Bioengineered Polyhydroxyalkanoates as Immobilized Enzyme Scaffolds for Industrial Applications.

Enzymes function as biocatalysts and are extensively exploited in industrial applications. Immobilization of enzymes using support materials has been shown to improve enzyme properties, including stability and functionality in extreme conditions and recyclability in biocatalytic processing. This review focuses on the recent advances utilizing the design space of self-assembled polyhydroxyalkanoate (PHA) particles as biocatalyst immobilization scaffolds.

Covalent Functionalization of Bioengineered Polyhydroxyalkanoate Spheres Directed by Specific Protein-Protein Interactions.

Bioengineered polyhydroxyalkanoate (PHA) spheres assembled in engineered bacteria are showing promising potential in protein immobilization for high-value applications. Here, we have designed innovative streamlined approaches to add functional proteins from complex mixtures (e.g.

Design of Modular Polyhydroxyalkanoate Scaffolds for Protein Immobilization by Directed Ligation.

In vivo-assembled polyhydroxyalkanoate (PHA) particles have been successfully bioengineered to display foreign protein functions toward high-value applications in medicine and industry. To further expand the design space of PHA particles toward immobilization of various functional proteins, we developed a tunable modular protein immobilization method implementing the SpyCatcher/SpyTag chemistry. We successfully displayed the SpyCatcher protein using translational fusion with the Ralstonia eutropha PHA synthase (PhaC).