Agglutination of Helicobacter pylori coccoids by lectins.

AIM:To study the agglutination pattern of Helicobacter pylori coccoid andspiral forms.METHODS:Assays of agglutination and agglu-tination inhibition were applied using fifteen commercial lectins.RESULTS:Strong agglutination was observed with Mannose specific Concanavalin A(Con A), fucose specific Tetragonolobus purpureas(Lotus A) and N-acetyl glucosamine-specific Triticum vulgaris (WGA) lectins.

Coexistence of Helicobacter pylori spiral and coccoid forms in experimental mice.

AIM:To infect mice with Helicobacter pylori and detect immune response against two form of H. pylori.METHODS:An isolate of H.

Helicobacter pylori acquistion of metronidazole resistance by natural transformation in vitro.

AIM:To study whether Helicobacter pylori is naturally transformable.METHODS:Transformation was performed in BHI broth supplemented with horse serum and yeast extract. Genomic DNA extracted from a metronidazole resistant H.

Serum IgG response to differentiated antigens of Helicobacter pylori.

AIM:To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera.METHODS:Blood samples were collected from 278 patients with gastric diseases. A 3-day-old culture of H.