[Molecular characteristics of HIV-1 CRF01_AE strains in Zhejiang province in 2009].

Objective: To analyze the molecular epidemiological characteristics of HIV-1 subtype CRF01_AE strains being prevailed among HIV/AIDS in Zhejiang province in 2009.

Methods: A total of 303 subjects were identified by stratified random sampling among HIV infected individuals in Zhejiang province in 2009. Gag fragments of the HIV-1 strains were amplified by nested polymerase chain reaction from the DNA extracted from whole blood of HIV-1 infected individuals.

[Survey of HIV drug resistance threshold in Zhejiang province from 2009 to 2011].

Objective: To survey the prevalence of drug resistant HIV in Zhejiang province in 2009-2011.

Methods: WHO truncated sequential sampling technique was adopted annually by using 63, 62 and 57 samples of newly diagnosed as HIV positive and aged 16-25 years in Hangzhou, Ningbo and Wenzhou from 2009 to 2011, respectively. RNA was prepared and HIV pol region was amplified by RT-PCR and nested PCR.

[Relationship between strain subtypes and transmission of HIV infection within marriage-based immigrant women in rural area of Zhejiang province].

Objective: To analyze the relationship between strain subtypes and transmission of HIV infection on marriage-based immigrant women, their spouses and children in rural area of Zhejiang province.

Methods: Marriage-based immigrant women with HIV infection, their HIV infected spouses and children in rural area in Zhejiang province, were selected as study objects. Analysis on genetic sequence and epidemiologic information was carried out.

[Analysis of epidemiological characteristics of HIV infections among immigrant marriage women in rural areas in Zhejiang province].

Objective: To analyze the epidemiological characteristics of HIV infected immigrant marriage women in rural areas in Zhejiang province.

Methods: A retrospective investigation was conducted among 118 HIV infected immigrant marriage women, who were detected in active or passive surveillance in rural areas in Zhejiang province. The investigation information included the basic demographic characteristics, risky behavior, the status of HIV infection among their spouses and children.

[Subtype and transmission of HIV strain in both HIV infected spouses in Zhejiang province].

Objective: To analyse the subtype and transmission of HIV strain in both HIV infected spouses.

Methods: Reported both HIV infected spouses were selected as objects. Analysis on genetic sequence and high risk behaviors was carried out.

[Genotypic drug-resistance of HIV-1 CRF01_AE in Zhejiang province, 2004-2007].

Objective: To study the genotypic drug-resistant mutation among treat-naïve or treated patients infected with HIV-1 CRF01_AE in Zhejiang province during 2004-2007.

Methods: HIV-1 pol amplicons (PR + RT) from 13 treated and 43 treat-naïve patients were obtained by reverse transcription-polymerase chain reaction (RT-PCR). The sequences were analyzed for genotypic antiretroviral resistance through online tools (http://hivdb.

[Molecular characteristics of HIV-1 CRF01 _ AE strains in Zhejiang province].

Objective: Using molecular epidemiology method to characterize human immunodeficiency virus type 1 (HIV-1) subtype CRF01 _ AE strains being prevailed in Zhejiang province.

Methods: Gag fragments of the HIV-1 strains were amplified by nested-polymerase chain reaction (nPCR) from the DNA extracted from whole blood of HIV-1 infected individuals in Zhejiang province. PCR products were sequenced and analyzed by phylogenetic method.