Prep Biochem Biotechnol
February 2022
This study aimed to optimize the key parameters of extraction methods and to increase the recovery yields of intact xanthophylls (violaxanthin, zeaxanthin, astaxanthin) from microalgae (). An effective, simple, and fast extraction protocol is described. It consists of a grinding pretreatment followed by a microwave-assisted extraction, using ethanol 90% as an environmentally preferable extraction solvent.
View Article and Find Full Text PDFThe aeration is a key factor for Bacillus thuringiensis growth, sporulation and δ-endotoxins production. The objective of our work was to study the effect of aeration on the fermentation kinetics of Bacillus thuringiensis kurstaki (Btk), cultivated in a cereal milling byproduct (CMB) mono-component medium, in order to improve the δ-endotoxins productivity. Aeration conditions were systematically characterized by the volumetric mass transfer coefficient KLa.
View Article and Find Full Text PDFA simple new method, for separating Bacillus thuringiensis crystals from spores and cell debris, is described. The developed purification method uses hexane and low speed centrifugation and does not require any expensive material or reagents.
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