GC-MS/MS Method for Determination of Polycyclic Aromatic Hydrocarbons in Herbal Medicines.

Polycyclic aromatic hydrocarbons (PAHs) are hydrophobic organic contaminants that have a highly carcinogenic and mutagenic nature. This study aimed to develop and validate a sensitive analytical method to determine 8 PAHs in 51 herbal medicines (HMs) using gas chromatography (GC)-tandem mass spectrometry (MS/MS). Liquid--liquid extraction and florisil SPE cartridge purification were basically adopted for pretreatment.

Growth factor expression with different wound treatments after laser resurfacing.

Background: Many studies have demonstrated that the specific method of wound dressing used may affect the healing process. However, the effect of the method of wound dressings on the expression of growth factors is not well documented.

Objective: The aim of this study was to evaluate the effects of different methods of treatment on the healing process and the expression of growth factors (epidermal growth factor, basic fibroblast growth factor, transforming growth factor-beta(2) [TGF-beta(2)], platelet-derived growth factor-A, and platelet-derived growth factor-B) by histologic study, immunohistochemistry, and reverse transcription-polymerase chain reaction.