Anti-proliferative and anti-invasive effects of garcinol from on gallbladder carcinoma cells.

Garcinol, a natural histone acetyltransferase inhibitor, has been reported to exhibit significant anti-proliferative activity in various cancer cell types. However, no information is available about the anti-cancer effects of garcinol on gallbladder carcinoma cells (GBC). In this study, GBC cells (GBC-SD and NOZ) were treated by garcinol and subjected to Cell Counting Kit-8 (CCK-8), and GBC-SD cells were selected for further transwell chamber assay, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis.

Fu's subcutaneous needling: possible clinical evidence of the subcutaneous connective tissue in acupuncture.

Objectives: Recently it was reported that the loose connective tissue in the subcutaneous layer rapidly responded to mechanical forces evoked by acupuncture, massage, and normal physical movements. However, there were no clinical studies to substantiate these findings so far. Fu's Subcutaneous Needling (FSN) is the innovative needling strategy acting specifically in the subcutaneous layer.