The search for optimal nocturnal diurnal heart rate Index targets in ICU patients: a retrospective observational study from large ICU database.

Background: Circadian rhythms play a crucial role in cardiovascular health, with the nocturnal diurnal heart rate index (NDHRI) reflecting significant circadian variations. However, the optimal NDHRI target in Intensive Care Unit (ICU) patients remains undefined. This study aims to establish an evidence-based NDHRI target range and assess its association with mortality.

Association of H3K9me3 with breast cancer prognosis by estrogen receptor status.

Background: Cellular experiments revealed that a decreased histone H3 lysine 9 trimethylation (H3K9me3) level was associated with the upregulation of oncogenes in breast cancer cells. Moreover, the role of H3K9me3 in breast cancer was closely associated with estrogen receptor (ER) status. Therefore, we aimed to examine the prognostic value of H3K9me3 on breast cancer by ER status.

Increasing plasmid-based DNA vaccine construct (16 kb pSVK-HBVA) production in XL10-Gold through optimization of media component.

At present, there are production processes to produce protein by () fermentation. Research on the design and optimization of the plasmid fermentation medium, however, is less advanced. The fermentation medium that is optimized for plasmid DNA production is different from the medium that is optimized for protein production.

Design, expression, and characterization of a novel dendritic cell-targeted proteins.

In vivo approaches to inducing an effective immune response focus on targeted antigen (Ag) delivery to dendritic cells (DCs). In this study, we developed a new method of targeting plasmid DNA and/or the antigen (Ag)-antibody (Ab) complex to DCs via the DC receptor DEC-205, also known as cluster of differentiation CD205. We cloned and expressed a recombinant protein composed of mouse DEC-205-specific single-chain fragment variable region (mDEC-205-scFv), the streptococcal protein G (SPG) IgG-binding domain and cationic peptide (CP), which named mDEC205-scFv-SPG-CP (msSC).

Enhancement of antitumor immunity using a DNA-based replicon vaccine derived from Semliki Forest virus.

A DNA-based replicon vaccine derived from Semliki Forest virus, PSVK-shFcG-GM/B7.1 (Fig. 1a) was designed for tumor immunotherapy as previously constructed.

[Construction and expression of the efficient recombinant plasmid pEE14.1-IFN-α expressing human IFN-α].

Aim: To construct the eukaryotic expression plasmid pEE14.1-IFN-α expressing human IFN-α gene, and to detect the expression of the plasmid in eukaryotic cells.

Methods: The human IFN-α gene amplified by PCR and was linked into pCI-GPI, then inserted into the eukaryotic expression vector pEE14.

[Construction of a replicative anti-tumor DNA vaccine PSCK-2PFcGB and its expression in vivo and in vitro].

Objective: To construct a replicative anti-tumor DNA vaccine PSCK-2PFcGB based on Semliki Forest Virus (SFV) replicon vector and observe its expression in vivo and in vitro.

Methods: The plasmid pVAX1-2PFcGB was digested with Nhe I, and the digestion product was blunted prior to further digestion with BssH II to obtain the fragment 2PFcGB, a fusion gene containing the multitarget complex antigen 2PAG encoding both the most cytotoxic T lymphocyte epitopes of human survivin and chorionic gonadotropin β chain-CTP37 of human and monkey. The 2PFcGB fragment was inserted into the PSCK vector digested by Sma I.

[Construction of eukaryotic expression vector of human hCGbeta and establishment of stably transfected B16 cell line].

Aim: To construct the eukaryotic expression vector of human hCGbeta and stably transfect B16 cell line with it.

Methods: The full length of hCGbeta cDNA fragment was amplified by PCR and inserted into eukaryotic expression vector pIRES-neo, added the restriction enzyme position and 6xHis tag. After identification of restriction digestion and PCR, The recombinant plasmid pIRES-neo-hCGbeta-(His)6; was obtained.