Berbamine alleviates neuropathic pain via suppressing spinal TMEM34/SGK1/FOXO3 axis.

Background: The pathologic mechanism of neuropathic pain (NP) is still not fully understood, and efficient and safe therapeutic options are limited.

Purpose: Seeking new treatment options for neuropathic pain.

Study Design: Exploring new therapeutic target for NP and finding analgesic medications derived from traditional Chinese medicines against the target is urgent.

Development of cerebral microhemorrhages in a mouse model of hypertension.

Cerebral microhemorrhages (CMH) are the pathological substrate for MRI-demonstrable cerebral microbleeds, which are associated with cognitive impairment and stroke. Aging and hypertension are the main risk factors for CMH. In this study, we investigated the development of CMH in a mouse model of aging and hypertension.

Contrasting effects of temperature rise in different seasons on larger and smaller phytoplankton assemblages in a temperate coastal water, Laoshan Bay, northern Yellow Sea, China.

Anthropogenic influences and climate change are leading to more frequent and intense heatwaves, which are known to affect marine ecosystems. However, the effects of rising temperatures on in-situ phytoplankton size classes have not yet been adequately studied. In this study, two cell-sized phytoplankton assemblages (>20 μm, <20 μm) were cultured at a range of temperatures [i.

Advances in the biosynthesis of naturally occurring benzylisoquinoline alkaloids.

Benzylisoquinoline alkaloids (BIAs) are a prominent class of plant metabolites with significant pharmaceutical and industrial significance that have garnered substantial attention from researchers worldwide. BIAs exhibit several pharmacological activities and have been used extensively. Examples include analgesics such as morphine, tetrahydropalmatine, antimicrobials such as berberine, and antineoplastic agents including cepharanthine.

Integrated Network Pharmacology and Metabolomics to Investigate the Effects and Possible Mechanisms of Ginsenoside Rg Glycine Ester Derivative Against Hypoxia.

Previous studies have suggested that ginsenoside Rg glycine ester derivative (RG) exhibits therapeutic potential in mitigating hypoxia. This study aimed to elucidate the potential mechanism of RG in hypoxia injury through a combined approach of metabolomics and network pharmacology. Initially, a CoCl-induced cell hypoxia model was established, and the therapeutic impact of RG on biochemical indices was evaluated.

Potential Environmental Impacts and Management Strategies for Metal Release during Ocean Alkalinity Enhancement Using Olivine.

Ocean alkalinity enhancement (OAE) based on enhanced weathering of olivine (EWO) is a promising marine carbon dioxide removal (mCDR) technique. Previous research primarily focuses on the toxicological effects of potentially toxic metals (PTMs) released from olivine. In this Perspective, we explore the overlooked impacts of EWO on environmental media in two scenarios: olivine applied to beaches/shallow continental shelves and offshore dispersion by vessels.

To kill or to piggyback: Switching of viral lysis-lysogeny strategies depending on host dynamics.

Viruses wield significant influence over microbial communities and ecosystem function in marine environments. However, the selection of viral life strategies and their impacts on microbial communities remains enigmatic. In this study, we utilized a large-scale macrocosm, established using water samples from a marine coastal region, to enable community-level investigation.

The aqueous extract of Armadillidium vulgare Latreille alleviates neuropathic pain via inhibiting neuron-astrocyte crosstalk mediated by the IL-12-IFN-γ-IFNGR-CXCL10 pathway.

Ethnopharmacological Relevance: Armadillidium vulgare Latreille (AV), the dried body of pillbug, was originally described in Shennong's Classic of Materia Medica. As a common analgesic in animal-based traditional Chinese medicine, it is mainly used to relieve pain, promoting diuresis, relieving fatigue and so on. Our work demonstrated that AV could alleviate various types of acute and chronic pain including neuropathic pain (NP).

Synthesis, biological evaluation and mechanism study based on network pharmacology of amino acids esters of 20(S)-protopanaxadiol as novel anticancer agents.

As one of the metabolites of ginseng, 20(S)-protopanaxadiol (PPD) is a compound with dammarane-type tetracyclic triterpene, which performs a wide range of anticancer activities. In this study, PPD was used as a lead. A series of compounds were synthesized respectively with 11 amino acids through esterification and were evaluated for their cytotoxicity against several cancer cell lines.

Potential of CO sequestration by olivine addition in offshore waters: A ship-based deck incubation experiment.

Ocean alkalinity enhancement is considered as an effective atmospheric CO removal approach, but currently, little is known about the carbon sequestration potential of implementing olivine addition in offshore waters. We investigated the effect of olivine addition on the seawater carbonate system by carrying out a deck incubation experiment in the Northern Yellow Sea; the dissolution rate of olivine was calculated based on the increase in seawater alkalinity (TA), and the CO sequestration potential was evaluated. The results showed that the dissolution of olivine increased seawater TA and decreased partial pressure of CO, resulting in oceanic CO uptake from the atmosphere through sea-air exchange; it also increased seawater pH and mitigated ocean acidification to a certain extent.

New evidence supports the prophage origin of RcGTA.

Gene transfer agents (GTAs) are phage-like entities that package and transfer random host genome fragments between prokaryotes. RcGTA, produced by , is hypothesized to originate from a prophage ancestor. Most of the evidence supporting this hypothesis came from the finding of RcGTA-like genes in phages.

Globally distributed marine Gemmatimonadota have unique genomic potentials.

Background: Gemmatimonadota bacteria are widely distributed in nature, but their metabolic potential and ecological roles in marine environments are poorly understood.

Results: Here, we obtained 495 metagenome-assembled genomes (MAGs), and associated viruses, from coastal to deep-sea sediments around the world. We used this expanded genomic catalog to compare the protein composition and update the phylogeny of these bacteria.

Integrating bicarbonate-based microalgal production with alkaline sewage for ocean negative carbon emissions.

Using sewage (wastewater) for ocean alkalinity enhancement (OAE) has been considered as one promising ocean negative carbon emissions (ONCE) approach due to its high carbon sequestration efficiency and low environmental risk. To make this process more profitable and sustainable, this perspective proposes to integrate bicarbonate-based microalgal production and sewage alkalinity enhancement for ONCE. In this concept, the spent aqueous alkaline bicarbonate-based microalgal medium is cheap or even free for OAE, while the produced microalgae with high value-added compositions make this process more profitable.

Deep metagenomic sequencing unveils novel SAR202 lineages and their vertical adaptation in the ocean.

SAR202 bacteria in the Chloroflexota phylum are abundant and widely distributed in the ocean. Their genome coding capacities indicate their potential roles in degrading complex and recalcitrant organic compounds in the ocean. However, our understanding of their genomic diversity, vertical distribution, and depth-related metabolisms is still limited by the number of assembled SAR202 genomes.

Interfascial planes as surgical landmarks for laparoscopic upper retroperitoneal surgery: a cadaveric and retrospectively clinical study.

Background: Radiologists currently accept the concept of "interfascial plane (IFP)" to understand retroperitoneal anatomy, replacing Meyers' classic tricompartmental theory. Despite much research on retroperitoneal anatomy, its anatomical structure, embryonic origin and developmental process still require further exploration to guide the optimization of surgical process. This study aims to explore the anatomical basis of IFP related to laparoscopic upper retroperitoneal surgery (LURS) and to compare the clinical outcomes of trans-interfascial plane procedures for LURS (TIFP-LURS) with conventional LURS (Con-LURS).

The diversity and ecological significance of microbial traits potentially involved in B biosynthesis in the global ocean.

Cobalamin (B), an essential nutrient and growth cofactor for many living organisms on Earth, can be fully synthesized only by selected prokaryotes in nature. Therefore, microbial communities related to B biosynthesis could serve as an example subsystem to disentangle the underlying ecological mechanisms balancing the function and taxonomic make-up of complex functional assemblages. By anchoring microbial traits potentially involved in B biosynthesis, we depict the biogeographic patterns of B biosynthesis genes and the taxa harboring them in the global ocean, despite the limitations of detecting de novo B synthesizers via metagenomes alone.

Post-GWAS multiomic functional investigation of the TNIP1 locus in Alzheimer's disease highlights a potential role for GPX3.

Introduction: Recent genome-wide association studies (GWAS) have reported a genetic association with Alzheimer's disease (AD) at the TNIP1/GPX3 locus, but the mechanism is unclear.

Methods: We used cerebrospinal fluid (CSF) proteomics data to test (n = 137) and replicate (n = 446) the association of glutathione peroxidase 3 (GPX3) with CSF biomarkers (including amyloid and tau) and the GWAS-implicated variants (rs34294852 and rs871269).

Results: CSF GPX3 levels decreased with amyloid and tau positivity (analysis of variance P = 1.

Spartina alterniflora invasion benefits blue carbon sequestration in China.

Spartina alterniflora has rapidly and extensively encroached on China's coastline over the past decades. Among the coastal areas invaded by S. alterniflora, at most 93% are mudflats.

Sustainable carbon sequestration via olivine based ocean alkalinity enhancement in the east and South China Sea: Adhering to environmental norms for nickel and chromium.

Enhancing silicate weathering to increase oceanic alkalinity, thereby facilitating the absorption of atmospheric carbon dioxide (CO), is considered a highly promising technique for carbon sequestration. This study aims to evaluate the feasibility and potential of olivine-based ocean alkalinity enhancement (OAE) for the removal of atmospheric CO and its storage in seawater as bicarbonates in the East and South China Seas (ESCS). A particular focus is placed on the potential ecological impacts arising from the release of nickel (Ni) and chromium (Cr) during the olivine weathering process.

4'--MethylbavachalconeB Targeted 14-3-3ζ Blocking the Integrin β3 Early Outside-In Signal to Inhibit Platelet Aggregation and Thrombosis.

14-3-3ζ protein, the key target in the regulation and control of integrin β3 outside-in signaling, is an attractive new strategy to inhibit thrombosis without affecting hemostasis. In this study, 4'--methylbavachalconeB (4--MB) in Psoraleae Fructus was identified as a 14-3-3ζ ligand with antithrombosis activity by target fishing combined with ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) analysis. The competitive inhibition analysis showed that 4--MB targeted 14-3-3ζ and blocked the 14-3-3ζ/integrin β3 interaction with inhibition constant () values of 9.