Publications by authors named "Jieyu Cui"
Article Synopsis
- Scientists are trying to improve gene editing in cells by using new techniques that help fix DNA more effectively.
- They developed smaller proteins (SlugCas9 and AsCas12a) that make it easier to use these techniques compared to bigger proteins that were hard to handle.
- Tests showed that these new methods worked better and achieved higher gene editing efficiency, making it easier for researchers to use gene editing in their studies.
[A novel CRISPR/Cas9-hLacI donor adapting system for dsDNA-templated gene editing].
Sheng Wu Gong Cheng Xue Bao
October 2023
During the gene editing process mediated by CRISPR/Cas9, precise genome editing and gene knock-in can be achieved by the homologous recombination of double-stranded DNA (dsDNA) donor template. However, the low-efficiency of homologous recombination in eukaryotic cells hampers the development and application of this gene editing strategy. Here, we developed a novel CRISPR/Cas9-hLacI donor adapting system (DAS) to enhance the dsDNA-templated gene editing, taking the advantage of the specific binding of the LacI repressor protein and the LacO operator sequence derived for the lactose operon.
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- Researchers developed a new Donor Adapting System (DAS) to enhance the efficiency of homology-directed repair (HDR) in CRISPR gene editing by using a Gal4 DNA binding domain in conjunction with the Cas9 protein.
- The study showed that using a specific length of homologous arms (100-60 bp) in the donor DNA could improve HDR editing efficiency by 2-4 times in a cell line experiment.
- Further optimizations indicated that the choice of fusion components and aptamer designs significantly impact Cas9's performance and HDR success, leading to effective editing at specific genetic sites, which could aid in animal breeding and molecular design.
Effects of Microwave-Assisted Liquid Hot Water Pretreatment on Chemical Composition and Structure of Moso Bamboo.
Front Bioeng Biotechnol
February 2022
The effects of microwave assisted liquid hot water (MA-LHW) pretreatment on the chemical composition of Moso bamboo were investigated, and the fiber structure of pretreated residues were studied. The results showed that MA-LHW pretreatment had high selectivity for the degradation of hemicellulose in Moso bamboo, and the extracted hemicellulose could be used to prepare xylooligosaccharide through enzyme depolymerization. The degradation rates of cellulose and lignin after MA-LHW pretreatment were only 14.
View Article and Find Full Text PDFAlginic acid (AA) is a kind of polysaccharide extracted from brown seaweeds and has been widely used in food industry. Certain positive effects of AA, such as anti-inflammation and anti-allergy, have been reported. Nevertheless, as a potential chemical contaminant of the environment, its impact on female reproductive system remains to be investigated.
View Article and Find Full Text PDFCucumber fusarium wilt is a soil-borne disease which causes serious production decrease in cucumber cultivation world widely. Extensive using of chemical pesticides has caused serious environmental pollution and economic losses, therefore, it is particularly urgent to develop efficient, safe and pollution-free biopesticide. In this study, a mutant strain of cultivated in moso bamboo medium was proved to be an efficient bio-inhibitor of the disease.
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