A Trojan horse biomimetic delivery system using mesenchymal stem cells for HIF-1α siRNA-loaded nanoparticles on retinal pigment epithelial cells under hypoxia environment.

Aim: To demonstrate the feasibility of mesenchymal stem cell (MSC)-mediated nano drug delivery, which was characterized by the "Trojan horse"-like transport of hypoxia-inducible factor-1α small interfering RNA (HIF-1α siRNA) between MSCs and retinal pigment epithelial cells (RPE) under hypoxia environment.

Methods: Plasmid and lentivirus targeting the human HIF-1α gene were designed and constructed. HIF-1α siRNA was encapsulated into poly(lactic-co-glycolic acid) nanoparticles (PLGA-NPs) through the water-in-oil-in-water (w/o/w) multiple emulsion technique.

Sesquiterpenes From Stems and Their Anti-Photoaging Effects by Down-Regulating Matrix Metalloproteinase-1 Expression Anti-Inflammation.

In the process of continuing to investigate ultraviolet b (UVB) irradiation protective constituents from stems, nine new sesquiterpenes, named as eurylosesquiterpenosides A-D (), eurylosesquiterpenols E-I (), and ten known ones () were gained. Their structures were established by analysis of their NMR spectroscopic data, and electronic circular dichroism calculations were applied to define their absolute configurations. In addition, UVB induced HaCaT cells were used to study their anti-photoaging activities and mechanism.

The phenolic acids from Oplopanax elatus Nakai stems and their potential photo-damage prevention activity.

25 phenolic acids, including four new isolates, eurylophenosides A-D (1-4) and 21 known ones (5-25) were isolated and identified from the stems of Oplopanax elatus Nakai. Among the known compounds 5-9, 11-13, 16, 18-25 were isolated from the genus for the first time; 17 was first obtained from the plant; and the NMR data of 22 was reported here first. Meanwhile, the UVB-induced photodamage model of HaCaT cells was used to study the prevent-photodamage abilities of compounds 1-2, 4-8, 11-13 and 15-25 with a nontoxic concentration at 50 μM.

Correction to: The structure-activity relationship review of the main bioactive constituents of Morus genus plants.

The article The structure-activity relationship review of the main bioactive constituents of Morus genus plants, written by Jiejing Yan, Jingya Ruan, Peijian Huang, Fan Sun, Dandan Zheng, Yi Zhang and Tao Wang was originally published Online First without Open Access.

Anti-inflammatory Limonoids From .

The root barks of perennial herb () were reported to be rich in anti-inflammation activity constituents, limonoids. Then, the investigation of anti-inflammation therapeutic limonoids from this plant was developed in the present study. Through the combination of various chromatographies isolation, six new limonoids, named dictamlimonol A (), dictamlimonoside B (), and dictamlimonols C-F (-), along with seven known ones (-), were obtained.

NMR and MS data for novel bioactive constituents from L. Gaertn.

The data presented in this article are associated with the research article entitled "" [1]. The aim of this data was to provide the 1D, 2D NMR and MS spectrum of novel bioactive compounds from .

New Dammarane-Type Triterpenoid Saponins from Leaves and Their Nitric Oxide Inhibitory Activities.

Inflammation is a very common and important pathological process that can cause many diseases. The discovery of anti-inflammatory drugs and the treatment of inflammation are particularly essential. Dammarane-type triterpenoid saponins (PNS) were demonstrated to show anti-inflammatory effects in the leaves of .

The structure-activity relationship review of the main bioactive constituents of Morus genus plants.

Morus genus plants are mainly distributed in the temperate to tropical areas over the world and include 17 species and two subspecies. Due to their excellent pharmacological activity, security in food additives and high value in the national economy, Morus genus plants have drawn more and more attention in recent years. In the light of the references published over the last few decades, flavonoids, benzofurans, stilbenes, and Diels-Alder adducts have been reported to be the main bioactive constituents of Morus genus plants.

Bioactive constituents study of Pugionium cornutum L. Gaertn on intestinal motility.

Thirty-one compounds were obtained and identified from the dried roots of Mongolian medicinal and edible plant, Pugionium cornutum L. Gaertn. Eight of them were new ones, and named as pugcornols A (1), B (2), C (3), and D (4), pugcornosides A (5), B (6), C (7), and D (8), respectively.

Comprehensive Chemical Profiling in the Ethanol Extract of Aerial Parts by Liquid Chromatography/Mass Spectrometry Analysis of Its Silica Gel Column Chromatography Fractions.

Less. is a medicine and food dual-use plant, which belongs to the Pluchea genus, Asteraceae family. Its main constituents are quinic acids, flavonoids, thiophenes, phenolic acids, as well as sesquiterpenes.

Relationship between Structural Characteristics and Plant Sources along with Pharmacology Research of Quassinoids.

Quassinoids, one kind of triterpenoids with multiple bioactivities such as anti-cancer, anti-malarial, anti-oxidative, anti-microbial, anti-diabetic, anti-viral, and anti-inflammatory effects, have drawn much attention in recent years. Between 2004 and 2018, the structural characteristics and plant sources of 190 quassinoids were reported. Herein, the structure-activity relationships (SARs) of quassinoids along with the anti-cancer mechanisms of four representative quassinoids, eurycomanone, bruceine D, dehydrobruceine B, and brusatol are discussed.

Anti-inflammatory constituents from Cortex Dictamni.

Eight new compounds named as dictamalkosides A (1), B (2), C (3), dictamphenosides A (4), B (5), C (6), D (7) and E (8), as well as 23 known ones were obtained from the 70% EtOH extract of Cortex Dictamni. Their structures were ascertained based on the spectroscopic evidences. Among the known compounds, 14, 17-23, 25-28, and 31 were isolated from Dictamnus genus for the first time; 16 and 24 were firstly isolated from this plant.

Separation and Bioactive Assay of 25/-Spirostanol Saponin Diastereomers from Roezl (Mojave) Stems.

In order to find a simple, generic, efficient separation method for 25/-spirostanol saponin diastereomers, the liquid chromatographic retention behaviors of C carbonylation and C unsubstituted 25/-spirostanol saponin diastereomers on different stationary phases (C₈, C, C columns) and different mobile phases (MeOH-1% CH₃COOH and CH₃CN-1% CH₃COOH) were investigated. A C column was firstly found to offer the highest efficiency for the separation of this kind of diastereomers than C₈ and C columns. Meanwhile, the analysis results indicated that both CH₃CN-1% CH₃COOH and MeOH-1% CH₃COOH eluate systems were selective for C unsubstituted 25/-spirostanol saponin diastereomers, while MeOH-1% CH₃COOH possessed better selectivity for C carbonylation ones.

Bioactive Constituents from the Aerial Parts of Less.

Four new thiophenes, (3'')-pluthiophenol (), (3'')-pluthiophenol-4''-acetate (), 3''-ethoxy-(3'')-pluthiophenol (), 3''-ethoxy-(3'')-pluthiophenol-4''-acetate (), together with twenty-five known compounds were obtained from the 70% ethanol-water extract of the aerial parts of Less. Their structures were elucidated by spectroscopic methods. Among the known isolates, compounds , , , , , , , , ⁻ were isolated from Asteraceae family firstly, while compounds , , , , , , , , were isolated from genus for the first time.

[Hypoxia and hyperglycemia promote the proliferation and migration of human marrow mesenchymal stem cells in vitro].

Objective: To investigate the cell proliferation, apoptosis and migration of human marrow mesenchymal stem cells (hMSCs) under hypoxia and hyperglycemia.

Methods: The hMSCs from healthy adults were isolated and harvested by density gradient centrifugation followed by adherent cultures. The cells was divided into four groups: control group (210 mL/L O2 with 5.