Aims: To establish a FF1-ATP synthase molecular motor biosensor to accurately identify colon cancer miRNAs.
Main Methods: The FF1-ATP synthase molecular motor is extracted by fragmentation-centrifugation and connected to the colon cancer-specific miR-17 capture probe in the manner of the ε subunit-biotin-streptavidin-biotin system. Signal probes are designed for dual-signal characterization to increase detection accuracy.