Synthesis of Thiohydantoin Scaffolds on DNA for Focused DNA-Encoded Library Construction.

Thiohydantoin represents a significant class of biologically active privileged heterocyclic scaffolds. Herein, we present a convenient and robust DNA-compatible method for constructing a thiohydantoin-focused DNA-encoded library. This reaction can be applied to a wide variety of isothiocyanate partners, arylamine feedstocks, and diverse α-amine acid derivatives, exhibiting excellent conversions, high functional group tolerance, and preservation of DNA tag integrity.

Passive Vision Detection of Torch Pose in Swing Arc Narrow Gap Welding.

To enhance the synchronous detection of the horizontal and vertical positions of the torch in swing arc narrow gap welding, a torch pose detection (TPD) method is proposed. This approach utilizes passive visual sensing to capture images of the arc on the groove sidewall, using advanced image processing methods to extract and fit the arc contour. The coordinates of the arc contour center point and the highest point are determined through the arc contour fitting line.

Microstructure and Hardness Characteristics of Swing-Arc SAW Hardfacing Layers.

Hot-rolled backup rolls are widely used in steel rolling and usually need to be repaired by arc hardfacing after becoming worn. However, a corrugated-groove defect commonly occurs on the roll surface due to the uneven hardness distribution in the hardfacing layers, affecting the proper usage of the roll. Accordingly, a new swing-arc submerged arc welding (SA-SAW) process is proposed to attempt to solve this drawback.

Krüppel-like factor 10 protects against metabolic dysfunction-associated steatohepatitis by regulating HNF4α-mediated metabolic pathways.

Background: Krüppel-like factor 10 (KLF10), a zinc finger transcription factor, plays a pivotal role in modulating TGF-β-mediated cellular processes such as growth, apoptosis, and differentiation. Recent studies have implicated KLF10 in regulating lipid metabolism and glucose homeostasis. This study aimed to elucidate the precise role of hepatic KLF10 in developing metabolic dysfunction-associated steatohepatitis (MASH) in diet-induced obese mice.

Study of Hygrothermal Aging for Basalt Fiber/Epoxy Resin Composites Modified with CeCl.

With increasing attention being paid to environmental issues, the application of natural fibers in fiber-reinforced composites has attracted more and more attention. Composite materials with basalt fibers (BFs) as reinforcement have excellent properties and are widely used in many fields. Hydrothermal aging crucially influences the durability of basalt fiber/epoxy resin composites (BF/ERCs).

Hepatic FOXA3 overexpression prevents Western diet-induced obesity and MASH through TGR5.

Forkhead transcription factor 3 (FOXA3) has been shown to regulate metabolism and development. Hepatic FOXA3 is reduced in obesity and fatty liver disease. However, the role of hepatic FOXA3 in regulating obesity or steatohepatitis remains to be investigated.

QTL-seq analysis identified the genomic regions of plant height and days to heading in high-latitude rice.

Rice ( L.) is one of the most extensive crops in the world. China's Heilongjiang Province is the northernmost rice-growing region in the world.

Shikonin triggers GSDME-mediated pyroptosis in tumours by regulating autophagy via the ROS-MAPK14/p38α axis.

Background: Shikonin (SK), a botanical drug extracted from Lithospermum erythrorhizon, has been shown to inhibit tumour growth through apoptosis and necrosis. However, whether SK induces pyroptosis in cancer cells is still unknown.

Purpose: This study aims to investigated the mechanisms of SK-induced pyroptosis in tumour cells and mice.

Low level light therapy/photobiomodulation for diabetic peripheral neuropathy: protocol of a systematic review and meta-analysis.

Introduction: Diabetic peripheral neuropathy (DPN) is one of the most common complications of diabetes that strongly impact the patients' quality of life and working ability. Evidence indicated that low level light therapy (LLLT)/photobiomodulation might be effective for neuropathy. However, the effect of LLLT for DPN is not clear.

Downregulation of microRNA-124-3p promotes subventricular zone neural stem cell activation by enhancing the function of BDNF downstream pathways after traumatic brain injury in adult rats.

Aims: In this study, the effect of intracerebral ventricle injection with a miR-124-3p agomir or antagomir on prognosis and on subventricular zone (SVZ) neural stem cells (NSCs) in adult rats with moderate traumatic brain injury (TBI) was investigated.

Methods: Model rats with moderate controlled cortical impact (CCI) were established and verified as described previously. The dynamic changes in miR-124-3p and the status of NSCs in the SVZ were analyzed.

Infrared Visual Sensing Detection of Groove Width for Swing Arc Narrow Gap Welding.

To solve the current problem of poor weld formation due to groove width variation in swing arc narrow gap welding, an infrared passive visual sensing detection approach was developed in this work to measure groove width under intense welding interferences. This approach, called global pattern recognition, includes self-adaptive positioning of the ROI window, equal division thresholding and in situ dynamic clustering algorithms. Accordingly, the self-adaptive positioning method filters several of the nearest values of the arc's highest point of the vertical coordinate and groove's same-side edge position to determine the origin coordinates of the ROI window; the equal division thresholding algorithm then divides and processes the ROI window image to extract the groove edge and forms a raw data distribution of groove width in the data window.

Clinical and Epidemiological Study of Intracranial Tumors in Children and Identification of Diagnostic Biomarkers for the Most Common Tumor Subtype and Their Relationship with the Immune Microenvironment Through Bioinformatics Analysis.

Brain tumors are the second most common pediatric malignancy and have poor prognosis. Understanding the pathogenesis of tumors at the molecular level is essential for clinical treatment. We conducted a retrospective study on the epidemiology of brain tumors in children based on clinical data obtained from a neurosurgical center.

A Consumer Segmentation Study of Nutrition Information Seeking and Its Relation to Food Consumption in Beijing, China.

The aim of this study is to identify consumer groups based on nutrition information-seeking behavior and how it relates to food consumption. Although the Chinese public can now access nutrition information through different channels, research on the segmentation of homogeneous consumer groups seeking nutrition information is lacking. This study closes this research gap and, in doing so, also shows how information seeking is related to dietary behavior.

Biochemical and Structural Characterization of a Novel Bacterial Tannase From in Ruminant Gastrointestinal Tract.

Tannases are a family of esterases that catalyze the hydrolysis of ester and depside bonds present in hydrolyzable tannins to release gallic acid. Here, a novel tannase from (TanA) was characterized. The recombinant TanA exhibited maximal activity at pH 7.

Molecular mechanism of shikonin inhibiting tumor growth and potential application in cancer treatment.

Shikonin is one of the major bioactive components of . It has a good killing effect in a variety of tumor cells. Its antitumor effect involves multiple targets and pathways and has received extensive attention and study in recent years.

Comprehensive Effect of Arc and Ultrasonic Energy on MIG Arc Ultrasonic Welding.

Ultrasonic energy is introduced into the Metal Inert Gas (MIG) welding arc and weld pool by superposition of an ultrasonic frequency current. In this study, the arc shape, arc energy, and ultrasonic energy that responded to ultrasonic excitation voltage and frequency is investigated. The comprehensive influence of arc and ultrasonic energy on weld formation, microstructure, and mechanical properties is further studied.

Mobility speed predicts new-onset hypertension: a longitudinal study.

Objective: The aim of this study was to investigate whether declining mobility and muscle strength predict new-onset hypertension in suburban-dwelling elderly individuals.

Methods: This study was designed as a longitudinal prospective cohort study. It was comprised of 362 individuals (mean age = 67.

Opposing and Operated Side Electroacupuncture Generates Similar Analgesic Effects on Pain after Knee Surgery.

The purpose of this study was to investigate whether opposing electroacupuncture (EA) could produce similar analgesic effects as operated side EA after knee surgery in rats. Sprague Dawley rats were randomly divided into the sham surgery group, and three surgery groups: opposing EA, operated side EA, and model. After surgery, compared with the sham surgery group, three kinds of pain behavior test methods (mechanical withdrawal threshold (MWT), cumulative pain score [CPS], and mechanical hypersensitivity of knee) were used to assess the pain behavior of the rats in the surgery groups.

Identification of key methylation differentially expressed genes in posterior fossa ependymoma based on epigenomic and transcriptome analysis.

Background: Posterior fossa ependymoma (EPN-PF) can be classified into Group A posterior fossa ependymoma (EPN-PFA) and Group B posterior fossa ependymoma (EPN-PFB) according to DNA CpG island methylation profile status and gene expression. EPN-PFA usually occurs in children younger than 5 years and has a poor prognosis.

Methods: Using epigenome and transcriptome microarray data, a multi-component weighted gene co-expression network analysis (WGCNA) was used to systematically identify the hub genes of EPN-PF.

Molecular mechanism of liver X receptors in cancer therapeutics.

Liver X receptors (LXRs) are receptors that belong to the nuclear receptor superfamily (NRs). It was originally called the "orphan receptor" when it was firstly discovered. Then it was found to be activated by oxysterol and it was officially named LXRs.

Peptidoglycan recognition protein-S1 acts as a receptor to activate AMP expression through the IMD pathway in the silkworm Bombyx mori.

Peptidoglycan recognition proteins (PGRPs) are the most important pattern recognition receptors (PRRs) in insects. PGRPs can recognize pathogenic microorganism peptidoglycans (PGs) and play an important role in innate immunity. Twelve PGRPs have been identified in silkworms.

Liver X receptor activation reduces gastric cancer cell proliferation by suppressing Wnt signalling via LXRβ relocalization.

Liver X receptors (LXRs) are involved in various diseases associated with lipid disorders, and in regulating cancer cell proliferation. However, the underlying molecular mechanisms, especially those in gastric cancer (GC) remain to be clarified. In this study, immunohistochemistry analysis revealed that LXRβ was mainly expressed in GC tissue, with less expression in adjacent normal tissues.

Immunogenic cell death in anticancer chemotherapy and its impact on clinical studies.

The traditional view holds that apoptosis is non-immunogenic and does not induce an inflammatory response. However, recent studies have suggested that certain chemotherapeutic drugs that induce tumor cell apoptosis can induce immunogenic cell death (ICD) in cancer cells. This process is characterized by not only up-regulation of a series of signaling molecules in cancer cells, including expose of calreticulin (CRT), secretion of adenosine triphosphate (ATP) and release of high mobility group box 1 (HMGB1).

Hepatic Knockdown of Splicing Regulator Slu7 Ameliorates Inflammation and Attenuates Liver Injury in Ethanol-Fed Mice.

Aberrant precursor mRNA splicing plays a pivotal role in liver diseases. However, roles of splicing regulators in alcoholic liver disease are unknown. Herein, we investigated a splicing regulator, Slu7, in the development of alcoholic steatohepatitis.