Molecular Insights Into the Sensory Adaption of the Cave-Dwelling Leech to the Karst Cave Environment.

Karst caves are a unique environment significantly different from the external environment; adaptation of cave-dwelling animals to the cave environment is often accompanied by shifts in the sensory systems. Aquatic and terrestrial leeches have been found in the karst caves. In this study, we conducted a transcriptome analysis on the cave-dwelling leech .

Assessing the possibility of using large language models in ocular surface diseases.

Aim: To assess the possibility of using different large language models (LLMs) in ocular surface diseases by selecting five different LLMS to test their accuracy in answering specialized questions related to ocular surface diseases: ChatGPT-4, ChatGPT-3.5, Claude 2, PaLM2, and SenseNova.

Methods: A group of experienced ophthalmology professors were asked to develop a 100-question single-choice question on ocular surface diseases designed to assess the performance of LLMs and human participants in answering ophthalmology specialty exam questions.

Rapid Generation of Reverse Genetics Systems for Coronavirus Research and High-Throughput Antiviral Screening Using Gibson DNA Assembly.

Coronaviruses (CoVs) pose a significant threat to human health, as demonstrated by the COVID-19 pandemic. The large size of the CoV genome (around 30 kb) represents a major obstacle to the development of reverse genetics systems, which are invaluable for basic research and antiviral drug screening. In this study, we established a rapid and convenient method for generating reverse genetic systems for various CoVs using a bacterial artificial chromosome (BAC) vector and Gibson DNA assembly.

Research on the Influence of Liquid Metal Embrittlement Cracks on the Strength and Fatigue Life of Spot-Welded Joints of Galvanized Q&P980 Steel.

Galvanized high-strength steel has emerged as a key focus in automotive lightweighting research. During resistance spot welding of galvanized steel, the phenomenon of liquid metal embrittlement (LME) can occur, which is characterized by the appearance of irregular cracks on the weld spot surface. However, the impact of LME cracks on the mechanical properties of joints remains unclear.

Prevalence and influencing factors of the dyadic psychological stress among patients with lung cancer and their family caregivers: a cross-sectional study.

Background: A lung cancer diagnosis has a huge impact on the psychological well-being of both patients and family caregivers. However, the current psychological stress status among dyads remains unclear. We aimed to determine the prevalence of anxiety and depression and identify the factors that influence patients with lung cancer and their caregivers.

Clinical Prognostic Impact of the Serum C-reactive Protein-to-albumin Ratio (CAR) in Chronic Heart Failure Patients: A Retrospective Study.

Background: The serum C-reactive protein-to-albumin ratio (CAR) has been identified as an adverse prognostic indicator in a variety of diseases. Nevertheless, there have been not been any studies reporting a relationship between CAR and the prognosis of chronic heart failure (CHF). This study was designed to evaluate the association between CAR and all-cause mortality in CHF patients with different ejection fractions.

Development of a dyadic mindfulness self-compassion intervention for patients with lung cancer and their family caregivers: A multi-method study.

Objective: Lung cancer and its prolonged treatment are profoundly unsettling for patients and their family caregivers, and developing dyadic measures to alleviate their negative affectivity is pivotal. This study aimed to develop a complex intervention to alleviate dyadic psychological stress among patients with lung cancer and their family caregivers.

Methods: A stepwise multi-method study was conducted following the Medical Research Council framework.

Investigating the efficacy of uncrosslinked porcine collagen coated vascular grafts for neointima formation and endothelialization.

Introduction: This study evaluates the efficacy of uncrosslinked porcine collagen coated vascular grafts (UPCCVG) in facilitating neointima formation and endothelialization.

Methods: Prior to coating, the uncrosslinked porcine collagen underwent comprehensive characterization employing SDS-PAGE, image analysis, circular dichroism and immunogenicity. The PET substrate of the vascular graft was coated with collagen solution utilizing the dip-coating method.

Prediction model for ocular metastasis of breast cancer: machine learning model development and interpretation study.

Background: Breast cancer (BC) is caused by the uncontrolled proliferation of breast epithelial cells followed by malignant transformation, and it has the highest incidence among female malignant tumors. The metastasis of BC occurs through direct and lymphatic spread. Although ocular metastasis is relatively rare, it is a good indicator of a worse prognosis.

Automatic detection of multi-antibiotic residues simultaneously in aquatic products by visual protein microarray chips.

A 96-well microplate-based protein microarray chip which can simultaneously detect three different families of antibiotics using a multiplexed approach has been developed for the first time. All steps in the microarray chip can be completed through an automated biochip analyzer, enabling high throughput analysis of 96 samples within 60 min according to the set program. The microarray chip can be visually evaluated by the color depth of array points, and can also be quantified through a scanner.

OCTA evaluates changes in retinal microvasculature in renal hypertension patients.

The objective of this study is to utilize optical coherence tomography angiography (OCTA) techniques for the purpose of identifying abnormalities in retinal and conjunctival vascular density among patients afflicted with renal hypertension. From October 2022 to October 2023, a cohort of sixteen patients diagnosed with renal hypertension (RH), comprising a total of 32 eyes, was selected from the Department of Nephrology at the First Affiliated Hospital of Nanchang University. Concurrently, a group of sixteen healthy individuals, carefully matched in characteristics, was recruited from volunteers at the Ophthalmology Research Center and designated as the healthy controls (HCs) group.

Transcriptome analysis to identify genes related to programmed cell death resulted from manipulating of BnaFAH ortholog by CRISPR/Cas9 in Brassica napus.

Fumarylacetoacetate hydrolase (FAH) catalyzes the final step of the tyrosine degradation pathway. In this study, we isolated and characterized two homologous BnaFAH genes in Brassica napus L. variant Westar, and then used CRISPR/Cas9-mediated targeted mutagenesis to generate a series of transgene-free mutant lines either with single or double-null bnafah alleles.

Microvascular alterations of the ocular surface and retina in connective tissue disease-related interstitial lung disease.

Aim: To examine the disparities in macular retinal vascular density between individuals with connective tissue disease-related interstitial lung disease (CTD-ILD) and healthy controls (HCs) by optical coherence tomography angiography (OCTA) and to investigate the changes in microvascular density in abnormal eyes.

Methods: For a retrospective case-control study, a total of 16 patients (32 eyes) diagnosed with CTD-ILD were selected as the ILD group. The 16 healthy volunteers with 32 eyes, matched in terms of age and sex with the patients, were recruited as control group.

Cross-cultural adaptation and reliability of the inventory of vicarious posttraumatic growth and research of its influencing factors: a cross-sectional study.

Objective: The purpose of this study was to translate the Vicarious Posttraumatic Growth Inventory (VPTGI) into Chinese and to assess its reliability and validity in Nurses, Additionally, it explored the correlations between vicarious posttraumatic growth (VPTG), Secondary Traumatic Stress (STS) and demographic variables.

Methods: The Brislin translation model was used to translate the VPTGI into Chinese. Validity analysis involved exploratory factor analysis (EFA), confirmatory factor analysis (CFA), and assessments of convergent validity, discriminant validity, and content validity.

Spatial resolved transcriptomics reveals distinct cross-talk between cancer cells and tumor-associated macrophages in intrahepatic cholangiocarcinoma.

Background: Multiple studies have shown that tumor-associated macrophages (TAMs) promote cancer initiation and progression. However, the reprogramming of macrophages in the tumor microenvironment (TME) and the cross-talk between TAMs and malignant subclones in intrahepatic cholangiocarcinoma (iCCA) has not been fully characterized, especially in a spatially resolved manner. Deciphering the spatial architecture of variable tissue cellular components in iCCA could contribute to the positional context of gene expression containing information pathological changes and cellular variability.

Structural Optimization of Polyimide-Film Humidity Sensors for New Energy Vehicles.

This paper presents a comprehensive study of the structural optimization of polyimide-film (PI-film) capacitive humidity sensors, with a focus on enhancing their performance for application in new energy vehicles (NEVs). Given the critical role of humidity sensors in ensuring the safety and efficiency of vehicle operations─particularly in monitoring lithium-ion battery systems─the study explores the intricate relationship between the interdigitated electrode (IDE) dimensions and the PI-film thickness to optimize sensor responsiveness and reliability. Through a combination of COMSOL Multiphysics simulations (a powerful finite element analysis, solver, and simulation software) and experimental validation, the research identifies the optimal geometrical combination that maximizes the sensitivity and minimizes the response time.

Comparison and use of explainable machine learning-based survival models for heart failure patients.

Objective: Explainable machine learning (XAI) was introduced in this study to improve the interpretability, explainability and transparency of the modelling results. The survex package in R was used to interpret and compare two survival models - the Cox proportional hazards regression (coxph) model and the random survival forest (rfsrc) model - and to estimate overall survival (OS) and its determinants in heart failure (HF) patients using these models.

Methods: We selected 1159 HF patients hospitalised at the First Affiliated Hospital of Kunming Medical University.

Sulfate Radical Based In Situ Vaccine Boosts Systemic Antitumor Immunity via Concurrent Activation of Necroptosis and STING Pathway.

In situ vaccine (ISV) can provoke systemic anti-tumor immunity through the induction of immunogenic cell death (ICD). The development of ISV technology has been restricted by the limited and suboptimal ICD driven tumor antigen production which are currently relying on chemo-drugs, photo-/radio-sensitizers, oncolytic-virus and immunostimulatory agents. Herein, a sulfate radical (SO ) based ISV is reported that accomplishes superior tumor immunotherapy dispense from conventional approaches.