Doped nanomaterial facilitates 3D printing target plate for rapid detection of alkaloids in laser desorption/ionization mass spectrometry.

This work aims to rapidly detect toxic alkaloids in traditional Chinese medicines (TCM) using laser desorption ionization mass spectrometry (LDI-MS). We systematically investigated twelve nanomaterials (NMs) as matrices and found that MoS and defect-rich-WO (D-WO) were the best NMs for alkaloid detection. MoS and D-WO can be used directly as matrices dipped onto conventional ground steel target plates.

[Degradation of -HCH in Soil Washing Solutions with nZVI and CaO].

Nano zerovalent iron (nZVI) and CaO were used to construct a heterogeneous Fenton-like system to degrade -hexachlorocyclohexane (-HCH) in soil solution, and the degradation efficiency and mechanism were investigated. The results showed that nZVI/CaO can degrade -HCH in an extensive pH range. When the dosage of nZVI and CaO was 1 g·L and pH was 5, the degradation rate of -HCH reached 93.

Mass Spectrometry Genotyping of Human Papillomavirus Based on High-Efficiency Selective Enrichment of Nanoparticles.

This work developed a novel spermine-modified nanodiamonds (SP-NDs)-based method to selectively enrich oligonucleotides for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of human papillomavirus (HPV) virus. Our results showed that SP-NDs can effectively extract and enrich DNA oligonucleotides from sodium dodecyl sulfonate and urea solution. In addition, SP-NDs can also selectively extract oligonucleotides from enzymes digestion products of polymerase chain reaction-restriction fragment mass polymorphism (PCR-RFMP) of HPV virus.

[Effects of intracerebroventricular microinjection of L-arginine on exercise capacity and expression of nitric oxide in rat hypothalamus].

Objective: To explore the relationship between nitric oxide (NO) in central nervous system and exercise-induced fatigue stress and to study the effect of L-arginine (L-Arg), as a substrate of nitric oxide, on the exercise capacity and NO content in the exhausted rat brain and blood.

Methods: Through an implanted cannula, the normal saline or L-Arg was microinjected into rat's intracerebroventrical for consecutive four days. Then an acute exhaustive model (on the speed of 18 m/min, an inclination of 5 degrees) was established with animal treadmill.