Publications by authors named "Jiming Xiao"
Background: Somatic cell fusion is a process that transfers cytoplasmic and nuclear genes to create new germplasm resources. But our limited understanding of the physiological and molecular mechanisms that shape protoplast responses to fusion.
Method: We employed flow cytometry, cytology, proteomics, and gene expression analysis to examine the sugarcane (Saccharum spp.
Article Synopsis
- The study investigates the physiological and molecular responses of sugarcane protoplasts to enzymatic digestion, aiming to enhance protoplast regeneration for functional genomics and breeding.
- Protoplast viability exceeded 90% post-enzymolysis, but increased malondialdehyde levels indicated oxidative stress and decreased antioxidant enzyme activity.
- Proteomic analysis revealed significant changes in protein expression related to metabolic processes and oxidative stress, with upregulation of osmotic stress resistance genes and downregulation of regeneration-related genes.
Sugarcane somatic cell hybridization can break through the barrier of genetic incompatibility between distantly related species in traditional breeding. However, the molecular mechanisms of sugarcane protoplast regeneration and the conditions for protoplast preparation remain largely unknown. In this study, young sugarcane (ROC22) leaves were enzymatically digested, and the viability of protoplasts reached more than 90% after enzymatic digestion (Enzymatic combination: 2% cellulase + 0.
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