Shenqi Sanjie Granules induce Hmox1-mediated ferroptosis to inhibit colorectal cancer.

Background: Because adverse reactions or drug resistance are often found after current chemotherapies for metastatic colorectal cancer (mCRC), new treatments are still in demand. Shenqi Sanjie Granules (SSG), an antitumor compound preparation of traditional Chinese medicine, has been recognized for its ability in clinical practice of oncotherapy. Nevertheless, the precise effects of SSG in colorectal cancer (CRC) and underlying mechanisms through which SSG inhibits CRC remain uncertain.

The prognostic value of NLRP1/NLRP3 and its relationship with immune infiltration in human gastric cancer.

Background: Inflammasomes are related to tumorigenesis and immune-regulation. Here, we investigated the prognostic value of the NLR family pyrin domain containing (NLRP) 1/NLRP3 inflammasome and its potential mechanisms in immune-regulation in gastric cancer (GC).

Methods: We analyzed the differential expression of NLRP1/NLRP3 between tumor and normal tissues using the Oncomine and Tumor Immune Estimate Resource (TIMER) databases.

Pubertal exposure to acrylamide disrupts spermatogenesis by interfering with meiotic progression in male mice.

Teenagers are a major group likely to love junk foods, such as potato chips and bread items, which contain high levels of acrylamide (AA). The increasing evidence suggests that AA exposure may be associated with decreased reproductive capacity in humans and animals. However, the reproductive toxicity of AA in pubertal males has not been fully elucidated.

[Atrazine changes meiosis and reduces spermatogenesis in male mice].

Objective: To investigate the effects of exposure to atrazine on meiosis and spermatogenesis in adult male mice.

Methods: We divided 16 adult male Institute for Cancer Research (ICR) mice into a solvent control and an atrazine exposure group of an equal number and intraperitoneally injected with solvent dimethylsulfoxide (DMSO) and atrazine at 100 mg/kg/d, respectively. After 4 weeks of treatment, we obtained the body and testis weights of the mice, observed the changes in the testicular histomorphology, examined the cell apoptosis in the testis tissue, and determined the expressions of meiosis-related key genes in the spermatocytes by real-time fluorescence quantitative PCR.

Acrylamide impairs the developmental potential of germinal vesicle oocytes by inducing mitochondrial dysfunction and autophagy/apoptosis in mice.

Acrylamide (ACR), an important endogenous contaminant in carbohydrate-rich foods, has been involved in various negative effects on multiple organ networks, including the reproductive system. Previous studies have reported that ACR affects oocyte quality and fertility.  This study aimed to explore the toxic effects and regulatory mechanisms of ACR on mouse germinal vesicle (GV) oocytes.

Mitochondrial genome characterization of (Orthoptera: Gryllidae) and its phylogenetic implications.

is a cricket widely distributed throughout the world. In this study, we reported the first complete mitogenome sequence of Genus and inferred its phylogeny. The mitogenome of was 16,369 bp and consisted of a control region and a typical set of 37 genes.

Lactate-induced MRP1 expression contributes to metabolism-based etoposide resistance in non-small cell lung cancer cells.

Background: Metabolic reprogramming contributes significantly to tumor development and is tightly linked to drug resistance. The chemotherapeutic agent etoposide (VP-16) has been used clinically in the treatment of lung cancer but possess different sensitivity and efficacy towards SCLC and NSCLC. Here, we assessed the impact of etoposide on glycolytic metabolism in SCLC and NSCLC cell lines and investigated the role of metabolic rewiring in mediating etoposide resistance.

Molecular cloning and characterization of HSP60 gene in domestic pigeons (Columba livia) and differential expression patterns under temperature stress.

Heat shock protein 60 (HSP60) is a well-recognized multifunctional protein, playing a substantial role in protecting organisms from environmental stress. The domestic pigeon (Columba livia) is a promising model organism, with important economic and ecological value, and its health is susceptible to temperature stress. To explore the molecular characteristics, tissue expression profile, and response to temperature stress for HSP60 of Columba livia (ClHSP60), we firstly cloned and characterized the complete cDNA sequence and investigated its expression profile under optimal conditions and acute temperature stress.

[miR-449a/b negatively regulates E2F1 to suppress proliferation of gastric cancer cells].

Objective: To investigate the possible role of miR-449a/b in the occurrence of gastric cancer.

Methods: The expression of miR-449a/b and E2F1 mRNA in gastric cancer cells BGC-823 and gastric mucosal cells GES-1 were detected with qRT-PCR. miR-449a/b mimics or a negative control was transiently transfected into BGC-823 cells, and the changes in cell proliferation, apoptosis, and migration ability were assessed using CCK-8 assay, flow cytometry, and scratch wound healing assay, respectively.

A lactate-induced Snail/STAT3 pathway drives GPR81 expression in lung cancer cells.

Highly expressed G protein-coupled receptor 81 (GPR81), a receptor for lactate, is emerging as a critical regulator of tumor growth and metastasis. However, the mechanistic basis for its highly expression in cancer cells remains elusive. Here we report that tumor-derived lactate transcriptionally regulates GPR81 expression.

[Effects of cigarette smoke on the oxidative damage of mice oocytes and developmental potential].

Objective: To study the effects of cigarette smoke on the oxidative damage of mice oocytes and developmental potential.

Methods: A total of 96 SPF grade 6-week-old ICR female mice were randomly divided into control group, low-dose group, middle-dose group and high-dose group according to the principle of similar body weight. Twenty-four mice in each group were placed in a cigarette-lighting containers with the volume of 60, 32 and 23 L respectively.

[Effects of cigarette smoke on chromatin configuration, DNA methylation and expression of related genes in mice oocytes].

Objective: To study the effect of cigarette smoke on chromatin configuration, DNA methylation and expression of related genes in mice oocytes.

Methods: The clean grade ICR female mice were divided into 3 groups, control group, low dose group and high dose group. Animals were placed inside a perspex chamber( 18L) filled up with the smoke produced by 0, 1, 2 cigarettes, one hour a time, twice daily for 4weeks.

[Prediction and evolution of B cell epitopes of hemagglutinin in human-infecting H6N1 avian influenza virus].

Objective: To predict B cell epitopes of hemagglutinin (HA) of human-infecting H6N1 avian influenza virus and analyze their evolutionary characteristics.

Methods: The dataset was downloaded from GISAID and GenBank databases. And the linear and conformational B cell epitopes of HA were predicted separately by various bioinformatic software.

[Adaptive evolution of the hemagglutinin genes of the H6N1 avian influenza virus in Taiwan, China].

In Taiwan, the first human-infecting H6N1 avian influenza virus was isolated in 2013. To better understand the origin, evolutionary relationship and pathogenesis of the H6N1 virus, we studied the adaptive evolution and evolutionary dynamics of the hemagglutinin (HA) genes of the H6N1 virus in Taiwan. We felt that such studies woud contribute to the further study and control of the virus.