Early-warning ecological risk assessments of multi-element pollution in the surface soil of karst basins in southwest China based on the delayed geochemical hazard model.

Soil potentially toxic element (PTE) pollution, especially in karst regions, poses significant ecological risks due to the unique geological features and environmental conditions. This study focuses on the delayed geochemical hazard (DGH) model to assess the progressive risks of cadmium (Cd) and lead (Pb) contamination in the surface soils of karst regions in southwestern China. The study found that Pb and Cd pollution in karst areas presents ecological risks, with the region's high porosity and alkaline soils facilitating the transformation of pollutants from stable to mobile forms.

CRISPR/Cas9-mediated mutagenesis of SEED FATTY ACID REDUCER genes significantly increased seed oil content in soybean.

Increasing seed oil content (SOC) is an important breeding goal for soybean breeding. While significant efforts have been made to improve SOC through metabolic pathway engineering, research to increase soybean SOC by reducing lipid degradation and fatty acid (FA) decomposition during seed maturation process is limited. Seed Fatty Acid Reducers (SFAR) are members of the GDSL enzyme family and play a crucial role in lipid metabolism.

A case report of neuronal intranuclear inclusion disease and literature review.

Neuronal intranuclear inclusion disease (NIID) is a rare progressive neurodegenerative disease with a characteristic pathological feature of eosinophilic hyaluronan inclusions in the nervous system and internal organs. The identification of GGC-repeat expansions in the Notch 2 N-terminal like C (NOTCH2NLC) gene facilitates the accurate diagnosis of NIID. Due to its rareness and high clinical heterogeneity, the diagnosis of NIID is often delayed or missed.

The Benefits of Virtual Reality Travel on Symptom Burden and Mental Health of Hemodialysis Patients.

Background: . Hemodialysis (HD) patients often face a heavy symptom burden, low subjective well-being, and high perceived stress. Traditional non-pharmacological interventions have limited effectiveness in improving this situation.

Subarachnoid anesthesia for sacrococcygeal pilonidal disease treatment: A case report.

Rationale: Sacrococcygeal pilonidal disease (SPD) is a chronic inflammatory condition primarily affecting young males. This case report details the perioperative anesthetic management of a patient undergoing SPD surgery under subarachnoid anesthesia.

Patient Concerns: A 48-year-old obese male (body mass index 28 kg/m2) presented with recurrent sacrococcygeal swelling, pain, and purulent discharge for 2 months.

Zscan4 mediates ubiquitination and degradation of the corepressor complex to promote chromatin accessibility in 2C-like cells.

Zygotic genome activation occurs in two-cell (2C) embryos, and a 2C-like state is also activated in sporadic (~1%) naïve embryonic stem cells in mice. Elevated chromatin accessibility is critical for the 2C-like state to occur, yet the underlying molecular mechanisms remain elusive. Zscan4 exhibits burst expression in 2C embryos and 2C-like cells.

Global Meta-Analysis of Individual and Combined Nitrogen Inhibitors: Enhancing Plant Productivity and Reducing Environmental Losses.

Nitrogen (N) transformation inhibitors have been widely recognized as a promising strategy to enhance crop productivity and mitigate N losses. However, the effectiveness of individual or combined inhibitors can vary significantly across different agroecosystems. Using meta-analysis and cost-benefit analysis (CBA), we synthesized findings from 41 peer-reviewed studies (285 observations) globally to evaluate the efficacy of urease inhibitors (UIs), nitrification inhibitors (NIs), and combined inhibitors (UINIs).

Roles of gut microbiome-associated metabolites in pulmonary fibrosis by integrated analysis.

Lung diseases often coincide with imbalances in gut microbiota, but the role of gut microbiota in pulmonary fibrosis (PF) remains unclear. This study investigates the impact of gut microbiota and their metabolites on PF. Serum and lung tissues of normal, bleomycin (BLM)- and silica-induced mice showed significant differences in gut microbiota.

Comfort-based design of summer green space optimization in arid oasis-type cities.

Arid, oasis-type cities significantly limit the quality and duration of outdoor activities for residents due to their hot, dry summer climate. This study aims to enhance environmental comfort in dry, hot regions, focusing on the outdoor environment of a densely populated university in Xinjiang. Through on-site research and field measurements, the thermal baseline and key factors influencing comfort across different times and locations were analyzed.

Radiotherapy-immunomodulated nanoplatform triggers both hypoxic and normoxic tumor associated antigens generation for robust abscopal effect and sustained immune memory.

Radiotherapy (RT) induced abscopal effect has garnered substantial attention, nevertheless, it is rarely observed in clinics, due to the tumor hypoxia-related radioresistance, inadequate immune stimulation, and immunosuppressive tumor microenvironment. Herein, we construct a radiotherapy-immunomodulated nanoplatform (THUNDER), which synergizes with RT and greatly triggers the generation of both hypoxic and normoxic tumor cells-derived tumor-associated antigens (TAAs), resulting in robust abscopal effect and sustained immune memory. THUNDER exhibits prolonged blood circulation and high tumor retention capacity.

Neddylation drives myofibrillogenesis in the developing heart.

Neddylation is a highly conserved post-translational modification that plays critical roles in various cellular processes through the modulation of cullins and non-cullin substrates. While neddylation is known to be essential for embryonic development, tumor growth, and organogenesis of different tissues, its role in cardiogenesis remains unexplored. Here, we investigated the role of neddylation in early cardiac development by deleting the gene encoding a regulatory subunit of the NEDD8-specific E1 activating enzyme, Nae1, globally and in a heart-specific fashion via Nkx2-5.

High-Entropy and Na-Rich-Designed High-Energy-Density NaV(PO)/C Cathode.

The NaV(PO) (NVP) cathode holds the merit of a stable 3D NASICON structure for ultrafast Na diffusion, yet it is still confronted with poor electronic conductivity (10 S cm) and insufficient energy density (∼370 W h kg). Herein, a series of high-entropy-doped NaVZn(GaCrAlIn)(PO) ( = 0, 0.2, 0.

O-GlcNAcylation-related genes mediate tumor microenvironment characteristics and prediction of immunotherapy response in gastric cancer.

We aim to identify molecular clusters related to O-GlcNAcylation and establish a novel scoring system for predicting prognosis and immunotherapy efficacy in patients with gastric cancer (GC). The transcriptomic and clinical data are obtained from XENA-UCSC and GEO databases. The O-GlcNAcylation-related genes are obtained from the GSEA database.

N-methyladenosine on the natural antisense transcript of NIA1 stabilizes its mRNA to boost NO biosynthesis and modulate stomatal movement.

Nitric oxide (NO) is a crucial signaling molecule that regulates a wide range of metabolic pathways in different strata of organisms. In plants, nitrate reductase (NR) is a key enzyme for NO biosynthesis. There are two NR-encoding genes in Arabidopsis, NIA1 and NIA2, which are precisely regulated and expressed in a tissue-specific manner.

Post-transplant complications revealed by mycophenolate mofetil related transporters and metabolic enzymes gene polymorphisms in pediatric patients with hematological disorders.

Background: Haploidentical hematopoietic stem cell transplantation (Haplo-HSCT) serves as an important option for patients without an HLA matched donor in treating hematological disorders, while patients may experience various complications after transplantation. Mycophenolate mofetil (MMF), a cornerstone drug for graft-versus-host disease (GvHD) prophylaxis, effectively reduces the incidence of acute GvHD, and the efficacy of MMF varies among individuals associated with MMF-related transporters and metabolic enzymes single nucleotide polymorphisms (SNPs). However, limited studies have systematically reported the correlations between the MMF-related SNPs and post-transplant complications.

Role of N6-methyladenosine methylation in nasopharyngeal carcinoma: current insights and future prospective.

Nasopharyngeal carcinoma (NPC) is a distinct type of head and neck squamous cell carcinoma prevalent in Southern China, Southeast Asia, and North Africa. Despite advances in treatment options, the prognosis for advanced NPC remains poor, underscoring the urgent need to explore its underlying mechanisms and develop novel therapeutic strategies. Epigenetic alterations have been shown to play a key role in NPC progression.

The impact of a low-calorie, reduced-fat diet on liver attenuation imaging: a randomized clinical trial.

Purpose: To investigate whether a low-calorie, reduced-fat diet affects liver attenuation imaging (ATI) measurements.

Methods: A total of 320 participants were enrolled in this prospective study. They were randomly assigned to four groups: a fasting group, a postprandial 0.

Patient-reported functional outcomes and treatment-related regret in Hispanic and Spanish-speaking men following prostate cancer treatment.

Objectives: Compare functional outcomes and treatment-related regret over 10 years in Spanish- and English-speaking Hispanic men compared to non-Hispanic men following treatment of localized prostate cancer.

Methods And Materials: Data from a prospective cohort study of men with localized prostate cancer treated with active surveillance, radical prostatectomy or radiotherapy were used to examine the effect of survey language (Spanish speaking vs. English speaking) and ethnicity (Hispanic vs.

Disulfide-Directed Multicyclic Peptides with N-Terminally Extendable α-Helices for Recognition and Activation of G Protein-Coupled Receptors.

Many peptide hormones adopt long α-helical structures upon interacting with their cognate receptors but often exhibit flexible conformations when unbound. Strategies that can stabilize long α-helices without disrupting their binding to receptors are still lacking, which hinders progress in their biological applications and drug development. Here, we present an approach that combines rational design with library screening to create and identify a unique disulfide-directed multicyclic peptide (DDMP) scaffold, which could effectively stabilize N-terminally extendable α-helices while displaying exceptional efficiency in disulfide pairing and oxidative folding.

Permeability-Engineered Compartmentation System-Enabled Digital PCR (PECS-dPCR): A Digital Platform toward Multistep Biomolecular Assays.

Droplet-based digital PCR has emerged as a powerful platform for nucleic acid-based detection. However, the formation of droplet compartments and the subsequent amplification process in oil present significant drawbacks: instability under harsh thermal conditions, high background fluorescent noise inside droplets, and major difficulty in supporting multistep assays. Alternatively, droplets made of a hydrogel, or other advanced materials, have been adopted and demonstrate promising improvement over conventional droplet-based platforms.

KDM4 Regulates the Glycolysis of Hemocytes in the Immune Priming of .

Immune priming confers a sustained, augmented response of innate immune cells to a secondary challenge, a process that is characteristically reliant on metabolic reprogramming. Recent evidence suggests that histone demethylases play essential roles in the immune priming, while its regulation role in the metabolic reprogramming remains largely unknown. In the present study, the concentration of glucose was significantly down-regulated in the hemocytes of crab after secondary stimulation with , while the expression levels of phosphofructokinase (PFK) pyruvate kinase (PK), hexokinase-2 (HK-2) and Glucose-6-phosphate dehydrogenase (G-6-PD), along with the concentrations of lactate and the ratio of NAD/NADH, were elevated.

Advanced PROTAC and Quantitative Proteomics Strategy Reveals Bax Inhibitor-1 as a Critical Target of Icaritin in Burkitt Lymphoma.

Understanding the molecular targets of natural products is crucial for elucidating their mechanisms of action, mitigating toxicity, and uncovering potential therapeutic pathways. Icaritin (ICT), a bioactive flavonoid, demonstrates significant anti-tumor activity but lacks defined molecular targets. This study employs an advanced strategy integrating proteolysis targeting chimera (PROTAC) technology with quantitative proteomics to identify ICT's key targets.

High Prevalence of 5'UTR Mutations in Anaplastic Thyroid Cancer.

Anaplastic thyroid cancer (ATC) is a rare but one of the most lethal types of human cancer. Although increasing evidence demonstrated that ATC tumors had a high mutation burden, little is known about the aberrancy of the noncoding genome of ATC except the well-investigated () promoter mutations. The mutational statuses of 5' untranslated region (5'UTR), intron 6, and promoters, as well as the promoter and mutations were determined using Sanger sequencing in 28 patients with ATC (19 women and 9 men) with a median (interquartile range) age of 64 (55-71) years, 14 thyroid cancer cell lines and a normal thyroid cell line.

Mechanism of action of curcumin targeting TRPM2/NLRP3 signaling axis to mediate cell death in the treatment of knee osteoarthritis.

Objects: This study intends to explore the possible mechanisms of curcumin's action after knee osteoarthritis.

Methods: Chondrocytes alone were used to mimic the cellular inflammatory response with interleukin IL-1β. Overexpressing TRPM2 chondrocytes were constructed using cell transfection technique for mechanism verification.

Case report: A case of giant malignant solitary fibrous tumor of the pleura with Doege-Potter's syndrome and review of the literature.

The solitary fibrous tumor of the pleura (SFTP) is a rare intrathoracic neoplasm that commonly originates from the subpleural mesenchymal cells of the visceral pleura and accounts for less than 5% of all pleural tumors. We reported a case of a 54-year-old man with a two-week history of hypoglycemia, a six-month history of productive cough and fatigue, and chronic right chest pain. Radiological techniques revealed a giant intra-thoracic mass with hypervascularization, and pathological staining was carried out to make a definitive diagnosis of SFTP.