Selenenylsulfide covalent-directed chemistry for the detection of sulfhydryl groups using a diselenide fluorescent probe.

We report the development of a diglycosyldiselenide-based fluorescent probe for the rapid detection of sulfhydryl-containing biomolecules. The probe facilitates a chemoselective coupling reaction with sulfhydryl groups in aqueous buffer under ambient conditions, resulting in the formation of homogeneous Se-S conjugates within one hour. Using glutathione, a sulfhydryl-containing biomolecule, as a proof of concept, the probe achieved a detection limit of 0.

Qualifying P-glycoprotein in drug-resistant ovarian cancer cells: a dual-mode aptamer probe approach.

Drug resistance presents a significant obstacle in treating human ovarian cancer. The development of effective methods for detecting drug-resistant cancer cells is pivotal for tailoring personalized therapies and prognostic assessments. In this investigation, we introduce a dual-mode detection technique employing a fluorogenic aptamer probe for the qualification of P-glycoprotein (P-gp) in drug-resistant ovarian cancer cells.

A Novel Baseline-Free Method for Damage Localization Using Guided Waves Based on Hyperbola Imaging Algorithm.

Most imaging methods based on ultrasonic Lamb waves in structural health monitoring requires reference signals, recorded in the intact state. This paper focuses on a novel baseline-free method for damage localization using Lamb waves based on a hyperbolic algorithm. This method employs a special array with a relatively small number of transducers and only one branch of the hyperbola.

Effect of the internet combined with exercise-based individualized nursing intervention in patients with gestational diabetes mellitus.

Background: Gestational diabetes mellitus (GDM) is the most frequent medical complication of pregnancy. This condition is manifested by glucose intolerance resulting in hyperglycemia of variable severity during pregnancy. One of the most important clinical tools for efficiently regulating maternal blood glucose is strictly monitoring blood glucose levels.

[Professor 's clinical experience of acupuncture for oculomotor nerve paralysis].

Professor -'s clinical experience of acupuncture for oculomotor nerve palsy was summarized and one typical case was introduced. Professor skillfully applied traditional acupuncture theory and classical acupuncture method, and proposed a diagnosis and treatment system combining meridian syndrome differentiation and specific acupuncture techniques. In addition, Professor combined the -primary and -connecting acupoints combination method, theory of "removing the stagnation of blood and " and theory of root-stem and origin-end, etc.

Sensitive Carbon Monoxide Gas Sensor Based on Chemiluminescence on Nano-Au/NdO-CaNdO: Working Condition Optimization by Response Surface Methodology.

An Au/NdO-CaNdO composite was synthesized by the sol-gel and impregnation method. The EDS spectrum and the transmission electron microscopy image showed that Au atoms are uniformly distributed on the surface of NdO-CaNdO with a size of less than 50 nm. A sensitive carbon monoxide gas sensor based on chemiluminescence at a temperature lower than 200 °C was reported.

Collection and disinfection of forensic biological specimens in five cases concerning COVID-19 in Guangzhou, China.

There have been many cases of pneumonia caused by novel coronavirus infections in China and around the world. This will inevitably lead to a rise in the number of patients. At the present time, clinical and forensic autopsies have given guidance and explanations in relation to the problem of COVID-19 transmission and defense.

New method for large-scale facial skin sebum quantification and skin type classification.

Background: The demand for personalized skin care is increasing rapidly in recent years. In order to provide suitable products for different customers, an accurate assessment of the skin condition is required. It is necessary to establish a remote and convenient method for quantifying skin condition.

Lamb Wave-Based Structural Health Monitoring on Composite Bolted Joints under Tensile Load.

Online and offline monitoring of composite bolted joints under tensile load were investigated using piezoelectric transducers. The relationships between Lamb wave signals, pre-tightening force, the applied tensile load, as well as the failure modes were investigated. Results indicated that / wave amplitudes decrease with the increasing of load.