LncRNA Promotes Hepatocellular Carcinoma Migration and Invasion Via Targeting the miR-137/AKT2 Axis.

The current study aimed to investigate the effects of on the migration and invasion of hepatoma cells. The expressions of , miR-137, and were detected in hepatoma tissues and cells by performing quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The correlations among , miR-137, and were predicted by bioinformatics analysis and confirmed by dual-luciferase reporter assay, and Pearson test was performed to analyze their relevance.

Complete mitochondrial genome of the oriental armyworm Mythimna separata (Walker) (Lepidoptera: Noctuidae).

We determined the complete mitochondrial genome of the oriental armyworm Mythimna separata (Walker) (Lepidoptera: Noctuidae), which is one of the serious cereal pests in Asia and Australia. The circular genome of 15,332 bp in length contains 37 typical animal mitochondrial genes and a non-coding A+T-rich region. Its gene content and order are typical of lepidopteran mitochondrial genomes described to date.

Complete mitochondrial genome of the common cutworm Spodoptera litura (Lepidoptera: Noctuidae).

We determined the complete mitochondrial genome of the common cutworm Spodoptera litura (Lepidoptera: Noctuidae), which is one of the most destructive polyphagous insect pests worldwide. The genome is 15,383 bp in length (GenBank accession number: KF701043) with an A+T content of 81.08%, and contains 37 typical animal mitochondrial genes (13 protein-coding genes, 2 rRNA genes and 22 tRNA genes) with the typical arrangement found in Lepidoptera.

Polysaccharide from Phellinus linteus induces S-phase arrest in HepG2 cells by decreasing calreticulin expression and activating the P27kip1-cyclin A/D1/E-CDK2 pathway.

Ethnopharmacology Relevance: Our previous study showed that the proteoglycan P1 from Phellinus linteus (Mesima) exhibits significant anti-tumor activity against human hepatocellular carcinoma cells (HepG2); however, its molecular mechanism remains unknown. This study aims to provide insights into the mechanism of the anti-tumor activity of P1 against HepG2 cells.

Methods: We examined the effects of P1 on HepG2 cell proliferation in vitro and in vivo.

Proteome analysis of silkworm, Bombyx mori, larval gonads: characterization of proteins involved in sexual dimorphism and gametogenesis.

Sexual dimorphism is initialed by the components of the sex determination pathway and is most evident in gonads and germ cells. Although striking dimorphic expressions have been detected at the transcriptional level between the silkworm larval testis and the ovary, the sex-dimorphic expressions at the protein level have not yet been well characterized. The proteome of silkworm larval gonads was investigated using a shotgun-based identification.

Shotgun proteomic analysis on the embryos of silkworm Bombyx mori at the end of organogenesis.

Embryonic development of silkworm, Bombyx mori is a process of systematical expression of genes and proteins which is dominated by complex regulatory networks. To gain comprehensive insight into the molecular basis of embryonic development and its regulation mechanisms, the proteome profile of the B. mori embryos at the end of organogenesis (tubercle appearance stage, TA) was characterized using LTQ-Orbitrap mass spectrometer.

Proteomic and bioinformatic analysis on endocrine organs of domesticated silkworm, Bombyx mori L. for a comprehensive understanding of their roles and relations.

Three organs of silkworm larva endocrine system, including brain (Br), subesophageal ganglion (SG) and prothoracic glands (PG), were studied employing shotgun LC-MS/MS combined with bioinformatic analysis to comprehensively understand their roles and relations. Totally, 3430, 2683, and 3395 proteins were identified including 1885 common and 652, 253, and 790 organ-specific ones in Br, SG, and PG, respectively. Identified common-expressed proteins indicated the existence of intrinsic complex interactions among these parts of endocrine system.

Comparison of transformation efficiency of piggyBac transposon among three different silkworm Bombyx mori Strains.

The transformation rate of three different strains of silkworm Bombyx mori was compared after the introduction of enhanced green fluorescence protein (EGFP)-encoding genes into the silkworm eggs by microinjection of a mixture of piggyBac vector and helper plasmid containing a transposase-encoding sequence. Although there were no significant differences among the three strains in the percentages of fertile moths in microinjected eggs (P=0.1258), the percentages of G(0) transformed moths in fertile moths and injected eggs were both significantly different (P=0.