Fertility preservation in male adolescents with cancer (2011-2020): A retrospective study in China.

Background: According to the studies, more than 80% of pediatric patients with cancer can achieve a survival rate greater than 5 years; however, long-term chemotherapy and/or radiation therapy may seriously affect their reproductive ability. Fertility preservation in adolescents with cancer in China was initiated late, and related research is lacking. Analyze data to understand the current situation and implement measures to improve current practices.

Fertility preservation in males with cancer of trends, region development, and efficacy in mainland China from 16 regions Chinese sperm banks.

Purpose: Male cancer survivors experience confusion about fertility following cancer treatment. The aims of this study were to evaluate survivors' semen quality in different tumor type groups in China and to analyze the current situation and challenges of male cancer patients with sperm cryopreservation.

Methods: This was a multicenter retrospective study of male patients with cancer who underwent sperm cryopreservation in 16 regions of the national sperm banks over an 11-year period from 2010 to 2020.

A novel variant in CFAP69 causes asthenoteratozoospermia with treatable ART outcomes and a literature review.

Purpose: Multiple morphological abnormalities of the sperm flagella (MMAF) are a severe form of sperm defect causing male infertility. Previous studies identified the variants in the CFAP69 gene as a MMAF-associated factor, but few cases have been reported. This study was performed to identify additional variants in CFAP69 and describe the semen characteristics and outcomes of assisted reproductive technology (ART) in CFAP69-affected couples.

Circulating microRNAs in seminal plasma as predictors of sperm retrieval in microdissection testicular sperm extraction.

Background: Because of focal spermatogenesis in some nonobstructive azoospermia (NOA) patients, testicular spermatozoa can be retrieved by microdissection testicular sperm extraction (micro-TESE) for intracytoplasmic sperm injection (ICSI) to achieve successful fertilization. Currently, testicular biopsy is widely performed for the prognosis of micro-TESE; however, it might miss foci with active spermatogenesis because of the 'blind manner' of puncture, highlighting the needs for biomarkers that could indicate actual spermatogenesis conditions in the testis. Thus, we screened microRNAs in the seminal plasma for potential biomarkers to provide a non-invasive and reliable preoperative assessment for micro-TESE.

[Bacterial culture of donor semen before and after cryopreservation: A preliminary study].

Objective: To investigate the factors causing bacterial contamination of donor semen during cryopreservation.

Methods: Based on the WHO Laboratory Manual for the Examination and Processing of Human Semen (5th ed), we diluted donor semen samples with sperm cryopreservation medium in a 1∶1 (v/v) ratio. Before the experiment, we cultured bacteria in the sperm cup, sperm cryopreservation medium and Columbia blood a-gar medium to ensure sterile growth.

[Characterization of the men undergoing autologous sperm preservation in Chongqing Human Sperm Bank].

Objective: To analyze the characteristics of the males undergoing autologous sperm preservation (ASP), and provide some reference for human sperm banks to offer targeted service for those undergoing ASP.

Methods: We statistically analyzed the demographic features, reasons for ASP, and other relevant factors of the men applying for ASP in Chongqing from January 2016 to December 2019.

Results: Within 4 years, a total of 76 males applied for ASP, of whom about 63.

[Bacteriological analysis of semen samples from sperm donors].

Objective: To explore the distribution and characteristics of semen bacteria in sperm donors.

Methods: For lack of the reference value for semen bacterial culture and colony count, we referred to the existing criteria for the diagnosis of male urinary tract infection (urine bacterial colonies >105 cfu/ml for infection, <104 cfu/ml for possible bacterial contamination, and 104－105 cfu/ml for suspected infection) and set the threshold value for unqualified semen culture at bacterial colonies ≥104cfu/ml. We performed bacterial culture of the semen samples from 3 160 sperm donors in the Chongqing Human Sperm Bank from April 2015 to November 2019, counted the bacterial colonies using the British Synbiosis automatic colony counter, and identified the bacterial species in the samples with bacterial colonies ≥104cfu/ml with the automatic microbial identification instrument.

[Efficiency of sperm donation: An analysis of 440 qualified sperm donors in Chongqing Human Sperm Bank].

Objective: To analyze the efficiency of sperm donation by qualified donors and provide some experience for improving the success rate of sperm donation in human sperm banks.

Methods: This study included 440 qualified sperm donors in Chongqing Human Sperm Bank from April 2015 to June 2019. We analyzed the general information about the donors, the causes of failed sperm donation and the results of semen bacterial culture.

[Bacterial culture of donor semen: Analysis of results].

Objective: To investigate bacterial infection and the distribution of different bacterial species in the donor semen and the influence of different bacterial counts on semen quality.

Methods: Bacterial colonies in the semen samples from 1 126 donors were counted with the Synbiosis Protocol 3 Automatic Colony Counter and the bacterial species with a colony count ≥10⁴ cfu/ml identified with the VITEK2 Compact Automatic Biochemical Analyzer. The Makler Sperm Counting Board was used to examine the semen quality of the semen samples with a colony count = 0 cfu/ml (n = 22, group A), those with a colony count <10⁴ cfu/ml (n = 22, group B) and those with a colony count ≥10⁴ cfu/ml (n = 22, group C).

[An analysis of donor semen quality in Chongqing Human Sperm Bank].

Objective: To evaluate the quality of the donor semen in Chongqing Human Sperm Bank and the influence of age on semen parameters.

Methods: We collected semen samples from 899 donors in Chongqing Human Sperm Bank and divided them into five groups according to the age of the semen donors: 22－25, 26－30, 31－35, 36－40, and >40 years old. Using the Makler Counting Chamber, we measured the semen volume, percentage of progressively motile sperm (PMS), total motile sperm, sperm concentration, total sperm count per ejaculate, and percentage of morphologically normal sperm (MNS).

Involvement of hypoxia-inducible factor-1α in the oxidative stress induced by advanced glycation end products in murine Leydig cells.

Hyperglycemia increases the formation of advanced glycation end products (AGEs), triggers oxidative impairments and influences inducible factor (HIF)-1α protein levels and transactivation function. Compromised HIF-1α in testis leads to male infertility. The aim of the study was to investigate the role of HIF-1α in oxidative stress induced by AGEs in murine Leydig TM3 cells.

The dynamics of polycomb group proteins in early embryonic nervous system in mouse and human.

Polycomb group (PcG) proteins are transcription regulatory proteins that control the expression of a variety of genes and the antero-posterior neural patterning from early embryogenesis. Although expression of PcG genes in the nervous system has been noticed, but the expression pattern of PcG proteins in early embryonic nervous system is still unclear. In this study, we analyzed the expression pattern of PRC1 complex members (BMI-1 and RING1B) and PRC2 complex members (EED, SUZ12 and EZH2) in early embryonic nervous system in mouse and human by Western blot and Immunohistochemistry.

[Birth defects among children aged 0-4 in Chongqing in 2010].

Objective: To learn the prevalence of birth defects in Chongqing.

Methods: A total of 6579 children aged 0 - 4 were chosen by multistage cluster sampling method in central economic districts of Chongqing. A total of 32 kinds of birth defects were selected.

[An analysis on chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization].

Objective: To analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization.

Methods: The experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization (FISH) and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa.