (DU14) acupoints in each rat (10 mm EA penetration depth, continuous wave with a frequency of 3 Hz, and a current intensity of 1-3 mA) when reperfusion was initiated.

[The use and establishment of modified acute physiology and chronic health evaluation II score in emergency patients].

Objective: To simplify the acute physiology and chronic health evaluation II (APACHE II) score to make it more suitable for emergency patients.

Methods: Medical records of all the dead patients in the intensive care unit (ICU) from January 2003 to October 2005 were retrospectively analyzed. Those dead patients whose clinical data were completed for study were chosen as the experimental group (153 cases).

Design, synthesis, and fungicidal activity of macrolactones and macrolactams with a sulfonamide side chain.

Four series of novel macrolactones and macrolactams12-alkylsulfonamido-1,15-pentadecanlactones ( 5), 12-alkylsulfonamido-15-methyl-1,15-pentadecanlactones ( 6), 12-alkylsulfonamido-1,15-pentadecanlactams ( 7), and N-(alkylsulfonamidoethyl)-1,12-dodecanlactams ( 8)were designed and synthesized from readily available 2-nitrocyclododecanone or cyclododecanone. Their structures were confirmed by (1)H NMR, IR, and elemental analysis. The bioassay showed that these compounds displayed fair to excellent fungicidal activity against Rhizoctonia solani Kuhn and have a gradual increase of fungicidal activity in the order of 6, 7, 8, and 5.

Synthesis and fungicidal activity of macrolactams and macrolactones with an oxime ether side chain.

Three series of novel macrolactams and macrolactones--12-alkoxyimino-tetradecanlactam, 12-alkoxyiminopentadecanlactam, and 12-alkoxyiminodecanlactone derivatives (7A, 7B, and 7C)--were synthesized from corresponding 12-oxomacrolactams and 12-oxomacrolactone. Their structures were confirmed by 1H NMR and elemental analysis. The Z and E isomers of 7A and 7B were separated, and their configurations were determined by 1H NMR.

[Etiological study on an outbreak of acute respiratory infection in children in Beijing].

Objective: An outbreak of acute respiratory infections in children occurred in Beijing from November to December, 2002. To investigate the etiological agents of affected children who were in day care centers and primary schools.

Methods: Throat swab specimens were collected from one primary school children with acute respiratory infections visiting one outpatient department.