Publications by authors named "Jie Wen Douglas Tay"
Article Synopsis
- Researchers fused inactive Cas13 proteins with a modified human ADAR2 deaminase to create a new method for precise RNA editing without causing permanent changes in the genome.
- The new platform, called xPERT, was engineered to improve the balance between effective on-target editing and reduced off-target activity, addressing a major concern in existing RNA-editing technologies.
- Compared to previous systems (REPAIRv1 and REPAIRv2), xPERT demonstrates strong on-target editing while minimizing unintended alterations, making it a potentially safer option for customizing RNA and protein functions.
Extensive testing is essential to break the transmission of SARS-CoV-2, which causes the ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is robust to viral genome mutations and temperature, produces results fast, can be applied directly on nasopharyngeal (NP) specimens without RNA purification, and incorporates a human internal control within the same reaction. Specifically, we show that the use of an engineered AsCas12a enzyme enables detection of wildtype and mutated SARS-CoV-2 and allows us to perform the detection step with loop-mediated isothermal amplification (LAMP) at 60-65 °C.
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