Gas-Phase Preparation of 1-Germavinylidene (HCGe; XA), the Isovalent Counterpart of Vinylidene (HCC; XA), via Non-adiabatic Dynamics through the Elementary Reaction of Ground State Atomic Carbon (C; P) with Germane (GeH; XA).

1-Germavinylidene (HCGe; XA), the germanium analogue of vinylidene (HCC; XA), was prepared via a directed gas-phase synthesis through the bimolecular reaction of ground state atomic carbon (C; P) with germane (GeH; XA) under single-collision conditions. The reaction commences with the barrierless insertion of carbon into the Ge-H bond followed by intersystem crossing from the triplet to singlet surface and migration of atomic hydrogen to germylene (HGeCH), which predominantly decomposes via molecular hydrogen loss to 1-germavinylidene (HCGe; XA). Therefore, the replacement of a single carbon atom in the acetylene-vinylidene system by germanium critically impacts the chemical bonding, molecular structure, and thermodynamic stability of the carbene-type structures favoring 1-germavinylidene (HCGe) over germyne (HGeCH) by 160 kJ mol.

Association between Maternal Drug Use and Cytochrome P450 Genetic Polymorphisms and the Risk of Congenital Heart Defects in Offspring.

Objective: This study aimed to assess the associations between maternal drug use, cytochrome P450 ( ) genetic polymorphisms, and their interactions with the risk of congenital heart defects (CHDs) in offspring.

Methods: A case-control study involving 569 mothers of CHD cases and 652 controls was conducted from November 2017 to January 2020.

Results: After adjusting for potential confounding factors, the results show that mothers who used ovulatory drugs (adjusted odds ratio [a ] = 2.

[Immune Modulatory Effect of Outer Membrane Vesicles Derived from on Mouse Bone Marrow-Derived Dendritic Cells].

Objective: To study the effect of outer membrane vesicles (OMVs) derived from (ST) on the ultrastructural features and immune function of dendritic cells (DC).

Methods: Mice bone marrow cells were collected aseptically, and myeloid DC were generated by the combined induction and amplification with recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and recombinant mouse interleukin-4 (rm IL-4). Cell morphology was observed under inverted phase contrast microscope and the phenotype was identified with flow cytometry.

Curcuma's extraction attenuates propranolol-induced psoriasis like in mice by inhibition of keratin, proliferating cell nuclear antigen and toll-like receptor expression.

Curcuma was the dried rhizomes of Curcuma kwangsiensis S.G. Lee et C.

[Association of maternal diabetes mellitus and UCP2 gene polymorphisms with congenital heart disease in offspring: a case-control study].

Objective: To study the association of maternal diabetes mellitus (DM), uncoupling protein 2 (UCP2) gene polymorphisms, and their interaction with the risk of congenital heart disease (CHD) in offspring.

Methods: A hospital-based case-control study was conducted. A total of 464 mothers of children with CHD alone who were diagnosed in Hunan Children's Hospital from March 2018 to August 2019 were enrolled as the case group.

Nuclear factor-κB in rheumatoid arthritis.

NF-κB (nuclear factor kappa light chain enhancer of activated B cells) signaling pathway is involved in the occurrence and development of various kinds of inflammation, including rheumatoid arthritis (RA). In this paper, the relationship between the activation or inhibition of NF-κB and the pathogenesis of RA is discussed. It is found that activated NF-κB operates a dual-aspect effect which can either promote or suppress inflammation.

[Research on Immunomodulatory Effects of L-form on Mouse Splenic Dendritic Cells].

Objective: To study the effect of (LM) infection on dendritic cell (DC) immune function, and to compare the difference of immune activation ability on DC cell between bacterial form (WT) and L-form.

Methods: C57BL/6 mice were randomly divided into 3 groups and infected intravenously with LM WT, LM L-form or PBS respectively. The ultrastructural characteristics of splenic DC were observed by transmission electron microscopy (TEM).

In vitro and in vivo metabolic profiles of fasiglifam using ultrahigh-performance liquid chromatography combined with Q-Exactive Orbitrap tandem mass spectrometry.

Rationale: Fasiglifam is an orally available and selective partial agonist of hGPR40 receptor, which was unexpectedly terminated at phase III clinical trials due to its severe hepatotoxicity. To fully understand the mechanism of action of fasiglifam, it is necessary to investigate its in vitro and in vivo metabolic profiles.

Methods: For in vitro metabolism, fasiglifam was incubated with rat or human liver microsomes in the presence of β-nicotinamide adenine dinucleotide phosphate tetrasodium salt, glutathione (GSH) and uridine diphosphate glucuronic acid trisodium salt for 60 min.

Simultaneous determination of 20(S)-protopanaxadiol and its three metabolites in rat plasma by LC-MS/MS: application to their pharmacokinetic studies.

The aim of this study was to develop an LC-MS/MS method for simultaneous determination of 20(S) protopanaxadiol (PPD) and its three metabolites, PPD-glucuronide (M1), (20S,24S)-epoxy-dammarane-3,12,25-triol (M2) and (20S,24R)-epoxydammarane-3,12,25-triol (M3), in rat plasma. Precipitation with acetonitrile was employed for sample preparation and chromatographic separations were achieved on a C column. The sample was detected using triple quadrupole tandem mass spectrometer with selected reaction monitoring mode.

A validated LC-MS/MS method for the determination of senkyunolide I in dog plasma and its application to a pharmacokinetic and bioavailability studies.

Senkyunolide I is one of the major bioactive components in the herbal medicine Ligusticum chuanxiong. The aim of this study was to develop and validate a fast, simple and sensitive LC-MS/MS method for the determination of senkyunolide I in dog plasma. The plasma samples were processed with acetonitrile and separated on a Waters Acquity UPLC BEH C column (50 × 2.

Rapid and sensitive headspace gas chromatography-mass spectrometry method for the analysis of ethanol in the whole blood.

Background: For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min.

3-(5-Oxo-3-phenyl-4,5-dihydro-1H-pyrazol-1-yl)benzonitrile.

In the title compound, C(16)H(11)N(3)O, the dihedral angles between the 3-cyano-benzene and benzene planes and the 1H-pyrazol-5(4H)-one plane are 4.97 (9) and 9.91 (9)°, respectively.

[The Enhanceing effect of IL-12 on phagocytosis and killing of Mycobacterium tuberculosis by neutrophils in tuberculosis patients].

Aim: To explore the effects of IL-12 on phagocytosis and killing of Mycobacterium tuberculosis by neutrophils or polymorphonuclear cells (PMNs) in tuberculosis patients.

Methods: The fresh peripheral blood samples from TB patients and healthy adults were incubated with M.tb labeled with FITC, and the percentages of phagocytosis of M.

5-(1H-Indol-3-yl-methyl-idene)-2,2-di-methyl-1,3-dioxane-4,6-dione.

In the title compound, C(15)H(13)NO(4), the conjugated double-bond system between the two rings adopts a cis configuration and there is an intra-molecular indole-ketone C-H⋯O inter-action. The indole N-H group forms an inter-molecular hydrogen bond with a ketone O-atom acceptor, giving a chain structure along the ab direction. The O-heterocyclic ring adopts a boat conformation and makes a dihedral angle of 16.

2,2-Dimethyl-5-[(pyridin-2-yl-amino)-methyl-idene]-1,3-dioxane-4,6-dione.

In the title compound, C(12)H(12)N(2)O(4), the dihedral angle between the pyridine and enamine planes is 3.5 (3)°, while the angle between the dioxanedione (seven atoms) and enamine planes is 4.6 (3)°.

Interleukin 17-producing gamma delta T cells increased in patients with active pulmonary tuberculosis.

Although it has been known that gammadelta T cells may play an important role in the immune response to infection of Mycobacterium tuberculosis (M. tb), the mechanisms by which the gammadelta T cells participate in the innate and/or acquired immunity to tuberculosis (TB) have not been full elucidated. In the present study, 27 patients with active pulmonary TB and 16 healthy donors (HD) were performed.