Cell-Cycle-Related and Expression Elevated Protein in Tumor Upregulates the Antioxidant Genes via Activation of NF-κB/Nrf2 in Acute Liver Injury.

Background And Aims: Cell-cycle-related and expression elevated protein in tumor (CREPT, also named RPRD1B) is highly expressed in tumors and functions to promote tumorigenesis. However, the role of CREPT in the pathophysiology of acute liver injury is limited. Here, we demonstrate that CREPT plays an essential role during acute liver injury.

Characterizing populations prioritized for PrEP in 19 African countries: a review of national guidance.

Introduction: While African countries have expanded access to HIV pre-exposure prophylaxis (PrEP) since 2015, regional targets for PrEP uptake remain unmet. Understanding which populations are prioritized for PrEP at the policy level is an important step in determining the scope of PrEP distribution across Africa and identifying gaps in programme implementation. We reviewed national guidance to characterize populations prioritized for PrEP in Africa.

Causal Association Between Endometriosis and Coronary Heart Disease: Evidence from the National Health and Nutrition Examination Survey and Mendelian Randomization Analysis.

Objective: Failure to resolve inflammation is a novel feature of angiogenic-dependent diseases such as endometriosis and atherosclerosis.The correlation and causality between endometriosis and coronary heart disease is unclear. Here, we investigated the correlation and causality between endometriosis and coronary heart disease.

Material Cycles, Environmental Emissions, and Ecological Risks of Bisphenol A (BPA) in China and Implications for Sustainable Plastic Management.

Bisphenol A (BPA) is a high-production-volume plastic chemical, with ∼98% of its usage in China allocated to producing polycarbonate and epoxy resin, and its fugitive release threatens ecosystems. However, knowledge of its anthropogenic cycles, environmental emissions, and ecological risks remains incomplete, hindering effective plastic lifecycle management. Herein, material flow analysis, multimedia environmental modeling, and ecological risk assessment were integrated to comprehensively map BPA dynamics in China.

Ultrasound-assisted extraction of polysaccharide from Allium chinense G. Don epidermal waste: Evaluation of extraction mechanism, physicochemical properties, and bioactivities.

Allium chinense G. Don waste (ACGD) has an abundance of polysaccharides (ACGDP). Therefore, in this study, a method for extraction of ACGDP from ACGD by ultrasonic-assisted hot water extraction (UAE) based on ultrasonic cleaning equipment was developed.

Exosome-derived proteins in gastric cancer progression, drug resistance, and immune response.

Gastric cancer (GC) represents a prevalent malignancy globally, often diagnosed at advanced stages owing to subtle early symptoms, resulting in a poor prognosis. Exosomes are extracellular nano-sized vesicles and are secreted by various cells. Mounting evidence indicates that exosomes contain a wide range of molecules, such as DNA, RNA, lipids, and proteins, and play crucial roles in multiple cancers including GC.

Transcriptome analysis and functional identification of transfer RNA-derived fragments in grape leaves exposed to UV-C radiation.

Transfer RNA-derived fragments (tRFs) are noncoding small RNAs derived from transfer RNAs (tRNAs) in microorganisms, animals and plants. In plants, tRFs are known to respond to environmental stimuli, including heat, oxidative stress and UV radiation; however, their specific functions in horticultural plants, such as grapevine, remain poorly understood. In this study, we used RNA-seq to identify differentially expressed genes (DEGs) in grape leaves exposed to UV-C radiation.

Deep learning model for low-dose CT late iodine enhancement imaging and extracellular volume quantification.

Objectives: To develop and validate deep learning (DL)-models that denoise late iodine enhancement (LIE) images and enable accurate extracellular volume (ECV) quantification.

Methods: This study retrospectively included patients with chest discomfort who underwent CT myocardial perfusion + CT angiography + LIE from two hospitals. Two DL models, residual dense network (RDN) and conditional generative adversarial network (cGAN), were developed and validated.

FOLR2 macrophage depletion from intestinal metaplasia to early gastric cancer: single-cell sequencing insight into gastric cancer progression.

Background: The immune landscape associated with different subtypes of intestinal metaplasia (IM) and early gastric cancer (EGC) remains unclear. This study aimed to investigate the immune landscape of complete intestinal metaplasia (CIM), incomplete intestinal metaplasia (IIM), and EGC, as well as the underlying mechanisms of EGC progression.

Methods: Gastric biopsy samples were collected from five patients with CIM, six patients with IIM, and four patients with EGC, followed by single-cell RNA sequencing.

RAB37 suppresses the EMT, migration and invasion of gastric cancer cells by mediating autophagic degradation of β-catenin.

Background: Gastric cancer, characterized by its high morbidity and mortality rates, exhibits low levels of RAB37. The role and molecular mechanisms of RAB37, a small GTPase, in the pathogenesis of gastric cancer are still unclear.

Methods: We assessed RAB37 expression in gastric cancer cells using quantitative Polymerase Chain Reaction (qPCR), Western blot, and immunohistochemical staining (IHC), and analyzed EMT marker proteins and autophagy changes via Western blot, immunofluorescence (IF), and transmission electron microscopy (TEM).

Profiling the gut structure and microbiota, and identifying two dominant bacteria belonging to the genus in mandarin fish () fed an artificial diet.

Mandarin fish () fed with an artificial diet is progressively gaining popularity, which is important for reducing product prices and resource consumption. However, food is the decisive factor of intestinal microbes, and the profound effects of change in their feeding habit on intestinal microbes of mandarin fish have not been revealed. In the present study, live bait fish and artificial diet were used to feed mandarin fish for 8 weeks to study the effect of different feeding habits on the histology, microbiota structure and dominant bacteria of gut in mandarin fish.

The relative effects of climatic drivers and phenotypic integration on phenotypic plasticity of a globally invasive plant.

Introduction: Understanding the constraints of phenotypic plasticity can provide insights into the factors that limit or influence the capacity of an organism to respond to changing environments. However, the relative effects of external and internal factors on phenotypic plasticity remain largely unexplored. Phenotypic integration, the pattern of correlations among traits, is recognized as an important internal constraint to plasticity.

Characterization of a phage 4FS1 and its depolymerase.

The significant economic losses caused by in donkey husbandry have increased interest in exploring the potential of phages and their enzymes as control strategies. In this study, a phage, designated 4FS1, was isolated from sewage at a donkey farm. Transmission electron microscopy (TEM) revealed a typical icosahedral head and a long, non-contractile tail.

Manganese nanosheets loaded with selenium and gemcitabine activate the tumor microenvironment to enhance anti-tumor immunity.

Breast cancer is among the most common malignant tumors globally. Despite advances in immunotherapy and targeted therapies, chemotherapy remains the primary clinical treatment. Gemcitabine, a cytosine nucleoside analog, is widely used for various solid tumors; however, its effectiveness is often limited by drug resistance and adverse side effects.

Adenocarcinoma of the rete testis: clinicopathological study of 18 cases with emphasis on MET amplification and a review of the literature.

Background: Knowledge regarding adenocarcinoma of the rete testis (ACRT) is extremely limited due to its scarcity.

Methods And Results: This study enrolled 18 patients with ACRT from multiple institutions. Clinicopathological and immunohistochemical features were investigated, together with a comprehensive review of 95 previously reported cases.

Downregulated KLF4, induced by m6A modification, aggravates intestinal barrier dysfunction in inflammatory bowel disease.

Background: Krüppel-like factor 4 (KLF4), a transcription factor, is involved in various biological processes. However, the role of KLF4 in regulating the intestinal epithelial barrier (IEB) in inflammatory bowel disease (IBD) and its mechanism have not been extensively studied.

Methods: KLF4 expression in IBD patients and colitis mice was analyzed using Gene Expression Omnibus(GEO) database, immunohistochemistry (IHC) and Western blot.

Efficient purification and excitation energy transfer characterization of phycoerythrin 545 from Rhodomonas sp.

Cryptomonad phycoerythrin 545 (PE545) is an important type of phycobiliprotein in basic research and technological innovations. Herein, we report a minimalistic hydrophobic chromatography method for its purification. High purity was achieved, with a purity ratio (A/A) of 13.

Non-lethal sonodynamic therapy inhibits high glucose and palmitate-induced macrophage inflammasome activation through mtROS-DRP1-mitophagy pathway.

Obesity plays a crucial role in the development and progression of type 2 diabetes mellitus (T2DM) by causing excessive release of free fatty acid from adipose tissue, which in turn leads to systemic infiltration of macrophages. In individuals with T2DM, the infiltration of macrophages into pancreatic islets results in islet inflammation that impairs beta cell function, as evidenced by increased apoptosis and decreased glucose-stimulated insulin secretion. The present study aimed to investigate the effects of non-lethal sonodynamic therapy (NL-SDT) on bone marrow-derived macrophages (BMDMs) exposed to high glucose and palmitic acid (HG/PA).

Everolimus treatment in patients with hormone receptor-positive and human epidermal growth factor receptor 2-negative advanced breast cancer and a predictive model for its efficacy: a multicenter real-world study.

Background: Everolimus is beneficial for patients with hormone receptor-positive and human epidermal growth factor receptor 2-negative (HR+/HER2-) advanced breast cancer (ABC). However, some patients developed drug resistance and the well-established predictor for everolimus efficacy was limited.

Objectives: The study was designed to evaluate the efficacy of everolimus in different treatment lines and identify several clinicopathological markers to estimate everolimus efficacy in patients with HR+/HER2- ABC.

Dephosphorylation of bZIP59 by PP2A ensures appropriate shade avoidance response in Arabidopsis.

Changes in light quality and quantity experienced by many shade-intolerant plants grown in close proximity lead to transcriptional reprogramming and shade avoidance syndrome (SAS). Despite the importance of phosphorylation-dependent signaling in cellular physiology, phosphorylation events during SAS are largely unknown. Here, we examined shade-regulated phosphorylation events in Arabidopsis using quantitative phosphoproteomics.

Synergistic in vivo anticancer effects of 1,7-heptanediol and doxorubicin co-loadedliposomes in highly aggressive breast cancer.

Breast cancer holds the highest incidence rate among women. Doxorubicin (DOX) is a potent frontline drug for the treatment of breast cancer. The anticancer mechanisms of DOX include inducing immunogenic cell death in tumor cells, causing damage to tumor DNA, and generating free radicals.

Predicting Fatigue Damage in Hydrogels Through Force-Induced Luminescence Enhancement.

Fatigue damage of polymers occurs under long-term load cycling, resulting in irreversible fracture failure, which is difficult to predict. The real-time monitoring of material fatigue damage is of great significance. Here, tough hydrogels are prepared with force-induced confined luminescence enhancement of carbonated polymer quantum dot (CPD) clusters to realize the visualization of fracture process and the monitoring of fatigue damage.