Blockade of ZMIZ1-GATA4 Axis Regulation Restores Youthfulness to Aged Cartilage.

Susceptibility to cartilage degeneration increases in an age-dependent manner and older cartilage exhibits increased catabolic factor expression leading to osteoarthritis (OA). While inhibition of cellular senescence can prevent age-related diseases, the understanding of the regulators governing cartilage senescence and the potential for senolytic intervention remains limited. Here, in vitro and in vivo results are reported, demonstrating for the first time that the transcriptional regulator, ZMIZ1, is upregulated in aged and OA cartilage, and that it acts through GATA4 to accelerate chondrocyte senescence and trigger cartilage deterioration.

The effects of the image attributes of golf star athletes supported by adult amateur golfers on sports participation desire and continuation intention.

The image attributes of sports star athletes demonstrate positive effects on sports fans' psychological factors. In the context of golf, fans are also influenced by the players they support, experiencing a range of emotions. Therefore, this study aimed to examine the effects of the image attributes of golf star athletes-supported by adult amateur golfers-on the desire for sports participation and the intention to continue.

Potential of human dental pulp stem cell-derived conditioned medium for amelo-/odontoblastic differentiation of HERS/ERM cells.

HERS/ERM cells constitute the only dental epithelial population that can be isolated from adult human teeth. Although HERS/ERM cells are the most studied dental epithelial cell source, information on their differentiation is lacking: these cells have a long induction period and low mineralization without coculture with dental mesenchymal stem cells. To characterize and develop an effective method for differentiating dental epithelial cells, we observed the epithelial‒mesenchymal interaction effects of deciduous dental pulp stem cell (dDPSC)-derived conditioned media (CM) during HERS/ERM cell differentiation.

Unexpected vulnerability of to polymyxin B under anaerobic condition.

Gram-positive exhibited higher susceptibility (>4-fold) to polymyxin B (PMB), the canonical antimicrobial peptide against Gram-negative bacteria, under anaerobic condition than aerobic condition. Anaerobically grown exhibited high vulnerability to PMB, leading to alteration of cell surface and morphology, as observed based on their high dansyl-PMB affinity (>2.9-fold), a proportion (>8.

Advanced bacteria-based biomaterials for environmental applications.

A large amount of anthropogenic CO emissions are derived from Portland cement production, contributing to global warming, which threatens human health and exposes flora and fauna to ecological imbalance. With concerns about the high maintenance and repair costs of concrete, the development of microbially induced calcium carbonate precipitation (MICP)-based self-healing concrete has been extensively examined. Bacterial carriers for microcrack healing could enhance the concrete's self-healing capacity by maintaining bacterial activity and viability.

Comparative Analysis of Stroke, Marital Intimacy, Marital Satisfaction and Divorce Intention According to the Type of Participation in Marital Leisure Sports Activities.

This study examined strokes, marital intimacy, marital satisfaction, and divorce intentions among participants who are members of a couple, based on their leisure sports participation. We conducted a questionnaire survey with 321 participants. The validity and reliability were checked, and a multivariate analysis of variance was conducted to test for differences between groups.

AGMB00912 protects weaning mice from ETEC infection and enhances gut health.

Maintaining a healthy intestinal environment, optimal epithelial barrier integrity, and balanced gut microbiota composition are essential for the growth performance of weaning pigs. We identified AGMB00912 (LA) in healthy porcine feces as having antimicrobial activity against pathogens and enhanced short-chain fatty acid (SCFA) production. Herein, we assess the protective role of LA using a weaning mouse model with enterotoxigenic (ETEC) infection.

Comparative genomic analysis of copepod humoral immunity genes with sex-biased expression in Labidocera rotunda.

Studies of innate immune system function in invertebrates have contributed significantly to our understanding of the mammalian innate immune system. However, in-depth research on innate immunity in marine invertebrates remains sparse. We generated the first de novo genome and transcriptome sequences of copepod Labidocera rotunda using Illumina paired-end data and conducted a comparative genome analysis including five crustaceans (four copepods and one branchiopod species).

Therapeutic Potential of FB091 in Alleviating Alcohol-Induced Liver Disease through Gut-Liver Axis.

Alcoholic liver disease (ALD) poses a significant global health burden, often requiring liver transplantation and resulting in fatalities. Current treatments, like corticosteroids, effectively reduce inflammation but carry significant immunosuppressive risks. This study evaluates FB091, a newly isolated probiotic strain, as a safer alternative for ALD treatment.

Delineation of the substrate recognition domain of MARCHF6 E3 ubiquitin ligase in the Ac/N-degron pathway and its regulatory role in ferroptosis.

Nα-terminal acetylation in eukaryotic proteins creates specific degradation signals (Ac/N-degrons) targeted for ubiquitin-mediated proteolysis via the Ac/N-degron pathway. Despite the identification of key components of the Ac/N-degron pathway over the past 15 years, the precise recognition domain (Ac/N domain) remains unclear. Here, we defined the Ac/N domain of the endoplasmic reticulum MARCHF6 E3 ubiquitin ligase through a systematic analysis of its cytosol-facing regions using alanine-stretch mutagenesis, chemical crosslinking-based co-immunoprecipitation-immunoblotting, and split-ubiquitin assays in human and yeast cells.

The occurrence of positive selection on BicA transporter of Microcystis aeruginosa.

The rapid growth of cyanobacteria, particularly Microcystis aeruginosa, poses a significant threat to global water security. The proliferation of toxic Microcystis aeruginosa raises concerns due to its potential harm to human health and socioeconomic impacts. Dense blooms contribute to spatiotemporal inorganic carbon depletion, promoting interest in the roles of carbon-concentrating mechanisms (CCMs) for competitive carbon uptake.

Effects of golf instructors' professional certification levels on amateur golfers' perception of instructor expertise, instructor credibility, and lesson participation intention: testing placebo and nocebo effects.

This study investigated the differences in amateur golfers' perceptions of instructor expertise, instructor credibility, and lesson participation intention depending on the golf instructor's certification level to investigate whether placebo and nocebo effects exist depending on the certification level. Accordingly, the study analyzed 153 amateur golfers with at least 1 year of playing experience, and the results were as follows: First, there was a difference in the perception of instructor expertise among amateur golfers depending on the golf instructor's certification level. Specifically, there were significant differences in perceived performance and teaching skills but no differences in personality and emphasis on basic principles.

Differentiation of Human-induced Pluripotent Stem Cell-derived Dental Stem Cells through Epithelial-Mesenchymal Interaction.

Research on tooth regeneration using human-induced pluripotent stem cells (hiPSCs) is valuable for autologous dental regeneration. Acquiring mesenchymal and epithelial cells as a resource for dental regeneration is necessary because mesenchymal-epithelial interactions play an essential role in dental development. We reported the establishment of hiPSCs-derived dental epithelial-like cell (EPI-iPSCs), but hiPSCs-derived dental mesenchymal stem cells (MSCs) have not yet been reported.

Relationship between Athletes' Big Five Model of Personality and Athletic Performance: Meta-Analysis.

Academic interest in athletic performance is ongoing. To examine the correlation between athletic performance and athletes' personality types, data extraction in line with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines was completed in October 2021, and a meta-analysis was performed using 180 data from 18 selected papers using the "meta" package version 4.8-4 of R Studio 3.

Implication of Amyloid Precursor-like Protein 2 Expression in Cutaneous Squamous Cell Carcinoma Pathogenesis.

Background/aim: Regulatory functions of amyloid precursor-like protein 2 (APLP2) expression in intracellular trafficking of major histocompatibility complex class I (MHC-I) and biological behavior of tumor cells have been reported in various types of malignancies but not in cutaneous squamous cell carcinoma (CSCC). This study aimed to investigate the role of APLP2 expression in the pathogenesis of CSCC.

Patients And Methods: The expression of APLP2 and a key modulator of cancer immune escape, MHC-I, were determined in CSCC tissue samples obtained from 141 patients using immunohistochemistry.

Proline-Hinged α-Helical Peptides Sensitize Gram-Positive Antibiotics, Expanding Their Physicochemical Properties to Be Used as Gram-Negative Antibiotics.

The outer membrane (OM) of Gram-negative bacteria is the most difficult obstacle for small-molecule antibiotics to reach their targets in the cytosol. The molecular features of Gram-negative antibiotics required for passing through the OM are that they should be positively charged rather than neutral, flat rather than globular, less flexible, or more increased amphiphilic moment. Because of these specific molecular characteristics, developing Gram-negative antibiotics is difficult.

The Relationship between MZ Generation Screen Golf Participants' Participation Motivation, Self-Esteem, and Psychological Happiness.

The purpose of this study is to analyze and clarify the relationship between the MZ generation's participation motivation in screen golf, self-esteem, and psychological happiness. To reach the goals of this study, 300 MZ generation screen golf participants were selected for this study. Accordingly, a questionnaire was distributed and 275 questionnaires were used for this study, excluding the answers that were omitted or unfaithful.

AamA-mediated epigenetic control of genome-wide gene expression and phenotypic traits in ATCC 17978.

Individual deletions of three genes encoding orphan DNA methyltransferases resulted in the occurrence of growth defect only in the (encoding Adenine Methylase A) mutant of strain ATCC 17978. Our single-molecule real-time sequencing-based methylome analysis revealed multiple AamA-mediated DNA methylation sites and proposed a potent census target motif (TTTRAATTYAAA). Loss of Dam led to modulation of genome-wide gene expression, and several Dam-target sites including the promoter region of the operon ( and ) were identified through our methylome and transcriptome analyses.

The ubiquitin-proteasome system links NADPH metabolism to ferroptosis.

Ferroptosis is the type of cell death arising from uncontrolled and excessive lipid peroxidation. NADPH is essential for ferroptosis regulation because it supplies reducing equivalents for antioxidant defense systems and contributes to the generation of reactive oxygen species. Moreover, NADPH level serves as a biomarker for predicting the sensitivity of cells to ferroptosis.

Marchf6 E3 ubiquitin ligase critically regulates endoplasmic reticulum stress, ferroptosis, and metabolic homeostasis in POMC neurons.

The metabolic prohormone pro-opiomelanocortin (POMC) is generally translocated into the endoplasmic reticulum (ER) for entry into the secretory pathway. Patients with mutations within the signal peptide (SP) of POMC or its adjoining segment develop metabolic disorders. However, the existence, metabolic fate, and functional outcomes of cytosol-retained POMC remain unclear.

Functional viromic screens uncover regulatory RNA elements.

The number of sequenced viral genomes has surged recently, presenting an opportunity to understand viral diversity and uncover unknown regulatory mechanisms. Here, we conducted a screening of 30,367 viral segments from 143 species representing 96 genera and 37 families. Using a library of viral segments in 3' UTR, we identified hundreds of elements impacting RNA abundance, translation, and nucleocytoplasmic distribution.

SIRT1 ubiquitination is regulated by opposing activities of APC/C-Cdh1 and AROS during stress-induced premature senescence.

SIRT1, a member of the mammalian sirtuin family, is a nicotinamide adenosine dinucleotide (NAD)-dependent deacetylase with key roles in aging-related diseases and cellular senescence. However, the mechanism by which SIRT1 protein homeostasis is controlled under senescent conditions remains elusive. Here, we revealed that SIRT1 protein is significantly downregulated due to ubiquitin-mediated proteasomal degradation during stress-induced premature senescence (SIPS) and that SIRT1 physically associates with anaphase-promoting complex/cyclosome (APC/C), a multisubunit E3 ubiquitin ligase.

Improvement of crAssphage detection/quantification method and its extensive application for food safety.

Water-borne diseases are usually caused by the fecal-oral transmission of human fecal pathogens. Traditionally, coliforms and enterococci are widely used as indicator bacteria, but they do not allow to differentiate between human and animal fecal contamination. Owing to its presence only in the human gut environment, crAssphage has been suggested as an alternative indicator of human fecal contamination to overcome the above challenges.

Systematic Review and Meta-Analysis on Burnout Owing to Perfectionism in Elite Athletes Based on the Multidimensional Perfectionism Scale (MPS) and Athlete Burnout Questionnaire (ABQ).

Previous studies have shown that burnout negatively affects athletes' mental health. To further explore this subject, we conducted a systematic review and meta-analysis by combining data from previous studies. This study followed the PRISMA guidelines for systematic and reliable research and completed data extraction using 10 databases and 8 keywords in December 2021.