The effect of no naked pancreatic surface in the cavity of jejunum on pancreaticojejunostomy in 132 consecutive cases.

Background/aims: To prevent the pancreatic fistulas, we designed a technique termed "no naked pancreatic surface in the cavity of jejunum" on pancreaticojejunostomy.

Methodology: We adopted pancreatic exocrine secretions following the pancreatic duct by drainage; there was no naked pancreatic surface in the cavity of jejunum, and entail 2-3 cm sheath of the jejunum to the pancreatic stump.

Results: Only 3 (2.

MicroRNA‑122 regulation of the morphology and cytoarchitecture of hepatoma carcinoma cells.

MicroRNAs (miRNAs) are a large family of post‑transcriptional regulators of gene expression that control a number of developmental and cellular processes in eukaryotic organisms and are ~23 nucleotides in length. miRNA‑122 is an abundant liver‑specific miRNA, implicated in fatty acid and cholesterol metabolism, as well as in hepatitis C viral replication and is frequently suppressed in primary hepatocellular carcinomas. In the current study, the Hep3B cell line with stable overexpression of miR‑122 was successfully established through gene transfection methods and drug screening.

Effect of OSW-1 on microRNA expression profiles of hepatoma cells and functions of novel microRNAs.

3β,16β,17α-trihydroxycholest-5-en-22-one 16-O-(2-O-4-methoxybenzoyl-β-D-xylopyranosyl)-(1→3)- (2-O-acet​yl-α-L-arabinopyranoside) (OSW-1) is a member of the cholestane saponin family, which was first isolated from the bulbs of Ornithogalum saundersiae and previously reported to be cytotoxic against several types of malignant cells. However, its antitumor mechanism remains unclear. Therefore, we investigated microRNA (miRNA) expression profiles in order to explore the antitumor activities of OSW-1.

Signaling network of OSW‑1‑induced apoptosis and necroptosis in hepatocellular carcinoma.

The compound 3β, 16β, 17α‑trihydroxycholest‑5‑en‑22‑one 16‑O‑(2‑O‑4‑methoxybenzoyl‑β‑D‑xylopyranosyl)‑ (1→3)‑(2‑O‑acetyl‑α‑L‑arabinopyranoside (OSW‑1) is a member of the cholestane saponin family that was created in the bulbs of Ornithogalum saudersiae. OSW‑1 has previously been shown as cytotoxic against numerous types of malignant cells, however, its antitumoral mechanisms remain unclear. The present study aimed to examine the potential changes in the gene expression of a hepatocellular carcinoma (HCC) cell line (Hep3B) incubated with OSW‑1 in vitro.