A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase.

We have discovered a novel chemical compound, (E)-3-(furan-2-yl)--(4-sulfamoylphenyl) acrylamide, that suppresses the enzymatic activities of SARS coronavirus helicase. To determine the inhibitory effect, ATP hydrolysis and double-stranded DNA unwinding assays were performed in the presence of various concentrations of the compound. Through these assays, we obtained IC values of 2.

Totally Laparoscopic Total Gastrectomy Versus Laparoscopically Assisted Total Gastrectomy for Gastric Cancer.

Background: This study was designed to evaluate the surgical outcomes as well as the morbidity and mortality of totally laparoscopic total gastrectomy (TLTG) compared to laparoscopically-assisted total gastrectomy (LATG), and to confirm the feasibility and safety of TLTG.

Patients And Methods: Between August 2009 and January 2014, 56 patients underwent laparoscopic total gastrectomy for gastric cancer. Among them, 27 underwent TLTG using a linear stapler and 29 underwent LATG using a circular stapler for esophagojejunostomy.

Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.

A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.

Use of video-assisted thoracoscopic surgery to retrieve a broken guidewire.

Subclavian venous catheterization was once widely used for volume resuscitation, emergency venous access, chemotherapy, parenteral nutrition, and hemodialysis. However, its use has drastically reduced recently because of life-threatening complications such as hemothorax, pneumothorax. In this case, a patient admitted for a scheduled operation underwent right subclavian venous catheterization for preoperative, intraoperative, and postoperative volume resuscitation and parenteral nutrition.

Pinch-off syndrome.

Subclavian venous catheterization was previously frequently performed, but because of life-threatening complications such as hemothorax, pneumothorax, mediastinal hematoma, and myocardial injury, its use has become less common. However, this practice has some advantages in patient mobility, secured dressing, and rapidity and adequacy of vascular access. In some situations where patient comfort is an especially important consideration, such as with totally implantable venous port insertion for chemotherapy, the subclavian route can be a good choice if an experienced and well-trained faculty is available.