The value of contrast-enhanced ultrasound in predicting postoperative recurrence of hepatocellular carcinoma: A protocol for systematic review and meta-analysis.

Background: As one of the key factors, postoperative recurrence of hepatocellular carcinoma (HCC) influences the therapeutic effects and survival period of patients. Therefore, the early diagnosis of postoperative recurrence of HCC plays an important role in improving the therapeutic effects and prognosis. Contrast-enhanced ultrasound (CEUS) plays an important role in the early diagnosis of postoperative recurrence of HCC.

Impeded Nedd4-1-mediated Ras degradation underlies Ras-driven tumorigenesis.

RAS genes are among the most frequently mutated proto-oncogenes in cancer. However, how Ras stability is regulated remains largely unknown. Here, we report a regulatory loop involving the E3 ligase Nedd4-1, Ras, and PTEN.

[Clinical treatment of 29 cases of acne of "yin-deficiency-inner heat type" by suspended moxibustion].

Objective: To observe the clinical effect of moxibustion therapy for 29 cases of yin-deficiency type acne.

Methods: In this clinical trial, 56 cases of acne patients with yin-deficiency-inner heat type were enrolled. These patients were randomly divided into moxibustion group (treated by suspended moxibustion, n = 29) and control group (treated by acupuncture, n = 27).

Quantitative analysis of aberrant fatty acid composition of zebrafish hepatic lipids induced by organochlorine pesticide using stable isotope-coded transmethylation and gas chromatography-mass spectrometry.

Organochlorine pesticides have been extensively used worldwide for agricultural purposes. Due to their resistance to metabolism, a major public health concern has been raised. Aberrant hepatic lipid composition has been a hallmark of many liver diseases associated with exposure to various toxins and chemicals.

Measurement of laser activated electron tunneling from semiconductor zinc oxide to adsorbed organic molecules by a matrix assisted laser desorption ionization mass spectrometer.

Measurement of light induced heterogeneous electron transfer is important for understanding of fundamental processes involved in chemistry, physics and biology, which is still challenging by current techniques. Laser activated electron tunneling (LAET) from semiconductor metal oxides was observed and characterized by a MALDI (matrix assisted laser desorption ionization) mass spectrometer in this work. Nanoparticles of ZnO were placed on a MALDI sample plate.

Desalting of phosphopeptides by tandem polypyrrole-c18 reverse phase micropipette tip (TMTip(PPY-C18)) based on hybrid electrostatic, Π-Π stacking and hydrophobic interactions for mass spectrometric analysis.

Desalting and concentration of peptides using reverse phase (RP) C18 chromatographic material based on hydrophobic interaction is a routine approach used in mass spectrometry (MS)-based proteomics. However, MS detection of small hydrophilic peptides, in particular, phosphopeptides that bear multiple negative charges, is challenging due to the insufficient binding to C18 stationary phase. We described here the development of a new desalting method that takes the unique properties of polypyrrole (PPY).

Quantification of Interactions between Serum Albumin and Endogenous Free Fatty Acids or Exogenous Chemicals by Stable Isotope-Coded Mass Spectrometry.

As primary endogenous ligands of serum albumin, free fatty acids exert versatile effects on the albumin conformation through cooperative or competitive interactions with exogenous chemicals. Based on equilibrium partition between n-hexane and aqueous phases, we have established three indexes, defined as R A, R V, and R T, for quantitative assessment of the intrinsic binding affinity, the affinitive variation induced by exogenous chemicals, and the topological dependence of albumin affinity, respectively. When albumin molecules in the aqueous phase are in native or denatured forms, or disturbed by exogenous chemicals, corresponding changes of free fatty acids in the n-hexane phase can be quantified by an iFFAM (isotope-coded free fatty acid methylation) approach.

Typing of unknown microorganisms based on quantitative analysis of fatty acids by mass spectrometry and hierarchical clustering.

Rapid identification of unknown microorganisms of clinical and agricultural importance is not only critical for accurate diagnosis of infections but also essential for appropriate and prompt treatment. We describe here a rapid method for microorganisms typing based on quantitative analysis of fatty acids by iFAT approach (Isotope-coded Fatty Acid Transmethylation). In this work, lyophilized cell lysates were directly mixed with 0.

[Effect of recombinant microplasmin on acute cerebral infarction in rats].

The effect of recombinant microplasmin (micro-plasmin) on acute cerebral infarction was evaluated in rats, and compared with recombinant tissue plasminogen activator (rt-PA). After the model of middle cerebral artery occlusion (MCAO) was established by autologous blood clots, different doses of micro-plasmin (2.5, 5, and 10 mg x kg(-1)) were administered into the thrombus intra-arterial.

The thrombolytic effect of miniplasmin in a canine model of femoral artery thrombosis.

Background And Purpose: Miniplasmin was a des-kringle variant of plasminogen with potential pharmacological application. We investigated the thrombolytic effect of miniplasmin in a canine model of femoral artery thrombosis.

Methods: In anesthetized dogs, a stable occlusive thrombus was formed by mechanical and electrolytic injury of the vessel wall, that the animals were later injected with miniplasmin (0.

[Clinical observation on the regularity of acupuncture-induced body-reduction in excess-heat-type obesity patients ].

Objective: To investigate the regularity of acupuncture of the Stomach Meridian for excess-heat-type obesity.

Methods: Fifty-one patients with gastrointestine excess-heat syndrome were randomly divided into treatment group (n = 25) and control group (n = 26) according to the random number table method. Patients of treatment group were treated mainly with acupuncture of acupoints of the Stomach Meridian as bilateral Liangmen (ST 21), Huaroumen (ST 24), Tianshu (ST 25), Wailing (ST 26), etc.