Regulatory significance of terminator: A systematic approach for dissecting terminator-mediated enhancement of upstream mRNA stability.

The primary function of terminators is to terminate transcription in gene expression. Although some studies have suggested that terminators also contribute positively to upstream gene expression, the extent and underlying mechanism of this effect remain largely unexplored. Here, the correlation between terminating strength and upstream mRNA stability was investigated by constructing a terminator mutation library through randomizing 5 nucleotides, assisted by FlowSeq technology, terminator variants were categorized based on the downstream fluorescence intensity, followed by high-throughput sequencing.

Increased global posterior tibial slope is significantly associated with higher ACL graft signal intensity on 2-Year postoperative MRI after primary ACL reconstruction using hamstring tendon autografts.

Background: The healing and remodeling process of tendon grafts after anterior cruciate ligament (ACL) reconstruction (ACLR) has received significant attention. This study aims to identify preoperative factors associated with postoperative signal intensity of the ACL graft after primary ACLR.

Methods: A total of 90 patients underwent primary ACLR using hamstring tendon autografts by the same senior surgeon between January 2013 and December 2020 were included.

Emotional differences based on comments on doctor-patient disputes with varying levels of severity.

Background: The risks associated with negative doctor-patient relationships have seriously hindered the healthy development of medical and healthcare and aroused widespread concern in society. The number of public comments on doctor-patient relationship risk events reflects the degree to which the public pays attention to such events.

Aim: To explore public emotional differences, the intensity of comments, and the positions represented at different levels of doctor-patient disputes.

Using to control rice planthopper populations: progress and challenges.

have been developed as a tool for protecting humans from mosquito populations and mosquito-borne diseases. The success of using relies on the facts that are maternally transmitted and that -induced cytoplasmic incompatibility provides a selective advantage to infected over uninfected females, ensuring that rapidly spread through the target pest population. Most transinfected exhibit a strong antiviral response in novel hosts, thus making it an extremely efficient technique.

Respiratory Fluid Mechanics of the Effect of Mouth Breathing on High-Arched Palate: Computational Fluid Dynamics Analyses.

Computational fluid dynamics (CFD) was introduced into the study of palate growth and development to explain the mechanisms by which mouth breathing affects palate descent from an aerodynamic perspective. Cone beam computed tomography (CBCT) data were used to reconstruct a 3-dimensional model during natural mouth breathing of a volunteer. The model was imported into CFX 19.

Does Preoperative Activity Level Affect Postoperative Clinical Outcomes Following Hip Arthroscopy in Femoroacetabular Impingement Syndrome (FAIS) Patients?

Objective: When considering surgical treatment options, many patients who undergo hip arthroscopy value continuing active lifestyles. To address these concerns, the purpose of this study was to determine the effect of preoperative activity level on postoperative patient-reported outcomes (PROs) in femoroacetabular impingement syndrome (FAIS) patients following hip arthroscopy.

Methods: Data was retrospectively reviewed for FAIS patients who received hip arthroscopy between 2016 and 2018.

Outcomes in Patients with Global Pincer Versus Focal Pincer Femoroacetabular Impingement Treated with Hip Arthroscopy: A Retrospective Study with a Minimum 2-Year Follow-Up.

Objective: Global pincer is a relatively rare form of pincer deformity and is typically associated with technical challenges during surgery. So far, controversy remains whether patients with global pincer have equivalent surgical outcomes compared to patients with focal pincer. This study compares the clinical outcomes of arthroscopic treatment between patients with global pincer femoroacetabular impingement (FAI) and focal pincer FAI in the Chinese population.

Parallel optical coherent dot-product architecture for large-scale matrix multiplication with compatibility for diverse phase shifters.

Photonics physically promises high-speed and low-consumption computing of matrix multiplication. Nevertheless, conventional approaches are challenging to achieve large throughput, high precision, low power consumption, and high density simultaneously in a single architecture, because the integration scale of conventional approaches is strongly limited by the insertion loss of cascaded optical phase shifters. Here, we present a parallel optical coherent dot-product (P-OCD) architecture, which deploys phase shifters in a fully parallel way.

Inhibition of LDL receptor-related protein 3 suppresses chondrogenesis of stem cells, inhibits proliferation, and promotes apoptosis.

Articular cartilage defects remain the most common and challenging joint disease. Cartilage lacks the self-healing capacity after injury due to its avascularity. Recently, stem cell-based therapy has been applied for cartilage regeneration.

Better Coverage of the ACL Tibial Footprint and Less Injury to the Anterior Root of the Lateral Meniscus Using a Rounded-Rectangular Tibial Tunnel in ACL Reconstruction: A Cadaveric Study.

Background: To better restore the anatomy of the native anterior cruciate ligament (ACL) attachment and fiber arrangement, researchers have developed techniques for changing the shape of the ACL bone tunnel during ACL reconstruction.

Purpose: To compare the coverage of the ACL tibial footprint and influence on the anterior root of lateral meniscus (ARLM) between a rounded-rectangular tibial tunnel and a conventional round tibial tunnel for ACL reconstruction.

Study Design: Controlled laboratory study.

Flat-Tunnel Technique With Independently Tensioned Bundles Better Restores Rotational Stability Than Round-Tunnel Technique in Anatomic Anterior Cruciate Ligament Reconstruction Using Hamstring Graft: A Cadaveric Biomechanical Study.

Purpose: To investigate the kinematics differences between round-tunnel (ROT) and flat-tunnel (FLT) techniques in anterior cruciate ligament (ACL) reconstruction when using hamstring graft.

Methods: Nine matched pairs of fresh-frozen cadaveric knees were evaluated for the kinematics of intact, ACL-sectioned, and either ROT or FLT reconstructed knees. The graft bundles for FLT technique were separately tensioned.

[Non-structural carbohydrate content of trees and its influencing factors at multiple spatial-temporal scales: A review].

Non-structural carbohydrates (NSC) are essential substances for the tree growth and metabolism, and play an important role in environmental adaptation of trees. At temporal scale, NSC contents in trees have limited inter-annual variation, which could be attributed to the strategy of tree growth and carbon storage. Different factors influence NSC contents of trees in various climatic regions, which change substantially at the seasonal scale.

Asymmetric 3D Elastic-Plastic Strain-Modulated Electron Energy Structure in Monolayer Graphene by Laser Shocking.

Graphene has a great potential to replace silicon in prospective semiconductor industries due to its outstanding electronic and transport properties; nonetheless, its lack of energy bandgap is a substantial limitation for practical applications. To date, straining graphene to break its lattice symmetry is perhaps the most efficient approach toward realizing bandgap tunability in graphene. However, due to the weak lattice deformation induced by uniaxial or in-plane shear strain, most strained graphene studies have yielded bandgaps <1 eV.

Direct laser writing of nanodiamond films from graphite under ambient conditions.

Synthesis of diamond, a multi-functional material, has been a challenge due to very high activation energy for transforming graphite to diamond, and therefore, has been hindering it from being potentially exploited for novel applications. In this study, we explore a new approach, namely confined pulse laser deposition (CPLD), in which nanosecond laser ablation of graphite within a confinement layer simultaneously activates plasma and effectively confine it to create a favorable condition for nanodiamond formation from graphite. It is noteworthy that due to the local high dense confined plasma created by transparent confinement layer, nanodiamond has been formed at laser intensity as low as 3.

Ultrafast and scalable laser liquid synthesis of tin oxide nanotubes and its application in lithium ion batteries.

Laser-induced photo-chemical synthesis of SnO2 nanotubes has been demonstrated by employing a nanoporous polycarbonate membrane as a template. The SnO2 nanotube diameter can be controlled by the nanoporous template while the nanotube length can be tuned by laser parameters and reaction duration. The microstructure characterization of the nanotubes indicates that they consist of mesoporous structures with sub 5 nm size nanocrystals connected by the twinning structure.