Objective: Hypoxia-inducible factor 1alpha (HIF-1α) functions as a crucial transcriptional mediator in hypoxic and ischemic brain response. We endeavored to assess the prognostic significance of serum HIF-1α in human aneurysmal subarachnoid hemorrhage (aSAH).
Methods: In this prospective, longitudinal, multicenter, and observational study of 257 patients with aSAH and 100 healthy controls, serum HIF-1α levels were quantified.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
June 2007
Objective: To generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin.
Methods: We fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character.
Results: We therefore examined clones of PC3 cells stably expressing fusion gene and positive controlfluc with bioluminescence.