[Inhibitory effect of the recombinant adenovirus pre-miR-10a on K562 cell proliferation].

Aim: To investigate the changes of proliferation and apoptosis in K562 cells over-expressing miR-10a.

Methods: K562 cells were infected with pAd-pre-miR-10a virus, and then the levels of miR-10a and bcr/abl in transfected K562 cells was detected using RT-PCR and the level of BCR/ABL using Western blotting. The methylthiazolyl tetrazolium (MTT) assay and flow cytometry (FCM) were used to monitor the changes of proliferation and apoptosis of K562 cells after transfection.

Enhancement of specific cellular immune response induced by glycosyl-phosphatidylinositol-anchored BCR/ABL and mIL-12.

bcr/abl fusion gene is thought to be a promising target for chronic myelogenous leukemia (CML) patients to enhance immune response after attaining complete remission. In this study, we sought to enhance cellular immunity by co-expression of BCR/ABL and murine IL-12 gene on the tumor cell surface as a glycosyl-phosphatidylinositol (GPI)-form. The successfully constructed plasmid pBudCE4.