Water quality characteristics and reuse potential using adsorption as a post-treatment option for a full-scale hydrocyclone, coagulation, flocculation, and dissolved air flotation system.

This study established a full-scale hybrid water treatment system combining a hydrocyclone, coagulation, flocculation, and dissolved air flotation unit (HCFD) and evaluated its performance in treating anthropogenically impacted lake water. The HCFD system offered the stable and efficient treatment of fluctuating influent loadings, meeting most of the highest water reclamation quality criteria except for that of organic matter. Adsorption was subsequently examined as a post-treatment process for the HCFD effluent, which has not been examined in many previous studies.

IGF-I improves mitochondrial membrane potential during hypothermic storage of canine spermatozoa.

The aim of study was to evaluate the effects of insulin-like growth factor I (IGF-I) on canine sperm function during cooled and freeze-thaw storage. Extenders supplemented with different IGF-I concentrations (0, 100 and 200 ng/ml) were added to canine spermatozoa, and the sperm samples were stored at 4°C for 48 hr or freeze-thawed. Sperm motility, morphology, plasma-membrane integrity (PMI) and mitochondrial membrane potential (MMP) were evaluated.

Bioreactor engineering using disposable technology for enhanced production of hCTLA4Ig in transgenic rice cell cultures.

Two kinds of disposable bioreactors, air-lift disposable bioreactors (ADB) and wave disposable bioreactors (WDB) were compared with stirred-tank reactors (5-L STR). These bioreactors were successfully applied to transgenic rice cell cultures for the production of recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig). In both systems, a fed-batch culture method was used to produce hCTLA4Ig efficiently by feeding concentrated amino acids and production levels were enhanced when dissolved oxygen (DO) level was regulated at 30% using pure oxygen sparging.