Holographic multiplexing recording with an orthogonal polarized array.

This study combines tensor polarization holography theory and multichannel recording techniques and proposes a novel polarization encoding method, the orthogonal polarized array (OPA). This method can efficiently and independently reconstruct polarization holograms using accurate OPA waves in the reference-based multiplexing technique when the reference waves in the reading process have the same polarization state as those in the recording process. The novelty is that the multiplexing dimensions of the OPA can reach any number without limitations.

Anti-noise performance analysis in amplitude-modulated collinear holographic data storage using deep learning.

In an amplitude-modulated collinear holographic data storage system, optical system aberration and experimental noise due to the recording medium often result in a high bit error rate (BER) and low signal-to-noise ratio (SNR) in directly read detector data. This study proposes an anti-noise performance analysis using deep learning. End-to-end convolutional neural networks were employed to analyze noise resistance in encoded data pages captured by the detector.

Orthogonality of polarization superposition based on polarization holography.

We propose a polarization superposition orthogonal theory based on tensor polarization holography. Based on this theory, the holographic multiplexing capability can be improved measurably. The orthogonality of polarization waves is characterized by the null reconstruction in polarization holography, achieved through the superposition of multiple basic polarization reference waves.

Polarization variable line-space grating based on photoalignment liquid crystal.

The application of a liquid crystal (LC) in displays has driven the development of novel LC elements. In this Letter, polarization variable line-space (PVLS) gratings based on photoalignment are fabricated, and their variable-spacing properties are derived using the vector diffraction theory. Both transmissive and reflective PVLS gratings are fabricated to validate the correctness of the derivation.

Blue-shifted genetically encoded Ca indicator with enhanced two-photon absorption.

Significance: Genetically encoded calcium ion () indicators (GECIs) are powerful tools for monitoring intracellular concentration changes in living cells and model organisms. In particular, GECIs have found particular utility for monitoring the transient increase of concentration that is associated with the neuronal action potential. However, the palette of highly optimized GECIs for imaging of neuronal activity remains relatively limited.

Cross-species single-cell landscape of vertebrate pineal gland.

The pineal gland has evolved from a photoreceptive organ in fish to a neuroendocrine organ in mammals. This study integrated multiple daytime single-cell RNA-seq datasets from the pineal glands of zebrafish, rats, and monkeys, providing a detailed examination of the evolutionary transition at single-cell resolution. We identified key factors responsible for the anatomical and functional transformation of the pineal gland.

A blue-shifted genetically encoded Ca indicator with enhanced two-photon absorption.

Significance: Genetically encoded calcium ion (Ca) indicators (GECIs) are powerful tools for monitoring intracellular Ca concentration changes in living cells and model organisms. In particular, GECIs have found particular utility for monitoring the transient increase of Ca concentration that is associated with the neuronal action potential. However, the palette of highly optimized GECIs for imaging of neuronal activity remains relatively limited.

Wavefront coding image reconstruction via physical prior and frequency attention.

Wavefront coding (WFC) is an effective technique for extending the depth-of-field of imaging systems, including optical encoding and digital decoding. We applied physical prior information and frequency domain model to the wavefront decoding, proposing a reconstruction method by a generative model. Specifically, we rebuild the baseline inspired by the transformer and propose three modules, including the point spread function (PSF) attention layer, multi-feature fusion block, and frequency domain self-attention block.

Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator.

The ability to optically image cellular transmembrane voltages at millisecond-timescale resolutions can offer unprecedented insight into the function of living brains in behaving animals. Here, we present a point mutation that increases the sensitivity of Ace2 opsin-based voltage indicators. We use the mutation to develop Voltron2, an improved chemigeneic voltage indicator that has a 65% higher sensitivity to single APs and 3-fold higher sensitivity to subthreshold potentials than Voltron.

Effects of molecular weight fraction on antioxidation capacity of rice protein hydrolysates.

Rice protein was used as a starting material to provide rice protein hydrolysates (RPH) through enzyme-assisted extraction. RPH was further fractionated using ultrafiltration membrane (UF) and classified by molecular weight (MW; MW < 1 kDa, MW 1-10 kDa, and MW > 10 kDa). Peptides with MW < 1 kDa possessed superior antioxidant properties (p < 0.

Quantitative analysis on self-focusing properties of H-PDLC flexible curved radius gratings.

In general, the shape of traditional holographic grating is fixed and immutable, the period will not change after fabrication, this means that the modulation effect on the light field is unalterable. However, traditional grating cannot satisfy all requirements of current optical systems. In order to increase the versatility of holographic grating, a flexible curved radius grating (FCRG) which consists of holographic polymer-dispersed liquid crystal (H-PDLC) was proposed.

Disruption of the glucagon receptor increases glucagon expression beyond α-cell hyperplasia in zebrafish.

The glucagon receptor (GCGR) is a potential target for diabetes therapy. Several emerging GCGR antagonism-based therapies are under preclinical and clinical development. However, GCGR antagonism, as well as genetically engineered GCGR deficiency in animal models, are accompanied by α-cell hyperplasia and hyperglucagonemia, which may limit the application of GCGR antagonism.

Identification of MRAP protein family as broad-spectrum GPCR modulators.

Background: The melanocortin receptor accessory proteins (MRAP1 and MRAP2) are well-known endocrine regulators for the trafficking and signalling of all five melanocortin receptors (MC1R-MC5R). The observation of MRAP2 on regulating several non-melanocortin G protein-coupled receptors (GPCRs) has been sporadically reported, whereas other endogenous GPCR partners of the MRAP protein family are largely unknown.

Methods: Here, we performed single-cell transcriptome analysis and drew a fine GPCR blueprint and MRAPs-associated network of two major endocrine organs, the hypothalamus and adrenal gland at single-cell resolution.

Perovskite Solar Cells Employing a PbSO(PbO) Quantum Dot-Doped Spiro-OMeTAD Hole Transport Layer with an Efficiency over 22.

2,2',7,7'-Tetrakis(,-di--methoxyphenyl-amine)-9,9'-spirobifluorene (spiro-OMeTAD), the most widely used hole transport material in high-efficiency perovskite solar cells (PSCs), still has serious defects, such as moisture absorption and poor long-term conductivity, which seriously restrict further improvement of the power conversion efficiency (PCE) and stability of the cell. Herein, to overcome these problems, inorganic salt PbSO(PbO) quantum dots (QDs) are incorporated into spiro-OMeTAD as the hole transport layer (HTL) for the first time. The incorporated PbSO(PbO) QDs significantly hinder the agglomeration of lithium bis(trifluoromethanesulfonyl)-imide and improve the long-term conductivity through the oxidative interaction between PbSO(PbO) QDs and spiro-OMeTAD and hydrophobicity of the HTL.

Pharmacological modulation of the cAMP signaling of two isoforms of melanocortin-3 receptor by melanocortin receptor accessory proteins in the tetrapod Xenopus laevis.

As a member of the seven-transmembrane rhodopsin-like G protein-coupled receptor superfamily, the melanocortin-3 receptor (MC3R) is vital for the regulation of energy homeostasis and rhythms synchronizing in mammals, and its pharmacological effect could be directly influenced by the presence of melanocortin receptor accessory proteins (MRAPs), MRAP1 and MRAP2. The tetrapod amphibian Xenopus laevis (xl) retains higher duplicated genome than extant teleosts and serves as an ideal model system for embryonic development and physiological studies. However, the melanocortin system of the Xenopus laevis has not yet been thoroughly evaluated.

Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs.

PIWI-interacting small RNAs (piRNAs) protect the germline genome and are essential for fertility. piRNAs originate from transposable element (TE) RNAs, long non-coding RNAs, or 3´ untranslated regions (3´UTRs) of protein-coding messenger genes, with the last being the least characterized of the three piRNA classes. Here, we demonstrate that the precursors of 3´UTR piRNAs are full-length mRNAs and that post-termination 80S ribosomes guide piRNA production on 3´UTRs in mice and chickens.

Single-cell transcriptome analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma.

Diffuse large B cells in the cerebrospinal fluid (CSF-DLBCs) have offered great promise for the diagnostics and therapeutics of central nervous system lymphoma (CNSL) leptomeningeal involvement. To explore the phenotypic states of CSF-DLBCs, we analyzed the transcriptomes of more than one thousand CSF-DLBCs from six patients with CNSL diffuse large B-cell lymphoma (DLBCL) using Smart-seq2 single-cell RNA sequencing. CSF-DLBCs were defined based on abundant expression of B-cell markers, the active cell proliferation and energy metabolism properties, and immunoglobulin light chain restriction.

A rapid nucleic acid concentration measurement system with large field of view for a droplet digital PCR microfluidic chip.

Droplet digital polymerase chain reaction (ddPCR) is an effective technique, with unparalleled sensitivity, for the absolute quantification of target nucleic acids. However, current commercial ddPCR devices for detecting the gene chip are time consuming due to complex image stitching. To address this issue, we propose a universal concentration determination system and realize one-time gene chip imaging with high resolution.

Evaluating the Antioxidants, Whitening and Antiaging Properties of Rice Protein Hydrolysates.

Plant-derived protein hydrolysates have potential applications in nutrition. Rice protein hydrolysates (RPHs), an excellent source of proteins, have attracted attention for the development of cosmeceuticals. However, few studies have reported the potential application of RPH in analysis, and this study examined their antioxidant activities and the inhibitory activities of skin aging enzymes.

Pharmacological modulation of dual melanocortin-4 receptor signaling by melanocortin receptor accessory proteins in the Xenopus laevis.

Physiological modulation of melanocortin-4 receptor (MC4R) signaling by MRAP2 proteins plays an indispensable role in appetite control and energy homeostasis in the central nervous system. Great interspecies differences of the interaction and regulation of melanocortin receptors by MRAP protein family have been reported in several diploid vertebrates but never been investigated in the tetrapod amphibian Xenopus laevis. Here, we performed phylogenetic and synteny-based analyses to explore the evolutionary aspects of dual copies of X.

High-throughput and uniform large field-of-view multichannel fluorescence microscopy with super-thin dichroism for a dPCR gene chip.

With the rapid development of digital precision medicine, the digital polymerase chain reaction (dPCR) deoxyribonucleic acid (DNA) gene chip integrates more channels with smaller size and larger area, which leads to a higher technical requirement for commercial optical fluorescence microscopy. The multitime image splicing method is widely used for DNA detection. However, it consumes time and has visible seamless image results.

A general approach to engineer positive-going eFRET voltage indicators.

Imaging membrane voltage from genetically defined cells offers the unique ability to report spatial and temporal dynamics of electrical signaling at cellular and circuit levels. Here, we present a general approach to engineer electrochromic fluorescence resonance energy transfer (eFRET) genetically encoded voltage indicators (GEVIs) with positive-going fluorescence response to membrane depolarization through rational manipulation of the native proton transport pathway in microbial rhodopsins. We transform the state-of-the-art eFRET GEVI Voltron into Positron, with kinetics and sensitivity equivalent to Voltron but flipped fluorescence signal polarity.

Switchable pupil expansion propagation using orthogonal superposition varied-line-spacing H-PDLC gratings in a holographic waveguide system.

To solve the problem of small field of view in traditional holographic waveguides, this paper proposes a waveguide-coupling technique using orthogonal superposition varied-line-spacing (VLS) gratings. These gratings expand the x and y directions and orthogonally superimpose to achieve transmission in a waveguide and enlarge the pupil field of view. At the optical coupling input end and the coupling output end of a waveguide, one-dimensional VLS gratings are used to realize horizontal expansion of the image.

Bright and photostable chemigenetic indicators for extended in vivo voltage imaging.

Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility of existing GEVIs has been limited by the brightness and photostability of fluorescent proteins and rhodopsins. We engineered a GEVI, called Voltron, that uses bright and photostable synthetic dyes instead of protein-based fluorophores, thereby extending the number of neurons imaged simultaneously in vivo by a factor of 10 and enabling imaging for significantly longer durations relative to existing GEVIs.