Publications by authors named "Jie-Ran Shi"
Article Synopsis
- The study investigates the effects of recombinant bacille Calmette-Guerin (rBCG) that expresses Der p2 on asthma, particularly its influence on Th17 cell differentiation and airway inflammation.
- Results showed that transferring dendritic cells infected with Der p2 rBCG led to reduced airway inflammation and mucin production, effectively inhibiting excessive Th17 responses compared to traditional BCG.
- The research highlights the importance of dendritic cells in rBCG's immunoregulatory functions and provides insight into the cellular mechanisms behind its potential as an asthma treatment.
Emerging evidence suggests that diffusion-weighted magnetic resonance imaging (DW MRI) could be useful for tumor detection with N and M staging of lung cancer in place of fluorine 18 fluorodeoxyglucose positron emission tomography/computed tomography (FDG PET/CT). DW MRI at 3.0 T and FDG PET/CT were performed before therapy in 113 patients with pulmonary nodules.
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- Researchers studied a recombinant bacille Calmette-Guérin (rBCG) vaccine expressing the Der p 2 allergen, which showed promise in reducing asthma symptoms by manipulating immune responses.
- The study aimed to investigate the role of dendritic cells (DCs) in this process and involved transferring DCs from vaccinated mice to other mice to assess their impact on airway inflammation.
- Results indicated that the transferred DCs significantly reduced asthmatic responses and promoted the development of regulatory T cells, suggesting that DCs are crucial for the rBCG's anti-asthma effects, especially through enhanced recruitment of certain DC subtypes.
Article Synopsis
- Allergic asthma is driven by T helper (Th)2 cell immune responses, and new immunotherapy strategies aim to shift these responses towards Th1 to alleviate symptoms.
- Previous studies demonstrated that a genetically modified form of bacille Calmette-Guerin (rBCG) could induce such a shift in naive mice, but its effectiveness in reducing allergic airway inflammation needed further investigation.
- The current research showed that rBCG significantly reduced airway inflammation and related symptoms in allergic mice by promoting a Th1 response and enhancing regulatory T cells that suppress Th2 cells, indicating its potential as an effective asthma treatment.
[Expression in E.coli and bioactivity assay of Micrococcus luteus resuscitation promoting factor domain and its mutants].
Zhonghua Jie He He Hu Xi Za Zhi
October 2008
Objective: To express Micrococcus luteus resuscitation promoting factor (Rpf) domain and its mutants in prokaryotic cells, and to investigate their bioactivity.
Methods: The gene of Rpf domain and its mutants (E54K, E54A) were amplified by polymerase chain reaction (PCR) from the genome of Micrococcus luteus and cloned into pMD18-T vector. After sequenced, the Rpf domain and its mutant gene were subcloned into expression vector PGEX-4T-1, and transfected into E.
[The effects of rBCG expressing Der p2 in the form of lipoprotein on murine immune response].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
May 2005
Aim: To investigate the effects of rBCG vaccination containing foreign antigen Der p2 in the form of lipoprotein on murine immune response.
Methods: 6 to 8 weeks old and newborn BALB/c mice were vaccined intraperitoneally with 10(6) CFU rBCG or BCG. At the same time, the control group was injected with saline.
[Using flow cytometry to detect peripheral blood eosinophils and their related molecules].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
July 2004
Aim: To set up an accurate and rapid method to detect the phenotype of peripheral blood eosinophils using flow cytometry.
Methods: 10(-4) mol/L of theophylline, 10(-4) of dexamethasone and 10(-8) mol/L of rhIL-5 were added to peripheral blood sampled from normal human subjects. Anti-CD16-PE mAb and FITC-labelled mAbs to eosinophil's molecules were used to perform double labeling staining.
[Construction and identification of the E.coli-BCG shuttle vector expressing lipoprotein Der p2 on cell wall of mycobacterium vaccae].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
March 2003
Aim: To construct the E.coli-BCG shuttle vector carrying and expressing dust mite antigen gene Der p2 on cell wall of mycobacterium vaccae.
Methods: The gene fragment encoding 19 kDa antigen and the upstream control element (19-ss) was amplified by PCR from the mycobacteria tuberculosis H37Rv.
[Constructing shuttle plasmid fusion expressing Ag85B-ESAT-6 on the surface of Mycobacterium].
Zhonghua Jie He He Hu Xi Za Zhi
April 2004
Objective: To construct the E. coli.-BCG (Bacille Calmette-Guerin) shuttle vector expressing Mycobacterium tuberculosis secreted protein Ag85B-ESAT-6 on the surface of Mycobacterium vaccae.
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