Worldwide used bio-insecticides Cry1Ac toxin has no detrimental effects on Episyrphus balteatus but alter the symbiotic microbial communities.

Hoverflies, capable of abilities providing dual ecosystem services including pest control and pollination, are exposed to insecticidal proteins from transgenic plants via pollen and prey aphids. However, the effects of such exposures on hoverflies have never been adequately assessed. Here, we investigated impacts of the most widely used biotoxin Cry1Ac on a representative hoverfly species Episyrphus balteatus through food chain transmission and active toxin exposure.

Life table analysis and RNA-Seq reveal hormesis and transgenerational effects of deltamethrin on Aphis gossypii.

Background: Deltamethrin, as a highly effective and broad-spectrum insecticide, has been widely used for agricultural pest control such as Aphis gossypii worldwide. Increasing evidence has shown that despite great economic benefits brought by it, deltamethrin has also non-negligible side effects. However, the potential risks and related molecular mechanisms remain largely unclear.

Effects of developmental stages, sex difference, and diet types of the host marmalade hoverfly ( on symbiotic bacteria.

Introduction: Symbiotic bacteria play key roles in a variety of important life processes of insects such as development, reproduction and environmental adaptation, and the elucidation of symbiont population structure and dynamics is crucial for revealing the underlying regulatory mechanisms. The marmalade hoverfly () is not only a remarkable aphid predator, but also a worldwide pollinator second to honeybees. However, its symbiont composition and dynamics remain unclear.

Chromosome-level genome assembly of cotton thrips Thrips tabaci (Thysanoptera: Thripidae).

Cotton thrip, Thrips tabaci is a major polyphagous pest widely distributed on a variety of crops around the world, causing huge economic losses to agricultural production. Due to its biological and genomic characteristics, this pest can reproduce quickly and develop resistance to various pesticides in a very short time. However, the lack of high-quality reference genomes has hindered deeper gene function exploration and slows down the development of new management strategies.

Chromosome-level genome assembly of marmalade hoverfly Episyrphus balteatus (Diptera: Syrphidae).

Episyrphus balteatus can provide dual ecosystem services including pest control and pollination, which the larvae are excellent predators of aphid pest whereas adults are efficient pollinator. In this study, we assembled a high-quality genome of E. balteatus from northern China geographical population at the chromosome level by using Illumina, PacBio long reads, and Hi-C technologies.

Integrated Omics Analysis Reveals Key Pathways in Cotton Defense against Mirid Bug ( Jakovlev) Feeding.

The recent dominance of Jakovlev as the primary cotton field pest in Bt-cotton-cultivated areas has generated significant interest in cotton pest control research. This study addresses the limited understanding of cotton defense mechanisms triggered by feeding. Utilizing LC-QTOF-MS, we analyzed cotton metabolomic changes induced by , and identified 496 differential positive ions (374 upregulated, 122 downregulated) across 11 categories, such as terpenoids, alkaloids, phenylpropanoids, flavonoids, isoflavones, etc.

Sublethal acetamiprid affects reproduction, development and disrupts gene expression in Binodoxys communis.

At present, understanding of neonicotinoid toxicity in arthropods remains limited. We here evaluated the lethal and sublethal effects of acetamiprid in F and F generations of Binodoxys communis using a range of sublethal concentrations. The 10% lethal concentration (LC) and half lethal concentration (LC) of ACE had negative effects on the B.

Thripidae pest species community identification and population genetic diversity analyses of 2 dominant thrips in cotton fields of China.

Thrips are devastating pests for various crops, and they can rasp tender leaves, terminal buds, and flowers, which specifically causing huge economic losses to cotton production. However, there is very little knowledge about the species composition of thrips in mainland China, as well as the genetic structure of the thrips populations, particularly in the cotton-producing regions. In this study, thrips were collected from 40 geographical locations at 8 different provinces which representing majority cotton-producing belts in China, and mitochondrial cytochrome oxidase subunit I sequence was used to identify species composition and evaluate the genetic diversity of collected thrips individuals.

Effects of sublethal fipronil exposure on cross-generational functional responses and gene expression in Binodoxys communis.

The effective systemic insecticide fipronil is widely used on a variety of crops and in public spaces to control insect pests. Binodoxys communis (Gahan) (Hymenoptera: Braconidae) is the dominant natural enemy of Aphis gossypii Glover (Homoptera: Aphididae), an important cotton pest, and has good efficiency in inhibiting aphid populations. The direct effects of environmental residues of sublethal fipronil doses on adult B.

Response of the Microbiota to Treatment with Cry1B Protein.

(Thunberg) (Coleoptera: Coccinellidae) is a dominant natural enemy of insect pests in farmland ecosystems. It also serves as an important non-target insect for environmental safety evaluations of transgenic crops. Widespread planting of transgenic crops may result in direct or indirect exposure of to recombinant (Bt) protein, which may in turn affect the biological performance of this natural enemy by affecting the microflora.

Silencing the rhythm gene AgCLK-1 reduced feeding of Aphis gossypii.

The cotton aphid Aphis gossypii Glover is an important cotton pest, and means of controlling this insect is a primary research focus. Although biological rhythm is an important mechanism that regulates numerous insect processes and activities, its role in cotton aphid has not been elucidated. In the present study, four highly-expressed circadian rhythm genes were selected from the cotton aphid genome database and their physicochemical properties and protein structures were analyzed.

Comparative transcriptome and proteome reveal the unique genes and proteins of female parasitic wasps, Lysiphlebia japonica Ashmead.

Background: Lysiphlebia japonica Ashmead (Hymenoptera, Braconidae) is an endophagous parasitoid wasp and its host, Aphis gossypii Glover (Hemiptera, Aphididae) is a major cotton pest. L. japonica affects the growth and fatty acid metabolism of cotton aphids after parasitization and has been widely used as a biocontrol agent.

Assessment of the risk of imidaclothiz to the dominant aphid parasitoid Binodoxys communis (Hymenoptera: Braconidae).

The neonicotinoid of imidaclothiz insecticide with low resistance and high efficiency, has great potential for application in pest control in specifically cotton field. In this systematically evaluate the effects of sublethal doses of imidaclothiz (LC: 11.48 mg/L; LC: 28.

Glyphosate decreases survival, increases fecundity, and alters the microbiome of the natural predator Harmonia axyridis (ladybird beetle).

Glyphosate is a widely-used herbicide that shows toxicity to non-target organisms. The predatory natural enemy Harmonia axyridis may ingest glyphosate present in pollen and aphid prey. The present study characterized the responses of adult H.

Chromosome level genome assembly of oriental armyworm Mythimna separata.

The oriental armyworm, Mythimna separata, is an extremely destructive polyphagous pest with a broad host range that seriously threatens the safety of agricultural production. Here, a high-quality chromosome-level genome was assembled using Illumina, PacBio HiFi long sequencing, and Hi-C scaffolding technologies. The genome size was 706.

Sexually dimorphic morphology, feeding behavior and gene expression profiles in cotton aphid Aphis gossypii.

Background: Sexual dimorphism exists in most insects; however, less is known about sexual dimorphism in aphids. In this study, we identified sexually dimorphic differences in morphology, feeding behavior and gene expression between sexual females and males of the cotton aphid through electron microscopy, electrical penetration graph techniques and RNA sequencing.

Results: All males were alate with a slender reddish-yellow body and abdominal yellow-black stripes, whereas all sexual females were apterous with a pudgy green body.

Sublethal toxicity of sulfoxaflor to parasitoid Binodoxys communis Gahan.

Integrated pest management is focused on combining biological and chemical controls. There is evidence of a negative impact of neonicotinoids on biological control, however, sulfoxaflor (SFX), a novel insecticide, its impact on parasitoid natural predator remain limited. Binodoxys communis is an important parasitic natural enemy of Aphis gossypii, which may have direct and indirect toxicity from the insecticides and aphids.

Risk assessment of predatory lady beetle Propylea japonica's multi-generational exposure to three non-insecticidal agrochemicals.

The effects of non-insecticidal agrochemicals on pest natural predators remain largely unexplored except bees and silkworm. The herbicide quizalofop-p-ethyl (QpE), fungicide thiophanate-methyl (TM), and plant growth regulator mepiquat chloride (MC) have been extensively applied as non-insecticidal agrochemicals. Here, we systematically evaluated multiple effects of these 3 non-insecticidal agrochemicals on three generations of Propylea japonica, an important agroforestry predatory beetle, including the effects on its development, reproduction, enterobacteria, and transcriptomic response.

Parasitization of Glover by Gahan Causes Shifts in the Ovarian Bacterial Microbiota.

Background: Glover is an important agricultural pest distributed worldwide. Gahan is the main parasitoid wasp of . Previous studies have shown that parasitization causes reduced egg production in , but the effects of parasitism on the symbiotic bacteria in the host ovaries are unknown.

Response of the Pardosa astrigera bacterial community to Cry1B protein.

While genetically modified (GM) crops bring economic benefits to human beings, their impact on non-target organisms has become an important part of environmental safety assessments. Symbiotic bacteria play an important role in eukaryotic biological functions and can adjust host communities to adapt to new environments. Therefore, this study examined the effects of Cry1B protein on the growth and development of non-target natural enemies of Pardosa astrigera (L.

breaks the specialization of the cotton-specialized aphid () by providing nutrition through zucchini.

The cotton aphid, Glover, is a species of polyphagous aphid with many biotypes, and its host transfer has always been the focus of research on the control of cotton aphid. An important factor affecting aphid specialization is the nutritional association with microbial symbionts that provide the host with nutrients lacking in the diet. We analyzed the microbial composition and biodiversity of reared on zucchini for 10 generations (T1-T10) and cotton as a control (CK), by high-throughput Illumina sequencing of 16S ribosomal RNA genes.

Exposure to flupyradifurone affect health of biocontrol parasitoid Binodoxys communis (Hymenoptera: Braconidae) via disrupting detoxification metabolism and lipid synthesis.

Assessing the potential effects of insecticides on beneficial biological control agents is key to facilitating the success of integrated pest management (IPM) approaches. Flupyradifurone (FPF) is a novel neonicotinoid insecticide that is replacing traditional neonicotinoids over a large geographical range to control pests. Binodoxys communis, is the dominant parasitic natural enemy of aphids.