Construction of an alternative glycerol-utilization pathway for improved β-carotene production in Escherichia coli.

Glycerol, which is an inevitable by-product of biodiesel production, is an ideal carbon source for the production of carotenoids due to its low price, good availability and chemically reduced status, which results in a low requirement for additional reducing equivalents. In this study, an alternative carbon-utilization pathway was constructed in Escherichia coli to enable more efficient β-carotene production from glycerol. An aldehyde reductase gene (alrd) and an aldehyde dehydrogenase gene (aldH) from Ralstonia eutropha H16 were integrated into the E.

[Construction and targeting study of eukaryotic expression vector modulated by a macrophage-specific promoter].

Aim: To construct a macrophage-specific eukaryotic expression vector and to investigate its expressing specificity.

Methods: Macrophage-specific promoter was synthesized using PCR, and substituted the CMV promoter of eukaryotic expression vector pEGFP-N1 to construct a recombinant vector (pSP-GFP), which were cotransfected with pERFP-N1 vector into different cell lines. The green fluorescent protein (GFP) and red fluorescent protein (RFP) was observed by fluorescence microscopy, and the target specificity of macrophage-specific promoter was judged by comparison of expressed level of GFP and RFP in various cell lines.

2-(4-Dimethyl-amino-2-hydroxy-benzoyl)benzoic acid methanol solvate.

In the title compound, C(16)H(15)NO(4)·CH(4)O, the dihedral angle between the benzene rings is 75.21 (5)°. The structure is stabilized by an intra-molecular O-H⋯O inter-action [O⋯O = 2.

A global assembly of cotton ESTs.

Approximately 185,000 Gossypium EST sequences comprising >94,800,000 nucleotides were amassed from 30 cDNA libraries constructed from a variety of tissues and organs under a range of conditions, including drought stress and pathogen challenges. These libraries were derived from allopolyploid cotton (Gossypium hirsutum; A(T) and D(T) genomes) as well as its two diploid progenitors, Gossypium arboreum (A genome) and Gossypium raimondii (D genome). ESTs were assembled using the Program for Assembling and Viewing ESTs (PAVE), resulting in 22,030 contigs and 29,077 singletons (51,107 unigenes).