Publications by authors named "Jie Yun Zhuang"

Grain weight and grain shape are important traits that determine rice grain yield and quality. Mining more quantitative trait loci (QTLs) that control grain weight and shape will help to further improve the molecular regulatory network of rice grain development and provide gene resources for high-yield and high-quality rice varieties. In the present study, a QTL for grain length (GL) and grain width (GW), , was firstly fine-mapped into a 21.

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Grain weight and size, mostly determined by grain length, width and thickness, are crucial traits affecting grain quality and yield in rice. A quantitative trait locus controlling grain length and width in rice, qGS1-35.2, was previously fine-mapped in a 57.

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Article Synopsis
  • Grain shape and size significantly impact rice quality and yield, with a specific gene identified as a key determinant.
  • Knockout experiments revealed that the gene influences grain dimensions; its absence led to narrower grains and reduced weight, while overexpression resulted in notable improvements.
  • The study suggests that understanding this gene's regulatory role could enhance rice breeding for better grain appearance and quality.
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Grain weight and size are important traits determining grain yield and influencing grain quality in rice. In a previous study, a quantitative trait locus controlling thousand-grain weight (TGW) in rice, , was mapped in a 70.7 kb region on chromosome 10.

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Article Synopsis
  • A minor QTL for grain weight in rice, named qTGW1.2b, was identified and fine-mapped, revealing it falls within a 44.0 kb region.
  • The gene OsVQ4, responsible for the QTL's effect, was validated using CRISPR/Cas9-targeted mutagenesis, showing a greater impact on grain weight than initially expected.
  • The study demonstrates that CRISPR/Cas9 technology can effectively validate and manipulate minor QTLs in rice, paving the way for future genetic improvements by targeting small effect genes.
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Background: Grain weight is a grain yield component, which is an integrated index of grain length, width and thickness. They are controlled by a large number of quantitative trait loci (QTLs). Besides major QTLs, minor QTLs play an essential role.

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In rice, the contents of protein and amino acids are the major parameters of nutritional quality. Co-localization of quantitative trait loci (QTLs) for heading date and protein content were reported, but pleiotropism of heading-date genes on protein contents has not been investigated. Here, we reported that rice florigen gene plays an important role in controlling amino acid contents of rice grain.

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Rice is a major dietary source of essential mineral nutrients and toxic elements (aka ionome) for humans. However, the genetic basis underlying the variation in ionome is still largely unknown. Here, we mapped 51 and 61 quantitative trait loci (QTLs) controlling the concentrations of 13 and 15 elements in rice (Oryza sativa L.

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Article Synopsis
  • The study focuses on identifying specific QTL (quantitative trait loci) that influence grain size traits, which are crucial for both yield and quality in rice.
  • Researchers used 12 different rice populations to analyze and narrow down the location of a previously mapped QTL on chromosome 10 that affects grain weight, length, and width.
  • The results identified three distinct QTLs within the target region, with varying impacts on the traits, indicating a complex genetic network that regulates grain size in rice.
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Appropriate flowering time is crucial for successful grain production, which relies on not only the action of individual heading date genes, but also the gene-by-gene interactions. In this study, influences of interaction between and on flowering time and yield traits were analyzed using near isogenic lines derived from a cross between rice cultivars ZS97 and MY46. In the non-functional background, the functional allele promoted flowering under both the natural short-day (NSD) conditions and natural long-day (NLD) conditions.

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Background: Grain size is a key determinant of grain weight and a trait having critical influence on grain quality in rice. While increasing evidences are shown for the importance of minor-effect QTL in controlling complex traits, the attention has not been given to grain size until recently. In previous studies, five QTL having small effects for grain size were resolved on the long arm of chromosome 1 using populations derived from indica rice cross Zhenshan 97///Zhenshan 97//Zhenshan 97/Milyang 46.

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Background: Rice is highly sensitive to temperature fluctuations. Recently, the frequent occurrence of high temperature stress has heavily influenced rice production. Proper heading date in specific environmental conditions could ensure high grain yield.

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Background: Heavy metal accumulation in rice is a growing concern for public health. Backcross inbred lines derived from an interspecific cross of Oryza sativa × O. rufipogon were grown in two distinct ecological locations (Hangzhou and Lingshui, China).

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Important role of flowering genes in enhancing grain productivity in rice has become well recognized for a number of key genes regulating the florigen production, but little has been known for the two florigen genes themselves. In this study, pleiotropism of Rice Flowering Locus T 1 (RFT1), one of the two florigen genes in rice, was firstly evaluated using near isogenic lines (NILs) carrying RFT1 alleles from the indica rice cultivars Zhenshan 97 (ZS97) and Milyang 46, respectively, and then determined by transformation of the RFT1 allele into a japonica rice variety, Zhonghua 11. The RFT1 allele was shown to delay heading and increase plant height, grain weight, grain number and grain yield, indicating that RFT1 plays an important role in the growth and development of rice.

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Analysis of the genetic basis of yield heterosis in rice was conducted by quantitative trait locus mapping using a set of 204 recombinant inbred lines (RILs), its testcross population, and mid-parent heterosis dataset (H). A total of 39 QTLs for six yield traits were detected, of which three were detected in all the datasets, ten were common to the RIL and testcross populations, six were common to the testcross and H, and 17, 2, and 1 were detected for RILs, testcrosses, and H, respectively. When a QTL was detected in both the RIL and testcross populations, the difference between TQ and IR24 and that between Zh9A/TQ and Zh9A/IR24 were always in the same direction, providing the potential to increase the yield of hybrids by increasing the yield of parental lines.

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The role of photoperiod sensitivity (PS) of flowering genes have become well recognized in rice, whereas little attention has been drawn to the non-PS component of these genes, especially to their influence on gene-by-gene interactions. Rice populations in which the photoperiod-sensitive allele at Hd1 has become insensitive to photoperiod but continued to affect heading date (HD) were used in this study to fine-map a quantitative trait locus (QTL) for HD and analyze its genetic relationship to Hd1 The QTL was delimitated to a 96.3-kb region on the distal end of the long arm of chromosome 7.

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Article Synopsis
  • This study focuses on mapping minor quantitative trait loci (QTLs) related to grain weight and size in rice, building on previous research that identified major QTLs.
  • Using near isogenic lines (NILs) derived from four selected BC2F10 plants, researchers analyzed the effects of two identified QTLs (qTGW1.1a and qTGW1.1b) across different environmental conditions, finding their impact on traits like 1000-grain weight, grain length, and grain width.
  • The results showed that qTGW1.1a, influenced by the ZS97 allele, primarily affects grain weight, while qTGW1.1b,
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Macro- and microelement contents are important traits for nutritional quality in rice. In this study, quantitative trait loci (QTLs) for the contents of seven mineral elements in milled rice were detected using recombinant inbred lines (RILs) of the indica rice cross Zhenshan 97/Milyang 46, followed by the validation and fine mapping of a QTL region on the short arm of chromosome 6. A total of 20 QTLs distributed on chromosomes 1, 3, 5, 6, 10, and 11 were detected in the RIL population.

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A minor QTL for heading date located on the long arm of rice chromosome 1 was delimitated to a 95.0-kb region using near isogenic lines with sequential segregating regions. Heading date and grain yield are two key factors determining the commercial potential of a rice variety.

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Article Synopsis
  • Five populations of near isogenic lines (NILs) were created from a cross between Zhenshan97 and Milyang 46 to study the impact of the Hd1 gene on heading date, height, and yield in rice.
  • The ZS97 allele of Hd1 showed different responses to photoperiods, promoting flowering in short days while delaying it in long days, unlike the MY46 allele which consistently delayed flowering and improved yield traits.
  • The study highlighted the significant additive effects of these alleles on various traits, suggesting that the photoperiod-insensitive allele from MY46 has potential for breeding high-yield rice varieties, particularly in low latitude regions.
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Rice straw is always regarded as a by-product of rice production, but it could be a significant energy source for ruminant animals. Knowledge of the genetic variation and genetic architecture of cell wall traits will facilitate rice breeders by improving relevant traits through selective breeding and genetic engineering. The common wild rice, Oryza rufipogon Griff.

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Validation and dissection of a QTL region for leaf traits in rice which has been reported in a number of independent studies were conducted. Three sets of near isogenic lines (NILs) were originated from a residual heterozygous line derived the indica cross Zhenshan 97B/Milyang 46. They were overlapping and totally covered a 4.

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Article Synopsis
  • The study utilized a population of 247 recombination inbreed lines (RILs) from an indica cross to create a linkage map with 250 DNA markers for QTL mapping related to plant height and root traits under both normal and salt stress conditions.
  • Six QTLs linked to the studied traits were identified under salt stress, explaining 6.01% to 16.50% of the phenotypic variation, while six additional QTLs were found under normal conditions, with variations ranging from 4.39% to 18.50%.
  • Significant QTLs affecting root length and relative water content were located in specific intervals on chromosomes 1 and 3, indicating overlapping genetic influences.
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Background: China has been successful in breeding hybrid rice strains, but is now facing challenges to develop new hybrids with high-yielding potential, better grain quality, and tolerance to biotic and abiotic stresses. This paper reviews the most significant advances in hybrid rice breeding in China, and presents a recent study on fine-mapping quantitative trait loci (QTLs) for yield traits.

Scope: By exploiting new types of male sterility, hybrid rice production in China has become more diversified.

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Article Synopsis
  • Researchers created two cDNA libraries from rice lines that have similar genetics and resistance to blast disease, focusing on their different responses to neck blast.
  • They identified 90 unique cDNA clones, with 74 having known functions related to pathogen response and signaling based on database comparisons.
  • RT-PCR confirmed expression differences in 17 of 26 selected cDNA clones, with this study being the first to examine neck blast resistance at the mRNA level, paving the way for deeper genetic research.
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