Single-cell meta-analysis of inflammatory bowel disease with scIBD.

Understanding the heterogeneous intestinal microenvironment is critical to uncover the pathogenesis of inflammatory bowel disease (IBD). Recent advances in single-cell RNA sequencing (scRNA-seq) have identified certain cell types and genes that could contribute to IBD; however, a comprehensively integrated analysis of these scRNA-seq datasets is not yet available. Here we introduce scIBD, a platform for single-cell meta-analysis of IBD with interactive and visualization features, which combines highly curated single-cell datasets in a uniform workflow, enabling identifying rare or less-characterized cell types in IBD and dissecting the commonalities, as well as the differences between ulcerative colitis and Crohn's disease.

Structure and activity alteration in adult highland residents' cerebrum: Voxel-based morphometry and amplitude of low-frequency fluctuation study.

Introduction: People living in highland areas may have factors that allow them to adapt to chronic hypoxia, but these physiological mechanisms remain unclear. This study aimed to investigate the brain mechanism in a cohort of adult residents of Tibet, a well-known plateau section in China, by observing differences in brain structure and function in non-plateau populations.

Methods: The study included 27 Tibetan and 27 non-plateau region residents who were matched in age, sex, and education.

A Bioinformatic Framework for Dissecting the Dynamics of T Cells from Single-Cell Transcriptome.

The quantitative tracking of the dynamics of T cells is challenging in human immunology. Although bulk sequencing of T cell receptor (TCR) α- and β-chains has been widely used for determining the clonality of T cells, such methods are limited in unveiling the phenotypic differences of T cells with the same clonotypes. Here, we describe a bioinformatics framework, STARTRAC, that integrates the single-cell transcriptome and TCR sequences as lineage-specific markers to quantitatively assess the dynamics of T cells, including their clonal expansion, tissue migration, and developmental transition properties.

Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients.

Objective: A comprehensive immune landscape for HBV infection is pivotal to achieve HBV cure.

Design: We performed single-cell RNA sequencing of 2 43 000 cells from 46 paired liver and blood samples of 23 individuals, including six immune tolerant, 5 immune active (IA), 3 acute recovery (AR), 3 chronic resolved and 6 HBV-free healthy controls (HCs). Flow cytometry and histological assays were applied in a second HBV cohort for validation.

COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas.

A dysfunctional immune response in coronavirus disease 2019 (COVID-19) patients is a recurrent theme impacting symptoms and mortality, yet a detailed understanding of pertinent immune cells is not complete. We applied single-cell RNA sequencing to 284 samples from 196 COVID-19 patients and controls and created a comprehensive immune landscape with 1.46 million cells.

[Cognition function and its influencing factors among people aged 55 and above in 4 provinces of China from 2018 to 2019].

Objective: To analyze the current situation of cognition function of people aged 55 and above in 4 provinces of China, and to explore its influencing factors of demographic characteristics.

Methods: Using the baseline data of the "Community-based Cohort Study on Nervous System Diseases", middle-aged and older populations aged ≥55 years with completed data on demographic and economic factors and the cognitive function scale were selected as study subjects. A total of 5103 subjects were included in the study(male 2294, female 2809, 55-64 years old 1875, 65-74 years old 2197, 75-94 years old 1031).

Landscape of transcript isoforms in single T cells infiltrating in non-small-cell lung cancer.

Single-cell RNA sequencing (scRNA-seq) has enabled high-resolution characterization of molecular signatures of tumor-infiltrating lymphocytes. However, analyses at the transcript isoform level are rarely reported. As alternative splicing is critical to T-cell differentiation and activation, here, we proposed a computational method named IDEA (Isoform Detection, Enrichment, and functional Annotation) to comprehensively detect and annotate differentially used isoforms across cell subtypes.

[Dandelion clock-like sign on CT for diagnose of COVID-19].

Objective: To explore the value of the "dandelion clock-like" sign on chest CT for diagnosis of SARS-CoV-2-associated pneumonia.

Methods: This retrospective analysis was conducted based on the data of 119 cases from the Department of Fever and the Department of Infection undergoing chest high-resolution CT examinations in Sanshui District People's Hospital between January, 24 and February 10, 2020. The cases with no abnormality on chest CT were excluded.

Visible-light-mediated deuteration of silanes with deuterium oxide.

Isotopically labeled compounds are highly desirable as they can serve as both mechanistic probes in chemistry and diagnostic tools in medicinal research. Herein, we report an unprecedented visible-light-mediated metal-free deuteration of silanes using DO as an inexpensive, readily available, and easy to handle deuterium source. A broad range of aryl- and alkyl-substituted silanes were deuterated with high deuterium incorporations and yields.

Tetraphenylethene (TPE) modified polyhedral oligomeric silsesquioxanes (POSS): unadulterated monomer emission, aggregation-induced emission and nanostructural self-assembly modulated by the flexible spacer between POSS and TPE.

Mono-TPE modified POSS molecules, in which the flexible spacers between TPE and POSS moieties control their self-assembly and aggregation, exhibit a unique unadulterated monomer emission in organic solvents as well as an AIE emission in THF/water.