[Fourier transform infrared spectroscopy combined with attenuated total reflection applied to reagent-free quantitative analysis of thalassemia screening indicators].

Unlabelled: A simultaneous quantitative analysis method for the thalassemia screening indicators mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), and hemoglobin (Hb) was developed with Fourier transform infrared (FTIR) spectrometers and attenuated total reflection (ATR) combined with partial least squares (PLS). A total of 380 human peripheral blood samples were collected, which were composed of 180 positive samples and 200 negative samples according to the criteria of hematological indicator screening for thalassemia. One hundred fifty samples (64 negative, 86 positive) were randomly selected from all samples as the validation set, the remaining 230 samples (136 negative, 94 positive) were used as modeling samples; and then the modeling set was further subdivided into calibration set (68 negative, 47 positive, and 115 in total) and prediction set (68 negative, 47 positive, and 115 in total) for 200 times.