Proteogenomic characterisation of primary oral cancer unveils extracellular matrix remodelling and immunosuppressive microenvironment linked to lymph node metastasis.

Oral squamous cell carcinoma (OSCC) is an increasingly prevalent malignancy worldwide. This study aims to understand molecular alterations associated with lymph node metastasis of OSCC in order to improve treatment strategies. We analysed a cohort of 46 patients with primary OSCC, including 10 with lymph node metastasis and 36 without.

Monitoring insecticide resistance and target-site mutations in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) in China.

The fall armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), a notorious invasive pest, has been widely monitored for insecticide resistance.

Modular Microfluidic Sensor Integrating Nucleic Acid Extraction, CRISPR/Cas13a, and Electrochemiluminescence for Multichannel RNA Detection.

Rapid and accurate screening of pathogens is crucial for disease detection. Here, a modular microfluidic sensor has been constructed for RNA detection, with integrated nucleic acid extraction, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a reaction, and electrochemiluminescence (ECL) detection. The sensor consists of nucleic acid processing and detection modules.

Transformation, bound-residue formation, translocation of tribromobisphenol A, 2,2'-dibromobisphenol A, 2,6-bromobisphenol A, and monobromobisphenol A in submerged soil and soil-rice seedling systems.

Tetrabromobisphenol A (TBBPA)-debrominated products has attracted growing concern in recent years. The sequential anoxic-oxic incubation approaches were effective in dissipation of environments containing halogenated xenobiotics. However, few studies focused on the submerged soil-plant systems, which appear common in environment.

Intraoperative Three-Dimensional Fluorescein Angiography-Guided Pars Plana Vitrectomy for the Treatment of Multiple Retinal Diseases.

Purpose: To explore the effectiveness and practicality of intraoperative fluorescein angiography (IOFA) in both common and rare retinal diseases.

Methods: We conducted a case series study involving three patients with vascular abnormality from various causes. A custom-designed, single-photon ultra-wideband spectral light source and integrated filters were utilized to perform IOFA.

Deciphering of differences in gut microbiota and plasma metabolites profile between non-obese and obese Golden Retrievers dogs.

Introduction: Golden Retrievers have a high risk of obesity, which is prevalent in dogs and is associated with inflammation and cancer, impairing the health and life expectancy of companion animals. Microbial and metabolite biomarkers have been proposed for identifying the presence of obesity in humans and rodents. However, the effects of obesity on the microbiome and metabolome of Golden Retrievers remains unknown.

Efficacy of Combined PD-1 Inhibitor and Bevacizumab in Unresectable Liver Metastasis of MSI-H Colorectal Cancer: A Case Report.

BACKGROUND Programmed death 1 (PD-1) inhibitors have demonstrated limited effectiveness in patients with microsatellite instability-high (MSI-H) colorectal cancer (CRC). Recent studies suggest that their efficacy can be enhanced when combined with anti-angiogenic agents. CASE REPORT We present a case of a 25-year-old woman with CRC harboring a KRAS mutation and MSI-H status, along with initially unresectable liver metastases.

METTL3 modulates colonic epithelium integrity via maintaining the self-renewal and differentiation of Lgr5+ stem cell.

The development and homeostasis of intestinal epithelium are mediated by actively proliferating Lgr5+ stem cells, which possess a remarkable self-renewal and differentiation capacity. Recently, our study demonstrated that m6A methylation was essential for the survival of colonic stem cells. Here, we show that METTL3 expression is downregulated in the colon mucosa in ulcerative colitis (UC) patients and strongly associated with the differentiation and maturation of goblet cells during inflammation.

Review on natural hydrogen wells safety.

Hydrogen is a promising clean energy source with geological reserves widely distributed globally, offering an annual flow exceeding 23 trillion grams. However, natural hydrogen extraction wells face unique safety challenges compared to conventional oil and gas wells. This paper reviews well safety concerns such as tubing/casing damage, cement/sealant failure, and excessive annular pressure buildup.

Individualised acute normovolaemic haemodilution for non-cardiac surgery with anticipated high-dose red cell transfusion: study protocol for a randomised controlled trial in West China Hospital of Sichuan University.

Introduction: Acute normovolaemic haemodilution (ANH) is a perioperative blood management technique involving the removal of whole blood and simultaneous infusion of colloids or crystalloids to achieve haemodilution while maintaining normovolaemia. However, its efficacy in reducing the requirement for perioperative allogeneic blood transfusion remains controversial due to inconsistent findings in the literature. An individualised red cell transfusion strategy, guided by the West China Liu's Score, has demonstrated effectiveness in reducing the need for allogeneic red cell transfusion.

Configurations associated with the efficiency of the ophthalmology departments in public hospitals of Central South China.

Background: Improving the efficiency of ophthalmology service is a global challenge to fight vision impairment, yet there is little concrete evidence of the current efficiency status. This study aimed to examine the efficiency of ophthalmology departments in the Hunan Province, China, and determine the associating factors of low-efficiency and high-efficiency ophthalmology departments.

Methods: This cross-sectional study included a province-level survey of ophthalmology departments of public hospitals.

Semi-supervised 3D retinal fluid segmentation via correlation mutual learning with global reasoning attention.

Accurate 3D segmentation of fluid lesions in optical coherence tomography (OCT) is crucial for the early diagnosis of diabetic macular edema (DME). However, higher-dimensional spatial complexity and limited annotated data present significant challenges for effective 3D lesion segmentation. To address these issues, we propose a novel semi-supervised strategy using a correlation mutual learning framework for segmenting 3D DME lesions from 3D OCT images.

Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis.

N-methylserotonin (NMS) is a valuable indole alkaloid with therapeutic potential for psychiatric and neurological disorders, and it is used in health foods, cosmetics, and weight loss supplements. However, environmental challenges and low reaction efficiencies significantly hinder cost-effective, large-scale production of NMS in plants or through chemical synthesis. Herein, we have successfully engineered Escherichia coli strains to enhance NMS production from L-tryptophan using whole-cell catalysis.

Red blood cell count and cystatin C as the specific biomarkers for diabetic retinopathy from diabetes mellitus: a case-control study.

Diabetic retinopathy (DR) is a common microvascular complication of diabetes mellitus (DM). However, the blood physiological indicators for this eye disease are less reported. Here, we systemically investigated healthy controls, DR patients and DM patients from 2014 to 2022 in Sichuan Provincial People's Hospital (Chengdu, China).

Design and synthesis of ()-3-benzylideneindolin-2-one derivatives as potential allosteric inhibitors of Aurora A kinase.

The mitotic kinase Aurora A, a pivotal regulator of the cell cycle, is overexpressed in various cancers and has emerged as one of the most promising targets for anticancer drug discovery. However, the lack of specificity and potential toxicity have impeded clinical trials involving orthosteric inhibitors. In this study, allosteric sites of Aurora A were predicted using the AlloReverse web server.

Microglial Lyzl4 Facilitates β-Amyloid Clearance in Alzheimer's Disease.

Alzheimer's Disease (AD) is a neurodegenerative condition characterized by the accumulation and deposition of amyloid-β (Aβ) aggregates in the brain. Despite a wealth of research on the toxicity of Aβ and its role in synaptic damage, the mechanisms facilitating Aβ clearance are not yet fully understood. However, microglia, the primary immune cells of the central nervous system, are known to maintain homeostasis through the phagocytic clearance of protein aggregates and cellular debris.

Heterogenous Chemistry of IO as a Critical Step in Iodine Cycling.

Global iodine emissions have been increasing rapidly in recent decades, further influencing the Earth's climate and human health. However, our incomplete understanding of the iodine chemical cycle, especially the fate of higher iodine oxides, introduces substantial uncertainties into atmospheric modeling. IO was previously deemed a "dead end" in iodine chemistry; however, we provide atomic-level evidence that IO can undergo rapid air-water or air-ice interfacial reactions within several picoseconds; these reactions are facilitated by prevalent chemicals on seawater such as amines and halide ions, to produce photolabile reactive iodine species such as HOI and IX (X = I, Br, and Cl).

Advances in Gas Detection of Pattern Recognition Algorithms for Chemiresistive Gas Sensor.

Gas detection and monitoring are critical to protect human health and safeguard the environment and ecosystems. Chemiresistive sensors are widely used in gas monitoring due to their ease of fabrication, high customizability, mechanical flexibility, and fast response time. However, with the rapid development of industrialization and technology, the main challenges faced by chemiresistive gas sensors are poor selectivity and insufficient anti-interference stability in complex application environments.

Promoting ClO Generation from the HOCl + HOCl Reaction on Aqueous/Frozen Air-Water Interfaces.

Hypochlorous acid (HOCl) is considered a temporary reservoir of dichlorine monoxide (ClO). Previous studies have suggested that ClO is difficult to generate from the reaction of HOCl + HOCl in the gas phase. Here, we demonstrate that ClO can be generated from the HOCl + HOCl reaction at aqueous/frozen air-water interfaces, which is confirmed by ab initio molecular dynamic calculations.