Preparation and Characterization of Folate Targeting Magnetic Nanomedicine Loaded with Gambogenic Acid.

To engineer multifunctional nanomedicines for simultaneous imaging and therapy of cancer cells, we have prepared Gambogenic acid (GNA) loaded folic acid (FA) armed MNPs (FA-GNA-MNPs) to target the folate receptor (FR) positive cancer cells. The FA-GNA-MNPs have been prepared by a facile method, which have been further characterized by SEM, TEM, IR and UV-vis spectra. And the cytotoxicity of FA-GNA-MNPs to HeLa and A549 cells was assessed using the MTT assay.

[Roles of the cross talk between MAP kinases and Keap1-Nrf2-ARE signaling pathways in chronic obstructive pulmonary disease].

Chronic obstructive pulmonary disease (COPD), a common preventable and treatable disease, is characterized by airflow limitation that is usually progressive and associated with an enhanced chronic inflammatory response in the airways. Its main pathological manifestations include airway inflammation, mucus hypersecretion, oxidative stress and apoptotic epithelial cells. Recent research suggests that MAP kinases and Keap1-Nrf2-ARE signaling pathway are involved in the pathological process of inflammation and oxidative stress.

[Influence of huatanjiangqi prescription on multidrug resistance-associated protein 1 in human bronchial epithelial cells].

Objective: To investigate the effect of Huatanjiangqi prescription (Sinapis Semen, Perillae Fructus, Cynanchi Stauntonii Rhizoma et Radix, Inulae Herba, Angelicae Sinensis Radix, Honey-fried Ephedrae Herba) on multidrug resistance-associated protein 1 (MRP1) in human bronchial epithelial cells.

Methods: The human bronchial epithelial cells line 16HBE140- was used to analyze the in vitro effect of Huatanjiangqi prescription on MRP1 transport. 5-CFDA was used as a model MRP1 substrate and was measured with flow cytometry.

Ultra-high-performance liquid chromatography tandem mass spectrometry method for the determination of gambogenic acid in dog plasma and its application to a pharmacokinetic study.

A highly sensitive and rapid ultra-high-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of gambogenic acid in dog plasma. Gambogic acid was used as an internal standard (IS). After a simple liquid-liquid extraction by ethyl acetate, the analyte and internal standard were separated on an Acquity BEH C18 (100 × 2.

Allyl isothiocyanate increases MRP1 function and expression in a human bronchial epithelial cell line.

Multidrug resistance-associated protein 1 (MRP1), a member of the ATP-binding cassette (ABC) superfamily of transporters, plays an important role in normal lung physiology by protecting cells against oxidative stress and toxic xenobiotics. The present study investigates the effects of allyl isothiocyanate (AITC) on MRP1 mRNA and MRP1 protein expression and transporter activity in the immortalised human bronchial epithelial cell line 16HBE14o-. MRP1 mRNA and MRP1 protein expression in 16HBE14o- cells that were treated with allyl isothiocyanate were analysed by real-time PCR assay and Western blotting.

[Spread of rectal cancer in the distal mesorectum: a report of 46 cases].

Background & Objective: Total mesorectum excision (TME) for rectal cancer may reduce local recurrence by complete eradication of metastatic foci in the distal mesorectum. While the spread regulation of rectal cancer in the distal mesorectum and ideal length of mesorectum resection are still unclear. This study was to investigate the spread of rectal cancer in the distal mesorectum.