Celastrol increases osteosarcoma cell lysis by γδ T cells through up-regulation of death receptors.

γδ T cells has been shown to exhibit profound antitumor effects in a broad range of tumor entities, including OS. However, resistance to γδ T cells is a serious problem in the management of OS. This study investigates the impact of celastrol on the expression of death receptors 4/5 (DR4/5) on OS cell lines (HOS, U2OS) and cancer cell lysis by γδ T cells.

[Transplantation of collagen-chitosan nerve conduits filled with glial cell line-derived neurotrophic factor gene-modified schwann cells for the repair of sciatic nerve defect].

Objective: To explore the effects of the transplantation of collagen-chitosan nerve scaffold containing glial cell line-derived neurotrophic factor(GDNF)gene modified schwann cells on the recovery of long-distance sciatic nerve defect.

Methods: The rat models of 8 mm long-distance sciatic nerve defect were established and divided into three groups, with 6 rats in each group. In GDNF-Sch group, the defect was repaired by GDNF modified Schwann cells combined with collagen-chitosan nerve scaffold.

Solitary haemangioma of the shaft of long bones: resection and reconstruction with autologous bone graft.

Bone haemangiomas are uncommon lesions, occurring in the skull or spine. A solitary haemangioma in the diaphysis of a long bone is rare. We retrospectively investigated six patients who presented with a solitary haemangioma in a long bone diaphysis.

[Interferon-γ enhances human γδ T cell-mediated osteosarcoma cell killing in vitro].

Objective: To investigate the cytotoxic effect of γδ T cells from osteosarcoma patients against interferon-γ (IFN-γ)-treated osteosarcoma cells in vitro.

Methods: Human γδ T cells were amplified by zoledronate from peripheral blood cells of osteosarcoma patients. The expression of Fas on the osteosarcoma cells were measured by flow cytometry and quantitative real-time PCR analysis before and after IFN-γ treatment.

[Effect of zoledronate on the cytotoxicity of γδ T cells from PBMCs of osteosarcoma patients against osteosarcoma].

Objective To investigate the effect of zoledronate (Zol) on the cytotoxicity of γδ T cells from peripheral blood mononuclear cells (PBMCs) of osteosarcoma (OS) patients against OS. Methods γδ T cells, derived from PBMCs of patients with primary, recurrent and metastatic OS, were ex vivo expanded by Zol and IL-2. γδ T cell cytotoxicity against target cells was analyzed by a standard lactate dehydrogenase (LDH) release assay.

Comparing the chondrogenic potential in vivo of autogeneic mesenchymal stem cells derived from different tissues.

Objective: to compare the chondrogenic ability of mesenchymal stem cells (MSCs) derived from different tissues in rabbits' full-thickness articular cartilage defects.

Methods: sixty New Zealand white rabbits of ordinary grade with a body weight of 2.5 approximately 3.

In vitro and in vivo biocompatibility investigation of diamond-like carbon coated nickel-titanium shape memory alloy.

Objective: To investigate the biocompatibility of diamond-like carbon (DLC) coated nickel-titanium shape memory alloy (NiTi SMA) in vitro and in vivo.

Methods: The in vitro study was carried out by co-culturing the DLC coated and uncoated NiTi SMA with bone marrow mesenchymal stem cells (MSCs), respectively, and the in vivo study was carried out by fixing the rabbits' femoral fracture model by DLC coated and uncoated NiTi SMA embracing fixator for 4 weeks, respectively. The concentration of the cells, alkaline phosphatase (AKP), and nickel ion in culture media were detected, respectively, at the first to fifth day after co-culturing.

[Effect factors analysis of knee function recovery after distal femoral fracture operation].

Objective: To investigate the effect factors of knee function recovery after operation in distal femoral fractures.

Methods: From January 2001 to May 2007, 92 cases of distal femoral fracture were treated. There were 50 males and 42 females, aged 20-77 years old (average 46.

Nervus cutaneus femoris posterior pedicle flap for repairing large soft tissue defects at the heel or inferior segment of the shank.

Objective: To investigate the clinical effect of the nervus cutaneus femoris posterior pedicle flap on repairing large soft tissue defects at the heel or inferior segment of the shank.

Methods: Totally 14 cases were followed up for 8-22 months (mean 15.5 months) to observe the clinical effects of nervus cutaneus femoris posterior pedicle flap on repairing large soft tissue defects of the heel or inferior segment of the shank.

[Strategy of the treatment for dislocation of cervical vertebra].

Objective: To study the strategy of the treatment for dislocation of cervical vertebra.

Methods: The clinical data of 39 cases with dislocation of cervical vertebra were analyzed. Among them,29 were male and 10 were female.