Shanghai Kou Qiang Yi Xue
April 2014
Purpose: To investigate the effect of human vascular endothelial cells (HUVEC) on the migration of human periodontal ligament cells (HPDLC) and human gingival fibroblasts (HGF) through a model mimicking periodontal wound healing or regeneration.
Methods: HUVEC, HPDLC and HGF were co-cultured using a Transwell system, while HPDLC or HGF was cultured independently as control. Chemotaxis assay was performed using cell culture inserts (0.
Shanghai Kou Qiang Yi Xue
October 2013
Purpose: To investigate the effect of vascular endothelial cells on the proliferation of periodontal ligament cells and gingival fibroblasts through a model mimicking the periodontal wound healing and regeneration.
Methods: Human vascular endothelial cells, periodontal ligament cells and gingival fibroblasts were co-cultured using a Transwell system. The number of each type of cells at 2, 4, 6, 8 and 10 days after co-culture were counted using a cell counter machine, while periodontal ligament cells and gingival fibroblasts single culture were used as control.