Retraction note: Clinical analysis of colistin sulfate in the treatment of pneumonia caused by carbapenem-resistant Gram-negative bacteria.

[This retracts the article on p. 2173 in vol. 12, PMID: 38808336.

Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness.

Background: To successfully replicate within the host cell, Toxoplasma gondii employs several mechanisms to overcome the host cell defenses and mitigate the harmful effects of the free radicals resulting from its own metabolic processes using effectors such as thioredoxin proteins. In this study, we characterize the location and functions of a newly identified thioredoxin in T. gondii, which was named Trx4.

2023: A year of accomplishments for the 13 Science Citation Index Expanded- and Emerging Sources Citation Index-indexed journals.

In 2023, Baishideng Publishing Group () routinely published 47 open-access journals, including 46 English-language journals and 1 Chinese-language journal. Our successes were accomplished through the collective dedicated efforts of staffs, Editorial Board Members, and Peer Reviewers. Among these 47 journals, 7 are included in the Science Citation Index Expanded (SCIE) and 6 in the Emerging Sources Citation Index (ESCI).

The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony.

Sexual development in Toxoplasma gondii is a multistep process that culminates in the production of oocysts, constituting approximately 50% of human infections. However, the molecular mechanisms governing sexual commitment in this parasite remain poorly understood. Here, we demonstrate that the transcription factors AP2XI-2 and AP2XII-1 act as negative regulators, suppressing merozoite-primed pre-sexual commitment during asexual development.

The Toxoplasma protein phosphatase 6 catalytic subunit (TgPP6C) is essential for cell cycle progression and virulence.

Protein phosphatases are post-translational regulators of Toxoplasma gondii proliferation, tachyzoite-bradyzoite differentiation and pathogenesis. Here, we identify the putative protein phosphatase 6 (TgPP6) subunits of T. gondii and elucidate their role in the parasite lytic cycle.

A newly characterized dense granule protein (GRA76) is important for the growth and virulence of Toxoplasma gondii.

Pathogenicity of the zoonotic pathogen Toxoplasma gondii largely depends on the secretion of effector proteins into the extracellular milieu and host cell cytosol, including the dense granule proteins (GRAs). The protein-encoding gene TGME49_299780 was previously identified as a contributor to parasite fitness. However, its involvement in parasite growth, virulence and infectivity in vitro and in vivo remains unknown.

Role of SNAP25 on the occurrence and development of eosinophilic gastritis.

Eosinophilic gastritis is characterized by gastrointestinal symptoms accompanied by peripheral eosinophilia. This study aims to explore the association between eosinophilic gastritis and Synaptosome Associated Protein 25 (SNAP25), and provide a new direction for the diagnosis and treatment of eosinophilic gastritis. GSE54043 was downloaded from the gene expression omnibus database.

The antioxidant protein glutaredoxin 1 is essential for oxidative stress response and pathogenicity of Toxoplasma gondii.

Glutaredoxins (Grxs) are ubiquitous antioxidant proteins involved in many molecular processes to protect cells against oxidative damage. Here, we study the roles of Grxs in the pathogenicity of Toxoplasma gondii. We show that Grxs are localized in the mitochondria (Grx1), cytoplasm (Grx2), and apicoplast (Grx3, Grx4), while Grx5 had an undetectable level of expression.

Functional Characterization of 15 Novel Dense Granule Proteins in Toxoplasma gondii Using the CRISPR-Cas9 System.

The analysis of the subcellular localization and function of dense granule proteins (GRAs) is of central importance for the understanding of host-parasite interaction and pathogenesis of Toxoplasma gondii infection. Here, we identified 15 novel GRAs and used C-terminal endogenous gene tagging to determine their localization at the intravacuolar network (IVN), parasitophorous vacuole (PV), or PV membrane (PVM) in the tachyzoites and at the periphery of the bradyzoites-containing cysts. The functions of the 15 genes were examined in type I RH strain and 5 of these genes were also evaluated in the cyst-forming type II Pru strain.

Integrity of the editing and publishing process is the basis for improving an academic journal's Impact Factor.

Background: Journal Impact Factor™ (JIF) is often used to evaluate the relative reputation and quality of academic journals in their respective fields, and can greatly influence the quality and scope of subsequent manuscript submissions. Therefore, many if not all academic journals are interested in increasing their JIF to improve their academic impact.

Aim: To determine the importance of the integrity of the editorial and publication process in improving the academic influence of academic journals and the JIF of academic journals.

The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii.

Phenotypic switching between tachyzoite and bradyzoite is the fundamental mechanism underpinning the pathogenicity and adaptability of the protozoan parasite Toxoplasma gondii. Although accumulation of cytoplasmic starch granules is a hallmark of the quiescent bradyzoite stage, the regulatory factors and mechanisms contributing to amylopectin storage in bradyzoites are incompletely known. Here, we show that T.

Effect of deleting four Toxoplasma gondii calcium-binding EGF domain-containing proteins on parasite replication and virulence.

Several calcium-binding proteins including calcium-dependent protein kinases play important roles in several facets of the intracellular infection cycle of the apicomplexan protozoan parasite Toxoplasma gondii. However, the role of the calcium-binding epidermal growth factor (EGF) domain-containing proteins (CBDPs) remains poorly understood. In this study, we examined the functions of four CBDP genes in T.

Baishideng's database announces the first of 101 core journals and a list of high-quality academic journals in gastroenterology and hepatology.

After three rounds of rigorous evaluation of core journals in gastroenterology and hepatology conducted by the () editorial team of Baishideng Publishing Group (Baishideng), the database of Baishideng officially released the 2022 (2022 ) of 101 core journals in gastroenterology and hepatology, for the first time. The list of 101 core journals can be found at: https://www.referencecitationanalysis.

Baishideng's database announces the first of 104 core journals and a list of high-quality academic journals in orthopedics.

After three rounds of rigorous evaluation of core journals in orthopedics conducted by the () editorial team of Baishideng Publishing Group (Baishideng), the database of Baishideng officially released the 2022 (2022 ) of 104 core journals and a list of high-quality academic journals in orthopedics, for the first time on August 9, 2022. The list of 104 core journals can be found at: https://www.referencecitationanalysis.

Baishideng's database announces the first of multidisciplinary scholars.

After three rounds of rigorous evaluation of registered scholars conducted by the () editorial team of Baishideng Publishing Group (Baishideng), the database of Baishideng officially released the 2022 (2022 ) of 632 scholars from 74 countries/territories in 98 research categories, for the first time. The list of 632 scholars can be found at: https://www.referencecitationanalysis.

Functional Characterization of 17 Protein Serine/Threonine Phosphatases in Using CRISPR-Cas9 System.

Protein serine/threonine phosphatases (PSPs), found in various plants and protozoa, are involved in the regulation of various biological processes. However, very little is known about the role of PSPs in the pathogenicity of the apicomplexan protozoan . Herein, the subcellular localization of 17 PSPs (PP5, PP7, EFPP, SLP, PPM3F, PPM4, PPM5A, PPM5B, PPM6, PPM8, PPM9, PPM12, PPM14, PPM18, CTD1, CTD2, and CTD3) was examined by 6× HA tagging of endogenous genes in C-terminal.

Meeting report of the chief editorial board meeting for 2021.

The 2021 online editorial board meeting of the () was held on November 9, 2021. Jin-Lei Wang, General Manager on behalf of the Baishideng Publishing Group, and Professor Islam, one of the Editors-in-Chiefs (EiCs) of the , organized the meeting. Three EiCs and 18 Baishideng Publishing Group staff attended the meeting.

Functional Characterization of Two Thioredoxin Proteins of Using the CRISPR-Cas9 System.

Toxoplasmosis caused by infection with is an important parasitic zoonosis with a worldwide distribution. In this study, we examined the functions of two thioredoxins (namely CTrp26 and CTrx1) of tachyzoites by generation of HA tag strains or gene deficient parasites in Type I RH strain (ToxoDB#10). Immunofluorescence analysis (IFA) was used to investigate the subcellular localization of the thioredoxins (Trxs).

Effect of deletion of gra17 and gra23 genes on the growth, virulence, and immunogenicity of type II Toxoplasma gondii.

The protozoan parasite Toxoplasma gondii secretes a number of dense granule proteins (GRAs) from the dense granule organelle to manipulate the host cell. Two of these effector proteins (GRA17 and GRA23) are involved in the trafficking of molecules between the parasitophorous vacuole (PV) and the host cell cytoplasm. However, their roles in establishing chronic infection remain obscured.

iTRAQ-based Quantitative Proteomics Analysis Identifies Host Pathways Modulated during Infection in Swine.

is a leading cause of foodborne illness and consumption of undercooked pig meat is a major risk factor for acquiring toxoplasmosis, which causes a substantial burden on society. Here, we used isobaric tags for relative and absolute quantification (iTRAQ) labelling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify cellular proteins and pathways altered during infection in pigs. We also used parallel reaction monitoring-based LC-MS/MS to verify the levels of protein expression of infected spleens and mesenteric lymph nodes (MLNs).

RHΔΔ Strain of Elicits Protective Immunity Against Acute, Chronic and Congenital Toxoplasmosis in Mice.

In the present study, a dense granule protein 17 ( and novel putative transporter () double deletion mutant of RH strain was engineered. The protective efficacy of vaccination using RHΔΔ tachyzoites against acute, chronic, and congenital toxoplasmosis was studied in a mouse model. Immunization using RHΔΔ induced a strong humoral and cellular response, as indicated by the increased levels of anti- specific IgG, interleukin 2 (IL-2), IL-10, IL-12, and interferon-gamma (IFN-γ).