Expression Levels of miR-181 Family Members in Oral Biofluids as Biomarkers for Periodontitis Severity.

This study aimed to assess the diagnostic potential of microRNA-181 (miR-181) family members in oral biofluids, namely saliva and gingival crevicular fluid (GCF), as biomarkers for periodontitis severity. A cohort of 150 patients with periodontitis, including 82 with mild to moderate and 68 with advanced periodontitis, along with 90 healthy controls, were recruited. Analysis of miR-181 family expression using quantitative real-time polymerase chain reaction (qRT-PCR) revealed differential expression levels in oral biofluids among the study groups.

Differential Expressions of ADAM28 and ADAMTSL3 in Gingival Tissue of Patients with Periodontitis.

The modulation of gene expression via DNA methylation modifications is relevant to the pathogenesis of periodontitis. This study aimed at identifying novel biomarkers in gingival tissues from periodontitis by integrally analyzing methylation profiles and gene expression data. Differential gene expressions (DGEs) of dataset GSE106090 were obtained from the Gene Expression Omnibus (GEO) database for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.